Serotonin 5-HT2A receptor activity mediates adipocyte differentiation through control of adipogenic gene expression

Abstract Serotonin 5-HT2 receptors are expressed in many tissues and play important roles in biological processes. Although the 5-HT2A receptor is primarily known for its role in central nervous system, it is also expressed in peripheral tissues. We have found that 5-HT2A receptor antagonists inhibi...

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Autores principales:	Bangning Yu, Diana M. Battaglia, Timothy P. Foster, Charles D. Nichols
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5fb84b6d50004e4bbf63c2cb1818b2b0
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spelling	oai:doaj.org-article:5fb84b6d50004e4bbf63c2cb1818b2b02021-12-02T19:16:14ZSerotonin 5-HT2A receptor activity mediates adipocyte differentiation through control of adipogenic gene expression10.1038/s41598-021-98970-12045-2322https://doaj.org/article/5fb84b6d50004e4bbf63c2cb1818b2b02021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98970-1https://doaj.org/toc/2045-2322Abstract Serotonin 5-HT2 receptors are expressed in many tissues and play important roles in biological processes. Although the 5-HT2A receptor is primarily known for its role in central nervous system, it is also expressed in peripheral tissues. We have found that 5-HT2A receptor antagonists inhibit human subcutaneous primary adipocyte differentiation. We also show that siRNA knockdown of the 5-HT2A receptor blocks differentiation. Using gene expression analysis in combination with receptor antagonists we found that activity of 5-HT2A receptors is necessary very early in the differentiation process to mediate expression of adipogenic genes, including peroxisome proliferator-activated receptor gamma (ppar-γ), adipocyte protein 2 (aP2), adiponectin, and serine/threonine-protein kinase 1 (sgk1). We show here for the first time that 5-HT2A receptor activity is necessary for differentiation of human primary subcutaneous preadipocytes to adipocytes, and that 5-HT2A receptor activity mediates key genes related to adipogenesis during this process. Importantly, this work contributes to a greater understanding of the adipocyte differentiation process, as well as to the role of 5-HT2A receptors in peripheral tissues, and may be relevant to the development of novel therapeutic strategies targeting this receptor for the treatment of obesity related diseases.Bangning YuDiana M. BattagliaTimothy P. FosterCharles D. NicholsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bangning Yu Diana M. Battaglia Timothy P. Foster Charles D. Nichols Serotonin 5-HT2A receptor activity mediates adipocyte differentiation through control of adipogenic gene expression
description	Abstract Serotonin 5-HT2 receptors are expressed in many tissues and play important roles in biological processes. Although the 5-HT2A receptor is primarily known for its role in central nervous system, it is also expressed in peripheral tissues. We have found that 5-HT2A receptor antagonists inhibit human subcutaneous primary adipocyte differentiation. We also show that siRNA knockdown of the 5-HT2A receptor blocks differentiation. Using gene expression analysis in combination with receptor antagonists we found that activity of 5-HT2A receptors is necessary very early in the differentiation process to mediate expression of adipogenic genes, including peroxisome proliferator-activated receptor gamma (ppar-γ), adipocyte protein 2 (aP2), adiponectin, and serine/threonine-protein kinase 1 (sgk1). We show here for the first time that 5-HT2A receptor activity is necessary for differentiation of human primary subcutaneous preadipocytes to adipocytes, and that 5-HT2A receptor activity mediates key genes related to adipogenesis during this process. Importantly, this work contributes to a greater understanding of the adipocyte differentiation process, as well as to the role of 5-HT2A receptors in peripheral tissues, and may be relevant to the development of novel therapeutic strategies targeting this receptor for the treatment of obesity related diseases.
format	article
author	Bangning Yu Diana M. Battaglia Timothy P. Foster Charles D. Nichols
author_facet	Bangning Yu Diana M. Battaglia Timothy P. Foster Charles D. Nichols
author_sort	Bangning Yu
title	Serotonin 5-HT2A receptor activity mediates adipocyte differentiation through control of adipogenic gene expression
title_short	Serotonin 5-HT2A receptor activity mediates adipocyte differentiation through control of adipogenic gene expression
title_full	Serotonin 5-HT2A receptor activity mediates adipocyte differentiation through control of adipogenic gene expression
title_fullStr	Serotonin 5-HT2A receptor activity mediates adipocyte differentiation through control of adipogenic gene expression
title_full_unstemmed	Serotonin 5-HT2A receptor activity mediates adipocyte differentiation through control of adipogenic gene expression
title_sort	serotonin 5-ht2a receptor activity mediates adipocyte differentiation through control of adipogenic gene expression
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5fb84b6d50004e4bbf63c2cb1818b2b0
work_keys_str_mv	AT bangningyu serotonin5ht2areceptoractivitymediatesadipocytedifferentiationthroughcontrolofadipogenicgeneexpression AT dianambattaglia serotonin5ht2areceptoractivitymediatesadipocytedifferentiationthroughcontrolofadipogenicgeneexpression AT timothypfoster serotonin5ht2areceptoractivitymediatesadipocytedifferentiationthroughcontrolofadipogenicgeneexpression AT charlesdnichols serotonin5ht2areceptoractivitymediatesadipocytedifferentiationthroughcontrolofadipogenicgeneexpression
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Serotonin 5-HT2A receptor activity mediates adipocyte differentiation through control of adipogenic gene expression

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