Proteome and strain analysis of cyanobacterium Candidatus “Phormidium alkaliphilum” reveals traits for success in biotechnology

Summary: Cyanobacteria encompass a diverse group of photoautotrophic bacteria with important roles in nature and biotechnology. Here we characterized Candidatus “Phormidium alkaliphilum,” an abundant member in alkaline soda lake microbial communities globally. The complete, circular whole-genome seq...

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Autores principales:	Maryam Ataeian, Agasteswar Vadlamani, Marianne Haines, Damon Mosier, Xiaoli Dong, Manuel Kleiner, Marc Strous, Alyse K. Hawley
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Genomics Microbial biotechnology Proteomics Cyanobacteria Science Q
Acceso en línea:	https://doaj.org/article/5f8e8e2a7e44478398df8cf9f28988b0
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spelling	oai:doaj.org-article:5f8e8e2a7e44478398df8cf9f28988b02021-11-28T04:36:16ZProteome and strain analysis of cyanobacterium Candidatus “Phormidium alkaliphilum” reveals traits for success in biotechnology2589-004210.1016/j.isci.2021.103405https://doaj.org/article/5f8e8e2a7e44478398df8cf9f28988b02021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221013766https://doaj.org/toc/2589-0042Summary: Cyanobacteria encompass a diverse group of photoautotrophic bacteria with important roles in nature and biotechnology. Here we characterized Candidatus “Phormidium alkaliphilum,” an abundant member in alkaline soda lake microbial communities globally. The complete, circular whole-genome sequence of Ca. “P. alkaliphilum” was obtained using combined Nanopore and Illumina sequencing of a Ca. “P. alkaliphilum” consortium. Strain-level diversity of Ca. “P. alkaliphilum” was shown to contribute to photobioreactor robustness under different operational conditions. Comparative genomics of closely related species showed that adaptation to high pH was not attributed to specific genes. Proteomics at high and low pH showed only minimal changes in gene expression, but higher productivity in high pH. Diverse photosystem antennae proteins, and high-affinity terminal oxidase, compared with other soda lake cyanobacteria, appear to contribute to the success of Ca. “P. alkaliphilum” in photobioreactors and biotechnology applications.Maryam AtaeianAgasteswar VadlamaniMarianne HainesDamon MosierXiaoli DongManuel KleinerMarc StrousAlyse K. HawleyElsevierarticleGenomicsMicrobial biotechnologyProteomicsCyanobacteriaScienceQENiScience, Vol 24, Iss 12, Pp 103405- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genomics Microbial biotechnology Proteomics Cyanobacteria Science Q
spellingShingle	Genomics Microbial biotechnology Proteomics Cyanobacteria Science Q Maryam Ataeian Agasteswar Vadlamani Marianne Haines Damon Mosier Xiaoli Dong Manuel Kleiner Marc Strous Alyse K. Hawley Proteome and strain analysis of cyanobacterium Candidatus “Phormidium alkaliphilum” reveals traits for success in biotechnology
description	Summary: Cyanobacteria encompass a diverse group of photoautotrophic bacteria with important roles in nature and biotechnology. Here we characterized Candidatus “Phormidium alkaliphilum,” an abundant member in alkaline soda lake microbial communities globally. The complete, circular whole-genome sequence of Ca. “P. alkaliphilum” was obtained using combined Nanopore and Illumina sequencing of a Ca. “P. alkaliphilum” consortium. Strain-level diversity of Ca. “P. alkaliphilum” was shown to contribute to photobioreactor robustness under different operational conditions. Comparative genomics of closely related species showed that adaptation to high pH was not attributed to specific genes. Proteomics at high and low pH showed only minimal changes in gene expression, but higher productivity in high pH. Diverse photosystem antennae proteins, and high-affinity terminal oxidase, compared with other soda lake cyanobacteria, appear to contribute to the success of Ca. “P. alkaliphilum” in photobioreactors and biotechnology applications.
format	article
author	Maryam Ataeian Agasteswar Vadlamani Marianne Haines Damon Mosier Xiaoli Dong Manuel Kleiner Marc Strous Alyse K. Hawley
author_facet	Maryam Ataeian Agasteswar Vadlamani Marianne Haines Damon Mosier Xiaoli Dong Manuel Kleiner Marc Strous Alyse K. Hawley
author_sort	Maryam Ataeian
title	Proteome and strain analysis of cyanobacterium Candidatus “Phormidium alkaliphilum” reveals traits for success in biotechnology
title_short	Proteome and strain analysis of cyanobacterium Candidatus “Phormidium alkaliphilum” reveals traits for success in biotechnology
title_full	Proteome and strain analysis of cyanobacterium Candidatus “Phormidium alkaliphilum” reveals traits for success in biotechnology
title_fullStr	Proteome and strain analysis of cyanobacterium Candidatus “Phormidium alkaliphilum” reveals traits for success in biotechnology
title_full_unstemmed	Proteome and strain analysis of cyanobacterium Candidatus “Phormidium alkaliphilum” reveals traits for success in biotechnology
title_sort	proteome and strain analysis of cyanobacterium candidatus “phormidium alkaliphilum” reveals traits for success in biotechnology
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/5f8e8e2a7e44478398df8cf9f28988b0
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Proteome and strain analysis of cyanobacterium Candidatus “Phormidium alkaliphilum” reveals traits for success in biotechnology

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