An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host

Abstract Endophytes, both of bacterial and fungal origin, are ubiquitously present in all plants. While their origin and evolution are enigmatic, there is burgeoning literature on their role in promoting growth and stress responses in their hosts. We demonstrate that a salt-tolerant endophyte isolat...

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Autores principales:	Megha Hastantram Sampangi-Ramaiah, Jagadheesh, Prajjal Dey, Shridhar Jambagi, M. M. Vasantha Kumari, Ralf Oelmüller, Karaba N. Nataraja, Kundapura Venkataramana Ravishankar, G. Ravikanth, R. Uma Shaanker
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5fa57e1c34534ec1a69689fd42bc7b32
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spelling	oai:doaj.org-article:5fa57e1c34534ec1a69689fd42bc7b322021-12-02T14:28:21ZAn endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host10.1038/s41598-020-59998-x2045-2322https://doaj.org/article/5fa57e1c34534ec1a69689fd42bc7b322020-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-59998-xhttps://doaj.org/toc/2045-2322Abstract Endophytes, both of bacterial and fungal origin, are ubiquitously present in all plants. While their origin and evolution are enigmatic, there is burgeoning literature on their role in promoting growth and stress responses in their hosts. We demonstrate that a salt-tolerant endophyte isolated from salt-adapted Pokkali rice, a Fusarium sp., colonizes the salt-sensitive rice variety IR-64, promotes its growth under salt stress and confers salinity stress tolerance to its host. Physiological parameters, such as assimilation rate and chlorophyll stability index were higher in the colonized plants. Comparative transcriptome analysis revealed 1348 up-regulated and 1078 down-regulated genes in plants colonized by the endophyte. Analysis of the regulated genes by MapMan and interaction network programs showed that they are involved in both abiotic and biotic stress tolerance, and code for proteins involved in signal perception (leucine-rich repeat proteins, receptor-like kinases) and transduction (Ca2+ and calmodulin-binding proteins), transcription factors, secondary metabolism and oxidative stress scavenging. For nine genes, the data were validated by qPCR analysis in both roots and shoots. Taken together, these results show that salt-adapted Pokkali rice varieties are powerful sources for the identification of novel endophytes, which can be used to confer salinity tolerance to agriculturally important, but salt-sensitive rice varieties.Megha Hastantram Sampangi-RamaiahJagadheeshPrajjal DeyShridhar JambagiM. M. Vasantha KumariRalf OelmüllerKaraba N. NatarajaKundapura Venkataramana RavishankarG. RavikanthR. Uma ShaankerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Megha Hastantram Sampangi-Ramaiah Jagadheesh Prajjal Dey Shridhar Jambagi M. M. Vasantha Kumari Ralf Oelmüller Karaba N. Nataraja Kundapura Venkataramana Ravishankar G. Ravikanth R. Uma Shaanker An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host
description	Abstract Endophytes, both of bacterial and fungal origin, are ubiquitously present in all plants. While their origin and evolution are enigmatic, there is burgeoning literature on their role in promoting growth and stress responses in their hosts. We demonstrate that a salt-tolerant endophyte isolated from salt-adapted Pokkali rice, a Fusarium sp., colonizes the salt-sensitive rice variety IR-64, promotes its growth under salt stress and confers salinity stress tolerance to its host. Physiological parameters, such as assimilation rate and chlorophyll stability index were higher in the colonized plants. Comparative transcriptome analysis revealed 1348 up-regulated and 1078 down-regulated genes in plants colonized by the endophyte. Analysis of the regulated genes by MapMan and interaction network programs showed that they are involved in both abiotic and biotic stress tolerance, and code for proteins involved in signal perception (leucine-rich repeat proteins, receptor-like kinases) and transduction (Ca2+ and calmodulin-binding proteins), transcription factors, secondary metabolism and oxidative stress scavenging. For nine genes, the data were validated by qPCR analysis in both roots and shoots. Taken together, these results show that salt-adapted Pokkali rice varieties are powerful sources for the identification of novel endophytes, which can be used to confer salinity tolerance to agriculturally important, but salt-sensitive rice varieties.
format	article
author	Megha Hastantram Sampangi-Ramaiah Jagadheesh Prajjal Dey Shridhar Jambagi M. M. Vasantha Kumari Ralf Oelmüller Karaba N. Nataraja Kundapura Venkataramana Ravishankar G. Ravikanth R. Uma Shaanker
author_facet	Megha Hastantram Sampangi-Ramaiah Jagadheesh Prajjal Dey Shridhar Jambagi M. M. Vasantha Kumari Ralf Oelmüller Karaba N. Nataraja Kundapura Venkataramana Ravishankar G. Ravikanth R. Uma Shaanker
author_sort	Megha Hastantram Sampangi-Ramaiah
title	An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host
title_short	An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host
title_full	An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host
title_fullStr	An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host
title_full_unstemmed	An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host
title_sort	endophyte from salt-adapted pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5fa57e1c34534ec1a69689fd42bc7b32
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An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host

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