UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection

Summary: Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Roberto Alcántara, Katherin Peñaranda, Gabriel Mendoza-Rojas, Jose A. Nakamoto, Eva Dueñas, Daniela Alvarez, Vanessa Adaui, Pohl Milón
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Clinical Protocol Immunology Microbiology Molecular Biology CRISPR Biotechnology and bioengineering Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/5f855e9ac88b4fc5bc14c0001c76f1d7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5f855e9ac88b4fc5bc14c0001c76f1d7
record_format	dspace
spelling	oai:doaj.org-article:5f855e9ac88b4fc5bc14c0001c76f1d72021-11-04T04:39:44ZUnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection2666-166710.1016/j.xpro.2021.100878https://doaj.org/article/5f855e9ac88b4fc5bc14c0001c76f1d72021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721005840https://doaj.org/toc/2666-1667Summary: Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab.For complete details on the use and execution of this protocol, please refer to Alcántara et al., 2021.Roberto AlcántaraKatherin PeñarandaGabriel Mendoza-RojasJose A. NakamotoEva DueñasDaniela AlvarezVanessa AdauiPohl MilónElsevierarticleClinical ProtocolImmunologyMicrobiologyMolecular BiologyCRISPRBiotechnology and bioengineeringScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100878- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Clinical Protocol Immunology Microbiology Molecular Biology CRISPR Biotechnology and bioengineering Science (General) Q1-390
spellingShingle	Clinical Protocol Immunology Microbiology Molecular Biology CRISPR Biotechnology and bioengineering Science (General) Q1-390 Roberto Alcántara Katherin Peñaranda Gabriel Mendoza-Rojas Jose A. Nakamoto Eva Dueñas Daniela Alvarez Vanessa Adaui Pohl Milón UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
description	Summary: Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab.For complete details on the use and execution of this protocol, please refer to Alcántara et al., 2021.
format	article
author	Roberto Alcántara Katherin Peñaranda Gabriel Mendoza-Rojas Jose A. Nakamoto Eva Dueñas Daniela Alvarez Vanessa Adaui Pohl Milón
author_facet	Roberto Alcántara Katherin Peñaranda Gabriel Mendoza-Rojas Jose A. Nakamoto Eva Dueñas Daniela Alvarez Vanessa Adaui Pohl Milón
author_sort	Roberto Alcántara
title	UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
title_short	UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
title_full	UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
title_fullStr	UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
title_full_unstemmed	UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
title_sort	uncovid: a versatile, low-cost, and open-source protocol for sars-cov-2 rna detection
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/5f855e9ac88b4fc5bc14c0001c76f1d7
work_keys_str_mv	AT robertoalcantara uncovidaversatilelowcostandopensourceprotocolforsarscov2rnadetection AT katherinpenaranda uncovidaversatilelowcostandopensourceprotocolforsarscov2rnadetection AT gabrielmendozarojas uncovidaversatilelowcostandopensourceprotocolforsarscov2rnadetection AT joseanakamoto uncovidaversatilelowcostandopensourceprotocolforsarscov2rnadetection AT evaduenas uncovidaversatilelowcostandopensourceprotocolforsarscov2rnadetection AT danielaalvarez uncovidaversatilelowcostandopensourceprotocolforsarscov2rnadetection AT vanessaadaui uncovidaversatilelowcostandopensourceprotocolforsarscov2rnadetection AT pohlmilon uncovidaversatilelowcostandopensourceprotocolforsarscov2rnadetection
_version_	1718445214758076416

UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection

Ejemplares similares