A role in immunity for Arabidopsis cysteine protease RD21, the ortholog of the tomato immune protease C14.

Secreted papain-like Cys proteases are important players in plant immunity. We previously reported that the C14 protease of tomato is targeted by cystatin-like EPIC proteins that are secreted by the oomycete pathogen Phytophthora infestans (Pinf) during infection. C14 has been under diversifying sel...

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Autores principales:	Takayuki Shindo, Johana C Misas-Villamil, Anja C Hörger, Jing Song, Renier A L van der Hoorn
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f969afec265404f8f363671501ec15a
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spelling	oai:doaj.org-article:5f969afec265404f8f363671501ec15a2021-11-18T07:30:48ZA role in immunity for Arabidopsis cysteine protease RD21, the ortholog of the tomato immune protease C14.1932-620310.1371/journal.pone.0029317https://doaj.org/article/5f969afec265404f8f363671501ec15a2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22238602/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Secreted papain-like Cys proteases are important players in plant immunity. We previously reported that the C14 protease of tomato is targeted by cystatin-like EPIC proteins that are secreted by the oomycete pathogen Phytophthora infestans (Pinf) during infection. C14 has been under diversifying selection in wild potato species coevolving with Pinf and reduced C14 levels result in enhanced susceptibility for Pinf. Here, we investigated the role C14-EPIC-like interactions in the natural pathosystem of Arabidopsis with the oomycete pathogen Hyaloperonospora arabidopsidis (Hpa). In contrast to the Pinf-solanaceae pathosystem, the C14 orthologous protease of Arabidopsis, RD21, does not evolve under diversifying selection in Arabidopsis, and rd21 null mutants do not show phenotypes upon compatible and incompatible Hpa interactions, despite the evident lack of a major leaf protease. Hpa isolates express highly conserved EPIC-like proteins during infections, but it is unknown if these HpaEPICs can inhibit RD21 and one of these HpaEPICs even lacks the canonical cystatin motifs. The rd21 mutants are unaffected in compatible and incompatible interactions with Pseudomonas syringae pv. tomato, but are significantly more susceptible for the necrotrophic fungal pathogen Botrytis cinerea, demonstrating that RD21 provides immunity to a necrotrophic pathogen.Takayuki ShindoJohana C Misas-VillamilAnja C HörgerJing SongRenier A L van der HoornPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e29317 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takayuki Shindo Johana C Misas-Villamil Anja C Hörger Jing Song Renier A L van der Hoorn A role in immunity for Arabidopsis cysteine protease RD21, the ortholog of the tomato immune protease C14.
description	Secreted papain-like Cys proteases are important players in plant immunity. We previously reported that the C14 protease of tomato is targeted by cystatin-like EPIC proteins that are secreted by the oomycete pathogen Phytophthora infestans (Pinf) during infection. C14 has been under diversifying selection in wild potato species coevolving with Pinf and reduced C14 levels result in enhanced susceptibility for Pinf. Here, we investigated the role C14-EPIC-like interactions in the natural pathosystem of Arabidopsis with the oomycete pathogen Hyaloperonospora arabidopsidis (Hpa). In contrast to the Pinf-solanaceae pathosystem, the C14 orthologous protease of Arabidopsis, RD21, does not evolve under diversifying selection in Arabidopsis, and rd21 null mutants do not show phenotypes upon compatible and incompatible Hpa interactions, despite the evident lack of a major leaf protease. Hpa isolates express highly conserved EPIC-like proteins during infections, but it is unknown if these HpaEPICs can inhibit RD21 and one of these HpaEPICs even lacks the canonical cystatin motifs. The rd21 mutants are unaffected in compatible and incompatible interactions with Pseudomonas syringae pv. tomato, but are significantly more susceptible for the necrotrophic fungal pathogen Botrytis cinerea, demonstrating that RD21 provides immunity to a necrotrophic pathogen.
format	article
author	Takayuki Shindo Johana C Misas-Villamil Anja C Hörger Jing Song Renier A L van der Hoorn
author_facet	Takayuki Shindo Johana C Misas-Villamil Anja C Hörger Jing Song Renier A L van der Hoorn
author_sort	Takayuki Shindo
title	A role in immunity for Arabidopsis cysteine protease RD21, the ortholog of the tomato immune protease C14.
title_short	A role in immunity for Arabidopsis cysteine protease RD21, the ortholog of the tomato immune protease C14.
title_full	A role in immunity for Arabidopsis cysteine protease RD21, the ortholog of the tomato immune protease C14.
title_fullStr	A role in immunity for Arabidopsis cysteine protease RD21, the ortholog of the tomato immune protease C14.
title_full_unstemmed	A role in immunity for Arabidopsis cysteine protease RD21, the ortholog of the tomato immune protease C14.
title_sort	role in immunity for arabidopsis cysteine protease rd21, the ortholog of the tomato immune protease c14.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/5f969afec265404f8f363671501ec15a
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A role in immunity for Arabidopsis cysteine protease RD21, the ortholog of the tomato immune protease C14.

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