Describing the intestinal microbiota of Holstein Fasciola-positive and -negative cattle from a hyperendemic area of fascioliasis in central Colombia.

The ability to identify compositional changes in the intestinal microbiota of parasitized hosts is important for understanding the physiological processes that may affect animal productivity. Within the field of host-parasite interactions, many studies have suggested that helminths can influence the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Angie L Ramírez, Giovanny Herrera, Marina Muñoz, Laura Vega, Lissa Cruz-Saavedra, Diego García-Corredor, Martin Pulido-Medellín, Diana M Bulla-Castañeda, Julio Cesar Giraldo, María Consuelo Bernal, José Fernández-Manrique, Luis R Vásquez-Arteaga, Juan David Ramírez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/5f81521444444af7a031be7485cd9754
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5f81521444444af7a031be7485cd9754
record_format	dspace
spelling	oai:doaj.org-article:5f81521444444af7a031be7485cd97542021-12-02T20:23:41ZDescribing the intestinal microbiota of Holstein Fasciola-positive and -negative cattle from a hyperendemic area of fascioliasis in central Colombia.1935-27271935-273510.1371/journal.pntd.0009658https://doaj.org/article/5f81521444444af7a031be7485cd97542021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009658https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735The ability to identify compositional changes in the intestinal microbiota of parasitized hosts is important for understanding the physiological processes that may affect animal productivity. Within the field of host-parasite interactions, many studies have suggested that helminths can influence the microbial composition of their hosts via their immunomodulatory effects. Bovine fascioliasis is a helminthiasis widely studied by immunologists, but with little information available regarding gut microbial communities. Thus, we aimed to describe the composition of the intestinal microbiota of Holstein Fasciola-positive and -negative cattle using parasitological methods and ELISA (enzyme-linked immunosorbent assay). Bovine fecal samples (n = 65) were obtained from livestock slaughter plants in the Cundi-Boyacense Colombian highlands (a hyperendemic region for bovine fascioliasis) and studied by amplicon-based next-generation 16S-rRNA and 18S-rRNA gene sequencing. From these samples, 35 were Fasciola hepatica-negative and, 30 were F. hepatica-positive in our detection analysis. Our results showed a reduction in the relative abundance of Bacteroidetes and Ascomycota in the Fasciola-positive samples, along with decreased relative abundances of the commensal taxa previously associated with fermentation and digestion processes. However, metabolomic approaches and functional analyzes of the intestinal microbiota are necessary to support these hypothesis. These findings are a small first step in the development of research aimed at understanding how microbial populations in bovines are modulated in liver helminth infections.Angie L RamírezGiovanny HerreraMarina MuñozLaura VegaLissa Cruz-SaavedraDiego García-CorredorMartin Pulido-MedellínDiana M Bulla-CastañedaJulio Cesar GiraldoMaría Consuelo BernalJosé Fernández-ManriqueLuis R Vásquez-ArteagaJuan David RamírezPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 8, p e0009658 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Angie L Ramírez Giovanny Herrera Marina Muñoz Laura Vega Lissa Cruz-Saavedra Diego García-Corredor Martin Pulido-Medellín Diana M Bulla-Castañeda Julio Cesar Giraldo María Consuelo Bernal José Fernández-Manrique Luis R Vásquez-Arteaga Juan David Ramírez Describing the intestinal microbiota of Holstein Fasciola-positive and -negative cattle from a hyperendemic area of fascioliasis in central Colombia.
description	The ability to identify compositional changes in the intestinal microbiota of parasitized hosts is important for understanding the physiological processes that may affect animal productivity. Within the field of host-parasite interactions, many studies have suggested that helminths can influence the microbial composition of their hosts via their immunomodulatory effects. Bovine fascioliasis is a helminthiasis widely studied by immunologists, but with little information available regarding gut microbial communities. Thus, we aimed to describe the composition of the intestinal microbiota of Holstein Fasciola-positive and -negative cattle using parasitological methods and ELISA (enzyme-linked immunosorbent assay). Bovine fecal samples (n = 65) were obtained from livestock slaughter plants in the Cundi-Boyacense Colombian highlands (a hyperendemic region for bovine fascioliasis) and studied by amplicon-based next-generation 16S-rRNA and 18S-rRNA gene sequencing. From these samples, 35 were Fasciola hepatica-negative and, 30 were F. hepatica-positive in our detection analysis. Our results showed a reduction in the relative abundance of Bacteroidetes and Ascomycota in the Fasciola-positive samples, along with decreased relative abundances of the commensal taxa previously associated with fermentation and digestion processes. However, metabolomic approaches and functional analyzes of the intestinal microbiota are necessary to support these hypothesis. These findings are a small first step in the development of research aimed at understanding how microbial populations in bovines are modulated in liver helminth infections.
format	article
author	Angie L Ramírez Giovanny Herrera Marina Muñoz Laura Vega Lissa Cruz-Saavedra Diego García-Corredor Martin Pulido-Medellín Diana M Bulla-Castañeda Julio Cesar Giraldo María Consuelo Bernal José Fernández-Manrique Luis R Vásquez-Arteaga Juan David Ramírez
author_facet	Angie L Ramírez Giovanny Herrera Marina Muñoz Laura Vega Lissa Cruz-Saavedra Diego García-Corredor Martin Pulido-Medellín Diana M Bulla-Castañeda Julio Cesar Giraldo María Consuelo Bernal José Fernández-Manrique Luis R Vásquez-Arteaga Juan David Ramírez
author_sort	Angie L Ramírez
title	Describing the intestinal microbiota of Holstein Fasciola-positive and -negative cattle from a hyperendemic area of fascioliasis in central Colombia.
title_short	Describing the intestinal microbiota of Holstein Fasciola-positive and -negative cattle from a hyperendemic area of fascioliasis in central Colombia.
title_full	Describing the intestinal microbiota of Holstein Fasciola-positive and -negative cattle from a hyperendemic area of fascioliasis in central Colombia.
title_fullStr	Describing the intestinal microbiota of Holstein Fasciola-positive and -negative cattle from a hyperendemic area of fascioliasis in central Colombia.
title_full_unstemmed	Describing the intestinal microbiota of Holstein Fasciola-positive and -negative cattle from a hyperendemic area of fascioliasis in central Colombia.
title_sort	describing the intestinal microbiota of holstein fasciola-positive and -negative cattle from a hyperendemic area of fascioliasis in central colombia.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5f81521444444af7a031be7485cd9754
work_keys_str_mv	AT angielramirez describingtheintestinalmicrobiotaofholsteinfasciolapositiveandnegativecattlefromahyperendemicareaoffascioliasisincentralcolombia AT giovannyherrera describingtheintestinalmicrobiotaofholsteinfasciolapositiveandnegativecattlefromahyperendemicareaoffascioliasisincentralcolombia AT marinamunoz describingtheintestinalmicrobiotaofholsteinfasciolapositiveandnegativecattlefromahyperendemicareaoffascioliasisincentralcolombia AT lauravega describingtheintestinalmicrobiotaofholsteinfasciolapositiveandnegativecattlefromahyperendemicareaoffascioliasisincentralcolombia AT lissacruzsaavedra describingtheintestinalmicrobiotaofholsteinfasciolapositiveandnegativecattlefromahyperendemicareaoffascioliasisincentralcolombia AT diegogarciacorredor describingtheintestinalmicrobiotaofholsteinfasciolapositiveandnegativecattlefromahyperendemicareaoffascioliasisincentralcolombia AT martinpulidomedellin describingtheintestinalmicrobiotaofholsteinfasciolapositiveandnegativecattlefromahyperendemicareaoffascioliasisincentralcolombia AT dianambullacastaneda describingtheintestinalmicrobiotaofholsteinfasciolapositiveandnegativecattlefromahyperendemicareaoffascioliasisincentralcolombia AT juliocesargiraldo describingtheintestinalmicrobiotaofholsteinfasciolapositiveandnegativecattlefromahyperendemicareaoffascioliasisincentralcolombia AT mariaconsuelobernal describingtheintestinalmicrobiotaofholsteinfasciolapositiveandnegativecattlefromahyperendemicareaoffascioliasisincentralcolombia AT josefernandezmanrique describingtheintestinalmicrobiotaofholsteinfasciolapositiveandnegativecattlefromahyperendemicareaoffascioliasisincentralcolombia AT luisrvasquezarteaga describingtheintestinalmicrobiotaofholsteinfasciolapositiveandnegativecattlefromahyperendemicareaoffascioliasisincentralcolombia AT juandavidramirez describingtheintestinalmicrobiotaofholsteinfasciolapositiveandnegativecattlefromahyperendemicareaoffascioliasisincentralcolombia
_version_	1718374110199808000

Describing the intestinal microbiota of Holstein Fasciola-positive and -negative cattle from a hyperendemic area of fascioliasis in central Colombia.

Ejemplares similares