Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells.

Digital holographic microscopy (DHM) has emerged as a powerful non-invasive tool for cell analysis. It has the capacity to analyse multiple parameters simultaneously, such as cell- number, confluence and phase volume. This is done while cells are still adhered and growing in their culture flask. The...

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Autores principales:	Maria Falck Miniotis, Anthonny Mukwaya, Anette Gjörloff Wingren
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5fa627514e6e4fd69e0dad170d518119
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spelling	oai:doaj.org-article:5fa627514e6e4fd69e0dad170d5181192021-11-25T06:01:06ZDigital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells.1932-620310.1371/journal.pone.0106546https://doaj.org/article/5fa627514e6e4fd69e0dad170d5181192014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0106546https://doaj.org/toc/1932-6203Digital holographic microscopy (DHM) has emerged as a powerful non-invasive tool for cell analysis. It has the capacity to analyse multiple parameters simultaneously, such as cell- number, confluence and phase volume. This is done while cells are still adhered and growing in their culture flask. The aim of this study was to investigate whether DHM was able to monitor drug-induced cell cycle arrest in cultured cells and thus provide a non-disruptive alternative to flow cytometry. DHM parameters from G1 and G2/M cell cycle arrested L929 mouse fibroblast cells were collected. Cell cycle arrest was verified with flow cytometry. This study shows that DHM is able to monitor phase volume changes corresponding to either a G1 or G2/M cell cycle arrest. G1-phase arrest with staurosporine correlated with a decrease in the average cell phase volume and G2/M-phase arrest with colcemid and etoposide correlated with an increase in the average cell phase volume. Importantly, DHM analysis of average cell phase volume was of comparable accuracy to flow cytometric measurement of cell cycle phase distribution as recorded following dose-dependent treatment with etoposide. Average cell phase volume changes in response to treatment with cell cycle arresting compounds could therefore be used as a DHM marker for monitoring cell cycle arrest in cultured mammalian cells.Maria Falck MiniotisAnthonny MukwayaAnette Gjörloff WingrenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e106546 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Falck Miniotis Anthonny Mukwaya Anette Gjörloff Wingren Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells.
description	Digital holographic microscopy (DHM) has emerged as a powerful non-invasive tool for cell analysis. It has the capacity to analyse multiple parameters simultaneously, such as cell- number, confluence and phase volume. This is done while cells are still adhered and growing in their culture flask. The aim of this study was to investigate whether DHM was able to monitor drug-induced cell cycle arrest in cultured cells and thus provide a non-disruptive alternative to flow cytometry. DHM parameters from G1 and G2/M cell cycle arrested L929 mouse fibroblast cells were collected. Cell cycle arrest was verified with flow cytometry. This study shows that DHM is able to monitor phase volume changes corresponding to either a G1 or G2/M cell cycle arrest. G1-phase arrest with staurosporine correlated with a decrease in the average cell phase volume and G2/M-phase arrest with colcemid and etoposide correlated with an increase in the average cell phase volume. Importantly, DHM analysis of average cell phase volume was of comparable accuracy to flow cytometric measurement of cell cycle phase distribution as recorded following dose-dependent treatment with etoposide. Average cell phase volume changes in response to treatment with cell cycle arresting compounds could therefore be used as a DHM marker for monitoring cell cycle arrest in cultured mammalian cells.
format	article
author	Maria Falck Miniotis Anthonny Mukwaya Anette Gjörloff Wingren
author_facet	Maria Falck Miniotis Anthonny Mukwaya Anette Gjörloff Wingren
author_sort	Maria Falck Miniotis
title	Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells.
title_short	Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells.
title_full	Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells.
title_fullStr	Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells.
title_full_unstemmed	Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells.
title_sort	digital holographic microscopy for non-invasive monitoring of cell cycle arrest in l929 cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/5fa627514e6e4fd69e0dad170d518119
work_keys_str_mv	AT mariafalckminiotis digitalholographicmicroscopyfornoninvasivemonitoringofcellcyclearrestinl929cells AT anthonnymukwaya digitalholographicmicroscopyfornoninvasivemonitoringofcellcyclearrestinl929cells AT anettegjorloffwingren digitalholographicmicroscopyfornoninvasivemonitoringofcellcyclearrestinl929cells
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Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells.

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