Acute Toxicity of Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Ethanol Extract on Zebrafish (Danio rerio) Embryo In Vivo

Keladi tikus (Typhonium flagelliforme (Lodd.) Blume) is an Indonesian medicinal plant that has various pharmacological properties. Zebrafish (Danio rerio) has been proposed as a model that can bridge the gap between cell assays and rodent assays. Evaluation of the toxic effects of natural products u...

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Autores principales:	Fajar Fakri, Loly Subhiaty Idrus, Maria Alexandra Iskandar, Indra Wibowo, I Ketut Adnyana
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2020
Materias:	keladi tikus ethanol extract zebrafish embryo acute toxicity Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/5fa3bb8d00384861941d38db118ba8c0
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spelling	oai:doaj.org-article:5fa3bb8d00384861941d38db118ba8c02021-11-15T06:08:47ZAcute Toxicity of Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Ethanol Extract on Zebrafish (Danio rerio) Embryo In Vivo2338-94272338-948610.22146/ijp.1121https://doaj.org/article/5fa3bb8d00384861941d38db118ba8c02020-12-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/1121https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Keladi tikus (Typhonium flagelliforme (Lodd.) Blume) is an Indonesian medicinal plant that has various pharmacological properties. Zebrafish (Danio rerio) has been proposed as a model that can bridge the gap between cell assays and rodent assays. Evaluation of the toxic effects of natural products using the Zebrafish model can be assessed starting from the blastula stage of embryonic development. This study aims to investigate the potential acute toxicity effect of keladi tikus-ethanol extract (KTEE) using zebrafish embryos. A static non-replacement regime for acute toxicity testing was used. Wild-type zebrafish embryos were exposed to various concentrations of KTEE (50, 100, 200, 400, 800, 1600 µg/mL) starting at 6 hours post-fertilization (hpf) until 96 hpf. The results showed that the survival rate of zebrafish embryos decreased as the concentration of the test extract increased. The LC50 values of KTEE were 494.553 µg/mL at 96 hpf and 555.787 µg/mL at 72 hpf. Embryotoxicity effect of KTEE includes hatching delays and decreased heartrate on zebrafish embryos, especially at high concentrations. KTEE also caused abnormalities in embryo morphology, including pericardial edema, jaw and tail deformity.Fajar FakriLoly Subhiaty IdrusMaria Alexandra IskandarIndra WibowoI Ketut AdnyanaUniversitas Gadjah Madaarticlekeladi tikusethanol extractzebrafish embryoacute toxicityPharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 297–304-297–304 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	keladi tikus ethanol extract zebrafish embryo acute toxicity Pharmacy and materia medica RS1-441
spellingShingle	keladi tikus ethanol extract zebrafish embryo acute toxicity Pharmacy and materia medica RS1-441 Fajar Fakri Loly Subhiaty Idrus Maria Alexandra Iskandar Indra Wibowo I Ketut Adnyana Acute Toxicity of Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Ethanol Extract on Zebrafish (Danio rerio) Embryo In Vivo
description	Keladi tikus (Typhonium flagelliforme (Lodd.) Blume) is an Indonesian medicinal plant that has various pharmacological properties. Zebrafish (Danio rerio) has been proposed as a model that can bridge the gap between cell assays and rodent assays. Evaluation of the toxic effects of natural products using the Zebrafish model can be assessed starting from the blastula stage of embryonic development. This study aims to investigate the potential acute toxicity effect of keladi tikus-ethanol extract (KTEE) using zebrafish embryos. A static non-replacement regime for acute toxicity testing was used. Wild-type zebrafish embryos were exposed to various concentrations of KTEE (50, 100, 200, 400, 800, 1600 µg/mL) starting at 6 hours post-fertilization (hpf) until 96 hpf. The results showed that the survival rate of zebrafish embryos decreased as the concentration of the test extract increased. The LC50 values of KTEE were 494.553 µg/mL at 96 hpf and 555.787 µg/mL at 72 hpf. Embryotoxicity effect of KTEE includes hatching delays and decreased heartrate on zebrafish embryos, especially at high concentrations. KTEE also caused abnormalities in embryo morphology, including pericardial edema, jaw and tail deformity.
format	article
author	Fajar Fakri Loly Subhiaty Idrus Maria Alexandra Iskandar Indra Wibowo I Ketut Adnyana
author_facet	Fajar Fakri Loly Subhiaty Idrus Maria Alexandra Iskandar Indra Wibowo I Ketut Adnyana
author_sort	Fajar Fakri
title	Acute Toxicity of Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Ethanol Extract on Zebrafish (Danio rerio) Embryo In Vivo
title_short	Acute Toxicity of Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Ethanol Extract on Zebrafish (Danio rerio) Embryo In Vivo
title_full	Acute Toxicity of Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Ethanol Extract on Zebrafish (Danio rerio) Embryo In Vivo
title_fullStr	Acute Toxicity of Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Ethanol Extract on Zebrafish (Danio rerio) Embryo In Vivo
title_full_unstemmed	Acute Toxicity of Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Ethanol Extract on Zebrafish (Danio rerio) Embryo In Vivo
title_sort	acute toxicity of keladi tikus (typhonium flagelliforme (lodd.) blume) ethanol extract on zebrafish (danio rerio) embryo in vivo
publisher	Universitas Gadjah Mada
publishDate	2020
url	https://doaj.org/article/5fa3bb8d00384861941d38db118ba8c0
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Acute Toxicity of Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Ethanol Extract on Zebrafish (Danio rerio) Embryo In Vivo

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