UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
Summary: Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection....
Enregistré dans:
Auteurs principaux: | Roberto Alcántara, Katherin Peñaranda, Gabriel Mendoza-Rojas, Jose A. Nakamoto, Eva Dueñas, Daniela Alvarez, Vanessa Adaui, Pohl Milón |
---|---|
Format: | article |
Langue: | EN |
Publié: |
Elsevier
2021
|
Sujets: | |
Accès en ligne: | https://doaj.org/article/5f855e9ac88b4fc5bc14c0001c76f1d7 |
Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|
Documents similaires
-
A low-cost and open-source protocol to produce key enzymes for molecular detection assays
par: Gabriel Mendoza-Rojas, et autres
Publié: (2021) -
Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
par: Daiyu Ito, et autres
Publié: (2021) -
Generation of auxin inducible degron (AID) knock-in cell lines for targeted protein degradation in mammalian cells
par: Bikash Adhikari, et autres
Publié: (2021) - AIMS bioengineering
-
Optimized electroporation of CRISPR-Cas9/gRNA ribonucleoprotein complex for selection-free homologous recombination in human pluripotent stem cells
par: Huaigeng Xu, et autres
Publié: (2021)