Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae

Abstract Olfactory sensing is generally organized into groups of similarly sensing olfactory receptor neurons converging into their corresponding glomerulus, which is thought to behave as a uniform functional unit. It is however unclear to which degree axons within a glomerulus show identical activi...

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Autores principales:	Rodi Topci, Mihai Alevra, Erik H. U. Rauf, Daniëlle de Jong-Bolm
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5fab873b6cf848dc91439eac1bde3e36
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spelling	oai:doaj.org-article:5fab873b6cf848dc91439eac1bde3e362021-12-02T18:34:20ZResolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae10.1038/s41598-021-93677-92045-2322https://doaj.org/article/5fab873b6cf848dc91439eac1bde3e362021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93677-9https://doaj.org/toc/2045-2322Abstract Olfactory sensing is generally organized into groups of similarly sensing olfactory receptor neurons converging into their corresponding glomerulus, which is thought to behave as a uniform functional unit. It is however unclear to which degree axons within a glomerulus show identical activity, how many converge into a glomerulus, and to answer these questions, how it is possible to visually separate them in live imaging. Here we investigate activity of olfactory receptor neurons and their axon terminals throughout olfactory glomeruli using electrophysiological recordings and rapid 4D calcium imaging. While single olfactory receptor neurons responsive to the same odor stimulus show a diversity of responses in terms of sensitivity and spontaneous firing rate on the level of the somata, their pre-synaptic calcium activity in the glomerulus is homogeneous. In addition, we could not observe the correlated spontaneous calcium activity that is found on the post-synaptic side throughout mitral cell dendrites and has been used in activity correlation imaging. However, it is possible to induce spatio-temporal presynaptic response inhomogeneities by applying trains of olfactory stimuli with varying amino acid concentrations. Automated region-of-interest detection and correlation analysis then visually distinguishes at least two axon subgroups per glomerulus that differ in odor sensitivity.Rodi TopciMihai AlevraErik H. U. RaufDaniëlle de Jong-BolmNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rodi Topci Mihai Alevra Erik H. U. Rauf Daniëlle de Jong-Bolm Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae
description	Abstract Olfactory sensing is generally organized into groups of similarly sensing olfactory receptor neurons converging into their corresponding glomerulus, which is thought to behave as a uniform functional unit. It is however unclear to which degree axons within a glomerulus show identical activity, how many converge into a glomerulus, and to answer these questions, how it is possible to visually separate them in live imaging. Here we investigate activity of olfactory receptor neurons and their axon terminals throughout olfactory glomeruli using electrophysiological recordings and rapid 4D calcium imaging. While single olfactory receptor neurons responsive to the same odor stimulus show a diversity of responses in terms of sensitivity and spontaneous firing rate on the level of the somata, their pre-synaptic calcium activity in the glomerulus is homogeneous. In addition, we could not observe the correlated spontaneous calcium activity that is found on the post-synaptic side throughout mitral cell dendrites and has been used in activity correlation imaging. However, it is possible to induce spatio-temporal presynaptic response inhomogeneities by applying trains of olfactory stimuli with varying amino acid concentrations. Automated region-of-interest detection and correlation analysis then visually distinguishes at least two axon subgroups per glomerulus that differ in odor sensitivity.
format	article
author	Rodi Topci Mihai Alevra Erik H. U. Rauf Daniëlle de Jong-Bolm
author_facet	Rodi Topci Mihai Alevra Erik H. U. Rauf Daniëlle de Jong-Bolm
author_sort	Rodi Topci
title	Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae
title_short	Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae
title_full	Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae
title_fullStr	Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae
title_full_unstemmed	Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae
title_sort	resolving different presynaptic activity patterns within single olfactory glomeruli of xenopus laevis larvae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5fab873b6cf848dc91439eac1bde3e36
work_keys_str_mv	AT roditopci resolvingdifferentpresynapticactivitypatternswithinsingleolfactoryglomeruliofxenopuslaevislarvae AT mihaialevra resolvingdifferentpresynapticactivitypatternswithinsingleolfactoryglomeruliofxenopuslaevislarvae AT erikhurauf resolvingdifferentpresynapticactivitypatternswithinsingleolfactoryglomeruliofxenopuslaevislarvae AT danielledejongbolm resolvingdifferentpresynapticactivitypatternswithinsingleolfactoryglomeruliofxenopuslaevislarvae
_version_	1718377862330843136

Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae

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