A generic standard additions based method to determine endogenous analyte concentrations by immunoassays to overcome complex biological matrix interference

Abstract We describe a novel generic method to derive the unknown endogenous concentrations of analyte within complex biological matrices (e.g. serum or plasma) based upon the relationship between the immunoassay signal response of a biological test sample spiked with known analyte concentrations an...

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Autores principales:	Susan Pang, Simon Cowen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5fa6428a780b43e0b7fe2236ea8d295f
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spelling	oai:doaj.org-article:5fa6428a780b43e0b7fe2236ea8d295f2021-12-02T15:06:07ZA generic standard additions based method to determine endogenous analyte concentrations by immunoassays to overcome complex biological matrix interference10.1038/s41598-017-17823-y2045-2322https://doaj.org/article/5fa6428a780b43e0b7fe2236ea8d295f2017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-17823-yhttps://doaj.org/toc/2045-2322Abstract We describe a novel generic method to derive the unknown endogenous concentrations of analyte within complex biological matrices (e.g. serum or plasma) based upon the relationship between the immunoassay signal response of a biological test sample spiked with known analyte concentrations and the log transformed estimated total concentration. If the estimated total analyte concentration is correct, a portion of the sigmoid on a log-log plot is very close to linear, allowing the unknown endogenous concentration to be estimated using a numerical method. This approach obviates conventional relative quantification using an internal standard curve and need for calibrant diluent, and takes into account the individual matrix interference on the immunoassay by spiking the test sample itself. This technique is based on standard additions for chemical analytes. Unknown endogenous analyte concentrations within even 2-fold diluted human plasma may be determined reliably using as few as four reaction wells.Susan PangSimon CowenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Susan Pang Simon Cowen A generic standard additions based method to determine endogenous analyte concentrations by immunoassays to overcome complex biological matrix interference
description	Abstract We describe a novel generic method to derive the unknown endogenous concentrations of analyte within complex biological matrices (e.g. serum or plasma) based upon the relationship between the immunoassay signal response of a biological test sample spiked with known analyte concentrations and the log transformed estimated total concentration. If the estimated total analyte concentration is correct, a portion of the sigmoid on a log-log plot is very close to linear, allowing the unknown endogenous concentration to be estimated using a numerical method. This approach obviates conventional relative quantification using an internal standard curve and need for calibrant diluent, and takes into account the individual matrix interference on the immunoassay by spiking the test sample itself. This technique is based on standard additions for chemical analytes. Unknown endogenous analyte concentrations within even 2-fold diluted human plasma may be determined reliably using as few as four reaction wells.
format	article
author	Susan Pang Simon Cowen
author_facet	Susan Pang Simon Cowen
author_sort	Susan Pang
title	A generic standard additions based method to determine endogenous analyte concentrations by immunoassays to overcome complex biological matrix interference
title_short	A generic standard additions based method to determine endogenous analyte concentrations by immunoassays to overcome complex biological matrix interference
title_full	A generic standard additions based method to determine endogenous analyte concentrations by immunoassays to overcome complex biological matrix interference
title_fullStr	A generic standard additions based method to determine endogenous analyte concentrations by immunoassays to overcome complex biological matrix interference
title_full_unstemmed	A generic standard additions based method to determine endogenous analyte concentrations by immunoassays to overcome complex biological matrix interference
title_sort	generic standard additions based method to determine endogenous analyte concentrations by immunoassays to overcome complex biological matrix interference
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5fa6428a780b43e0b7fe2236ea8d295f
work_keys_str_mv	AT susanpang agenericstandardadditionsbasedmethodtodetermineendogenousanalyteconcentrationsbyimmunoassaystoovercomecomplexbiologicalmatrixinterference AT simoncowen agenericstandardadditionsbasedmethodtodetermineendogenousanalyteconcentrationsbyimmunoassaystoovercomecomplexbiologicalmatrixinterference AT susanpang genericstandardadditionsbasedmethodtodetermineendogenousanalyteconcentrationsbyimmunoassaystoovercomecomplexbiologicalmatrixinterference AT simoncowen genericstandardadditionsbasedmethodtodetermineendogenousanalyteconcentrationsbyimmunoassaystoovercomecomplexbiologicalmatrixinterference
_version_	1718388585253568512

A generic standard additions based method to determine endogenous analyte concentrations by immunoassays to overcome complex biological matrix interference

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