Epigenetic regulation of starvation-induced autophagy in Drosophila by histone methyltransferase G9a

Abstract Epigenetics is now emerging as a key regulation in response to various stresses. We herein identified the Drosophila histone methyltransferase G9a (dG9a) as a key factor to acquire tolerance to starvation stress. The depletion of dG9a led to high sensitivity to starvation stress in adult fl...

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Autores principales:	Phan Nguyen Thuy An, Kouhei Shimaji, Ryo Tanaka, Hideki Yoshida, Hiroshi Kimura, Eiichiro Fukusaki, Masamitsu Yamaguchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f97ee86ce27437db572b832c7291c21
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spelling	oai:doaj.org-article:5f97ee86ce27437db572b832c7291c212021-12-02T11:40:30ZEpigenetic regulation of starvation-induced autophagy in Drosophila by histone methyltransferase G9a10.1038/s41598-017-07566-12045-2322https://doaj.org/article/5f97ee86ce27437db572b832c7291c212017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07566-1https://doaj.org/toc/2045-2322Abstract Epigenetics is now emerging as a key regulation in response to various stresses. We herein identified the Drosophila histone methyltransferase G9a (dG9a) as a key factor to acquire tolerance to starvation stress. The depletion of dG9a led to high sensitivity to starvation stress in adult flies, while its overexpression induced starvation stress resistance. The catalytic domain of dG9a was not required for starvation stress resistance. dG9a plays no apparent role in tolerance to other stresses including heat and oxidative stresses. Metabolomic approaches were applied to investigate global changes in the metabolome due to the loss of dG9a during starvation stress. The results obtained indicated that dG9a plays an important role in maintaining energy reservoirs including amino acid, trehalose, glycogen, and triacylglycerol levels during starvation. Further investigations on the underlying mechanisms showed that the depletion of dG9a repressed starvation-induced autophagy by controlling the expression level of Atg8a, a critical gene for the progression of autophagy, in a different manner to that in cancer cells. These results indicate a positive role for dG9a in starvation-induced autophagy.Phan Nguyen Thuy AnKouhei ShimajiRyo TanakaHideki YoshidaHiroshi KimuraEiichiro FukusakiMasamitsu YamaguchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Phan Nguyen Thuy An Kouhei Shimaji Ryo Tanaka Hideki Yoshida Hiroshi Kimura Eiichiro Fukusaki Masamitsu Yamaguchi Epigenetic regulation of starvation-induced autophagy in Drosophila by histone methyltransferase G9a
description	Abstract Epigenetics is now emerging as a key regulation in response to various stresses. We herein identified the Drosophila histone methyltransferase G9a (dG9a) as a key factor to acquire tolerance to starvation stress. The depletion of dG9a led to high sensitivity to starvation stress in adult flies, while its overexpression induced starvation stress resistance. The catalytic domain of dG9a was not required for starvation stress resistance. dG9a plays no apparent role in tolerance to other stresses including heat and oxidative stresses. Metabolomic approaches were applied to investigate global changes in the metabolome due to the loss of dG9a during starvation stress. The results obtained indicated that dG9a plays an important role in maintaining energy reservoirs including amino acid, trehalose, glycogen, and triacylglycerol levels during starvation. Further investigations on the underlying mechanisms showed that the depletion of dG9a repressed starvation-induced autophagy by controlling the expression level of Atg8a, a critical gene for the progression of autophagy, in a different manner to that in cancer cells. These results indicate a positive role for dG9a in starvation-induced autophagy.
format	article
author	Phan Nguyen Thuy An Kouhei Shimaji Ryo Tanaka Hideki Yoshida Hiroshi Kimura Eiichiro Fukusaki Masamitsu Yamaguchi
author_facet	Phan Nguyen Thuy An Kouhei Shimaji Ryo Tanaka Hideki Yoshida Hiroshi Kimura Eiichiro Fukusaki Masamitsu Yamaguchi
author_sort	Phan Nguyen Thuy An
title	Epigenetic regulation of starvation-induced autophagy in Drosophila by histone methyltransferase G9a
title_short	Epigenetic regulation of starvation-induced autophagy in Drosophila by histone methyltransferase G9a
title_full	Epigenetic regulation of starvation-induced autophagy in Drosophila by histone methyltransferase G9a
title_fullStr	Epigenetic regulation of starvation-induced autophagy in Drosophila by histone methyltransferase G9a
title_full_unstemmed	Epigenetic regulation of starvation-induced autophagy in Drosophila by histone methyltransferase G9a
title_sort	epigenetic regulation of starvation-induced autophagy in drosophila by histone methyltransferase g9a
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5f97ee86ce27437db572b832c7291c21
work_keys_str_mv	AT phannguyenthuyan epigeneticregulationofstarvationinducedautophagyindrosophilabyhistonemethyltransferaseg9a AT kouheishimaji epigeneticregulationofstarvationinducedautophagyindrosophilabyhistonemethyltransferaseg9a AT ryotanaka epigeneticregulationofstarvationinducedautophagyindrosophilabyhistonemethyltransferaseg9a AT hidekiyoshida epigeneticregulationofstarvationinducedautophagyindrosophilabyhistonemethyltransferaseg9a AT hiroshikimura epigeneticregulationofstarvationinducedautophagyindrosophilabyhistonemethyltransferaseg9a AT eiichirofukusaki epigeneticregulationofstarvationinducedautophagyindrosophilabyhistonemethyltransferaseg9a AT masamitsuyamaguchi epigeneticregulationofstarvationinducedautophagyindrosophilabyhistonemethyltransferaseg9a
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Epigenetic regulation of starvation-induced autophagy in Drosophila by histone methyltransferase G9a

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