UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
Summary: Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection....
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Autores principales: | Roberto Alcántara, Katherin Peñaranda, Gabriel Mendoza-Rojas, Jose A. Nakamoto, Eva Dueñas, Daniela Alvarez, Vanessa Adaui, Pohl Milón |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://doaj.org/article/5f855e9ac88b4fc5bc14c0001c76f1d7 |
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