Characterization of ruminal bacteria in grazing Nellore steers

Background: Rumen microorganisms have developed a series of complex interactions, representing one of the best examples of symbiosis between microorganisms in nature. Conventional taxonomic methods based on culture techniques are being replaced by molecular techniques that are faster and more accura...

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Autores principales:	Raphael Barbetta-de-Jesus, Yury T. Granja-Salcedo, Juliana D. Messana, Luciano Takeshi-Kishi, Eliana Gertrudes Macedo-Lemos, Jackson Antonio Marcondes-de-Souza, Telma Teresinha-Berchielli
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2019
Materias:	bacterial diversity cynodon spp firmicutes nellore new generation sequencing steers ruminant ruminal bacteria rumen microorganisms zebu Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/5f99ec139a034d3b86da91b54e63022c
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id	oai:doaj.org-article:5f99ec139a034d3b86da91b54e63022c
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spelling	oai:doaj.org-article:5f99ec139a034d3b86da91b54e63022c2021-12-01T15:53:16ZCharacterization of ruminal bacteria in grazing Nellore steers2256-295810.17533/udea.rccp.v32n4a01https://doaj.org/article/5f99ec139a034d3b86da91b54e63022c2019-11-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/337876https://doaj.org/toc/2256-2958Background: Rumen microorganisms have developed a series of complex interactions, representing one of the best examples of symbiosis between microorganisms in nature. Conventional taxonomic methods based on culture techniques are being replaced by molecular techniques that are faster and more accurate. Objective: To characterize rumen bacterial diversity of Nellore steers grazing on tropical pastures by sequencing the 16S rRNA gene using Illumina sequenctng. Methods: Three rumen-cannulated Nellore steers were used. The liquid and solid fractions of the rumen contents were processed to extract metagenomic DNA, and the VI and V2 hypervariable regions of the 16S rRNA gene were sequenced using Illumina sequencing. Results: A total of 11,407,000 reads of adequate quality were generated, and 812 operational taxonomic units (OTUs) were found at the species level. Twenty-seven phyla were identified, and the predominant phyla were Firmicutes (23%), Bacteroidetes (14%), Proteobacteria (10%), Spirochaetes (9%), Fibrobacteres (7%), Tenericutes (5%), and Actinobacteria (2%), which represented 70% of the total phyla identified in the rumen content. Conclusion: Rumen environment in grazing Nellore steers showed high bacterial diversity, with Firmicutes, Bacteroidetes, Proteobacteria, Spirochaetes, and Fibrobacteres as the predominant phyla.Raphael Barbetta-de-JesusYury T. Granja-SalcedoJuliana D. MessanaLuciano Takeshi-KishiEliana Gertrudes Macedo-LemosJackson Antonio Marcondes-de-SouzaTelma Teresinha-BerchielliUniversidad de Antioquiaarticlebacterial diversitycynodon sppfirmicutesnellorenew generation sequencingsteersruminantruminal bacteriarumen microorganismszebuAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 32, Iss 4, Pp 248-260 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial diversity cynodon spp firmicutes nellore new generation sequencing steers ruminant ruminal bacteria rumen microorganisms zebu Animal culture SF1-1100
spellingShingle	bacterial diversity cynodon spp firmicutes nellore new generation sequencing steers ruminant ruminal bacteria rumen microorganisms zebu Animal culture SF1-1100 Raphael Barbetta-de-Jesus Yury T. Granja-Salcedo Juliana D. Messana Luciano Takeshi-Kishi Eliana Gertrudes Macedo-Lemos Jackson Antonio Marcondes-de-Souza Telma Teresinha-Berchielli Characterization of ruminal bacteria in grazing Nellore steers
description	Background: Rumen microorganisms have developed a series of complex interactions, representing one of the best examples of symbiosis between microorganisms in nature. Conventional taxonomic methods based on culture techniques are being replaced by molecular techniques that are faster and more accurate. Objective: To characterize rumen bacterial diversity of Nellore steers grazing on tropical pastures by sequencing the 16S rRNA gene using Illumina sequenctng. Methods: Three rumen-cannulated Nellore steers were used. The liquid and solid fractions of the rumen contents were processed to extract metagenomic DNA, and the VI and V2 hypervariable regions of the 16S rRNA gene were sequenced using Illumina sequencing. Results: A total of 11,407,000 reads of adequate quality were generated, and 812 operational taxonomic units (OTUs) were found at the species level. Twenty-seven phyla were identified, and the predominant phyla were Firmicutes (23%), Bacteroidetes (14%), Proteobacteria (10%), Spirochaetes (9%), Fibrobacteres (7%), Tenericutes (5%), and Actinobacteria (2%), which represented 70% of the total phyla identified in the rumen content. Conclusion: Rumen environment in grazing Nellore steers showed high bacterial diversity, with Firmicutes, Bacteroidetes, Proteobacteria, Spirochaetes, and Fibrobacteres as the predominant phyla.
format	article
author	Raphael Barbetta-de-Jesus Yury T. Granja-Salcedo Juliana D. Messana Luciano Takeshi-Kishi Eliana Gertrudes Macedo-Lemos Jackson Antonio Marcondes-de-Souza Telma Teresinha-Berchielli
author_facet	Raphael Barbetta-de-Jesus Yury T. Granja-Salcedo Juliana D. Messana Luciano Takeshi-Kishi Eliana Gertrudes Macedo-Lemos Jackson Antonio Marcondes-de-Souza Telma Teresinha-Berchielli
author_sort	Raphael Barbetta-de-Jesus
title	Characterization of ruminal bacteria in grazing Nellore steers
title_short	Characterization of ruminal bacteria in grazing Nellore steers
title_full	Characterization of ruminal bacteria in grazing Nellore steers
title_fullStr	Characterization of ruminal bacteria in grazing Nellore steers
title_full_unstemmed	Characterization of ruminal bacteria in grazing Nellore steers
title_sort	characterization of ruminal bacteria in grazing nellore steers
publisher	Universidad de Antioquia
publishDate	2019
url	https://doaj.org/article/5f99ec139a034d3b86da91b54e63022c
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Characterization of ruminal bacteria in grazing Nellore steers

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