Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for tumor stages detection and histologic grades classification of oral squamous cell carcinoma

Lili Xue,1 Bing Yan,2 Yi Li,3 Yingyun Tan,4 Xianyang Luo,2 Min Wang1 1State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China; 2Department of Ot...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xue L, Yan B, Li Y, Tan Y, Luo X, Wang M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	SERS OSCC TNM classification Histologic grades Diagnosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5f904d4e2c674855bd87861f2592e4cd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5f904d4e2c674855bd87861f2592e4cd
record_format	dspace
spelling	oai:doaj.org-article:5f904d4e2c674855bd87861f2592e4cd2021-12-02T06:21:08ZSurface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for tumor stages detection and histologic grades classification of oral squamous cell carcinoma1178-2013https://doaj.org/article/5f904d4e2c674855bd87861f2592e4cd2018-08-01T00:00:00Zhttps://www.dovepress.com/surface-enhanced-raman-spectroscopy-of-blood-serum-based-on-gold-nanop-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Lili Xue,1 Bing Yan,2 Yi Li,3 Yingyun Tan,4 Xianyang Luo,2 Min Wang1 1State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China; 2Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, China; 3Department of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, Chengdu, China; 4Department of Implant, Stomatological Hospital of Chongqing Medical University, Chongqing, China Background: Tumor stages detection and histologic grades classification are essential for the diagnosis and prognosis of oral squamous cell carcinoma (OSCC). In this research, we apply surface-enhanced Raman spectroscopy (SERS) of blood serum to detect the tumor stages and histologic classification of OSCC. Methods: According to TNM classification and World Health Organization histologic grading system, the blood serum samples were collected from a total of 135 OSCC patients in the different tumor stages and histologic grades. Then the SERS spectra of serum samples from OSCC patients were diagnosed and classified into different groups using principal component analysis (PCA) and linear discriminant analysis (LDA) based on the tumor sizes, lymph node metastasis and histologic grades. Results: The SERS spectra of blood serum samples have shown the distinct changes and differences compared with each other, which were assigned to the biomolecule alterations (nucleic acids, proteins, lipids, and so on) in blood serums. And all accuracies of detection and classification reached above 85%. Conclusion: This study demonstrated that the SERS based on blood serum test had an enormous potential to carry out the preoperative assessment and prediction of the OSCC patients in different tumor stages and histologic classification. Keywords: SERS, OSCC, TNM classification, histologic grades, diagnosisXue LYan BLi YTan YLuo XWang MDove Medical PressarticleSERSOSCCTNM classificationHistologic gradesDiagnosisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 4977-4986 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	SERS OSCC TNM classification Histologic grades Diagnosis Medicine (General) R5-920
spellingShingle	SERS OSCC TNM classification Histologic grades Diagnosis Medicine (General) R5-920 Xue L Yan B Li Y Tan Y Luo X Wang M Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for tumor stages detection and histologic grades classification of oral squamous cell carcinoma
description	Lili Xue,1 Bing Yan,2 Yi Li,3 Yingyun Tan,4 Xianyang Luo,2 Min Wang1 1State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China; 2Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, China; 3Department of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, Chengdu, China; 4Department of Implant, Stomatological Hospital of Chongqing Medical University, Chongqing, China Background: Tumor stages detection and histologic grades classification are essential for the diagnosis and prognosis of oral squamous cell carcinoma (OSCC). In this research, we apply surface-enhanced Raman spectroscopy (SERS) of blood serum to detect the tumor stages and histologic classification of OSCC. Methods: According to TNM classification and World Health Organization histologic grading system, the blood serum samples were collected from a total of 135 OSCC patients in the different tumor stages and histologic grades. Then the SERS spectra of serum samples from OSCC patients were diagnosed and classified into different groups using principal component analysis (PCA) and linear discriminant analysis (LDA) based on the tumor sizes, lymph node metastasis and histologic grades. Results: The SERS spectra of blood serum samples have shown the distinct changes and differences compared with each other, which were assigned to the biomolecule alterations (nucleic acids, proteins, lipids, and so on) in blood serums. And all accuracies of detection and classification reached above 85%. Conclusion: This study demonstrated that the SERS based on blood serum test had an enormous potential to carry out the preoperative assessment and prediction of the OSCC patients in different tumor stages and histologic classification. Keywords: SERS, OSCC, TNM classification, histologic grades, diagnosis
format	article
author	Xue L Yan B Li Y Tan Y Luo X Wang M
author_facet	Xue L Yan B Li Y Tan Y Luo X Wang M
author_sort	Xue L
title	Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for tumor stages detection and histologic grades classification of oral squamous cell carcinoma
title_short	Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for tumor stages detection and histologic grades classification of oral squamous cell carcinoma
title_full	Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for tumor stages detection and histologic grades classification of oral squamous cell carcinoma
title_fullStr	Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for tumor stages detection and histologic grades classification of oral squamous cell carcinoma
title_full_unstemmed	Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for tumor stages detection and histologic grades classification of oral squamous cell carcinoma
title_sort	surface-enhanced raman spectroscopy of blood serum based on gold nanoparticles for tumor stages detection and histologic grades classification of oral squamous cell carcinoma
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/5f904d4e2c674855bd87861f2592e4cd
work_keys_str_mv	AT xuel surfaceenhancedramanspectroscopyofbloodserumbasedongoldnanoparticlesfortumorstagesdetectionandhistologicgradesclassificationoforalsquamouscellcarcinoma AT yanb surfaceenhancedramanspectroscopyofbloodserumbasedongoldnanoparticlesfortumorstagesdetectionandhistologicgradesclassificationoforalsquamouscellcarcinoma AT liy surfaceenhancedramanspectroscopyofbloodserumbasedongoldnanoparticlesfortumorstagesdetectionandhistologicgradesclassificationoforalsquamouscellcarcinoma AT tany surfaceenhancedramanspectroscopyofbloodserumbasedongoldnanoparticlesfortumorstagesdetectionandhistologicgradesclassificationoforalsquamouscellcarcinoma AT luox surfaceenhancedramanspectroscopyofbloodserumbasedongoldnanoparticlesfortumorstagesdetectionandhistologicgradesclassificationoforalsquamouscellcarcinoma AT wangm surfaceenhancedramanspectroscopyofbloodserumbasedongoldnanoparticlesfortumorstagesdetectionandhistologicgradesclassificationoforalsquamouscellcarcinoma
_version_	1718399934954209280

Surface-enhanced Raman spectroscopy of blood serum based on gold nanoparticles for tumor stages detection and histologic grades classification of oral squamous cell carcinoma

Ejemplares similares