Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells

Rosa Di Liddo,1,2 Paola Aguiari,3 Silvia Barbon,1,2 Thomas Bertalot,1 Amit Mandoli,1 Alessia Tasso,1 Sandra Schrenk,1 Laura Iop,3 Alessandro Gandaglia,3 Pier Paolo Parnigotto,2 Maria Teresa Conconi,1,2 Gino Gerosa31Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 2Fou...

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Autores principales:	Di Liddo R, Aguiari P, Barbon S, Bertalot T, Mandoli A, Tasso A, Schrenk S, Iop L, Gandaglia A, Parnigotto PP, Conconi MT, Gerosa G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Blood-derived multipotent cells Self-repopulation potential Guided tissue engineering TriCol decellularization procedure ECM nanostructure signaling Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5fbb18f062a84d2ebbca236c043fba16
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spelling	oai:doaj.org-article:5fbb18f062a84d2ebbca236c043fba162021-12-02T02:45:55ZNanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells1178-2013https://doaj.org/article/5fbb18f062a84d2ebbca236c043fba162016-10-01T00:00:00Zhttps://www.dovepress.com/nanopatterned-acellular-valve-conduits-drive-the-commitment-of-blood-d-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Rosa Di Liddo,1,2 Paola Aguiari,3 Silvia Barbon,1,2 Thomas Bertalot,1 Amit Mandoli,1 Alessia Tasso,1 Sandra Schrenk,1 Laura Iop,3 Alessandro Gandaglia,3 Pier Paolo Parnigotto,2 Maria Teresa Conconi,1,2 Gino Gerosa31Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 2Foundation for Biology and Regenerative Medicine, Tissue Engineering and Signaling ONLUS, 3Department of Cardiac, Thoracic and Vascular Sciences, University of Padova, Padova, Italy Abstract: Considerable progress has been made in recent years toward elucidating the correlation among nanoscale topography, mechanical properties, and biological behavior of cardiac valve substitutes. Porcine TriCol scaffolds are promising valve tissue engineering matrices with demonstrated self-repopulation potentiality. In order to define an in vitro model for investigating the influence of extracellular matrix signaling on the growth pattern of colonizing blood-derived cells, we cultured circulating multipotent cells (CMC) on acellular aortic (AVL) and pulmonary (PVL) valve conduits prepared with TriCol method and under no-flow condition. Isolated by our group from Vietnamese pigs before heart valve prosthetic implantation, porcine CMC revealed high proliferative abilities, three-lineage differentiative potential, and distinct hematopoietic/endothelial and mesenchymal properties. Their interaction with valve extracellular matrix nanostructures boosted differential messenger RNA expression pattern and morphologic features on AVL compared to PVL, while promoting on both matrices the commitment to valvular and endothelial cell-like phenotypes. Based on their origin from peripheral blood, porcine CMC are hypothesized in vivo to exert a pivotal role to homeostatically replenish valve cells and contribute to hetero- or allograft colonization. Furthermore, due to their high responsivity to extracellular matrix nanostructure signaling, porcine CMC could be useful for a preliminary evaluation of heart valve prosthetic functionality. Keywords: blood-derived multipotent cells, self-repopulation potential, guided tissue engineering, TriCol decellularization procedure, ECM nanostructure signalingDi Liddo RAguiari PBarbon SBertalot TMandoli ATasso ASchrenk SIop LGandaglia AParnigotto PPConconi MTGerosa GDove Medical PressarticleBlood-derived multipotent cellsSelf-repopulation potentialGuided tissue engineeringTriCol decellularization procedureECM nanostructure signalingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5041-5055 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Blood-derived multipotent cells Self-repopulation potential Guided tissue engineering TriCol decellularization procedure ECM nanostructure signaling Medicine (General) R5-920
spellingShingle	Blood-derived multipotent cells Self-repopulation potential Guided tissue engineering TriCol decellularization procedure ECM nanostructure signaling Medicine (General) R5-920 Di Liddo R Aguiari P Barbon S Bertalot T Mandoli A Tasso A Schrenk S Iop L Gandaglia A Parnigotto PP Conconi MT Gerosa G Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
description	Rosa Di Liddo,1,2 Paola Aguiari,3 Silvia Barbon,1,2 Thomas Bertalot,1 Amit Mandoli,1 Alessia Tasso,1 Sandra Schrenk,1 Laura Iop,3 Alessandro Gandaglia,3 Pier Paolo Parnigotto,2 Maria Teresa Conconi,1,2 Gino Gerosa31Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 2Foundation for Biology and Regenerative Medicine, Tissue Engineering and Signaling ONLUS, 3Department of Cardiac, Thoracic and Vascular Sciences, University of Padova, Padova, Italy Abstract: Considerable progress has been made in recent years toward elucidating the correlation among nanoscale topography, mechanical properties, and biological behavior of cardiac valve substitutes. Porcine TriCol scaffolds are promising valve tissue engineering matrices with demonstrated self-repopulation potentiality. In order to define an in vitro model for investigating the influence of extracellular matrix signaling on the growth pattern of colonizing blood-derived cells, we cultured circulating multipotent cells (CMC) on acellular aortic (AVL) and pulmonary (PVL) valve conduits prepared with TriCol method and under no-flow condition. Isolated by our group from Vietnamese pigs before heart valve prosthetic implantation, porcine CMC revealed high proliferative abilities, three-lineage differentiative potential, and distinct hematopoietic/endothelial and mesenchymal properties. Their interaction with valve extracellular matrix nanostructures boosted differential messenger RNA expression pattern and morphologic features on AVL compared to PVL, while promoting on both matrices the commitment to valvular and endothelial cell-like phenotypes. Based on their origin from peripheral blood, porcine CMC are hypothesized in vivo to exert a pivotal role to homeostatically replenish valve cells and contribute to hetero- or allograft colonization. Furthermore, due to their high responsivity to extracellular matrix nanostructure signaling, porcine CMC could be useful for a preliminary evaluation of heart valve prosthetic functionality. Keywords: blood-derived multipotent cells, self-repopulation potential, guided tissue engineering, TriCol decellularization procedure, ECM nanostructure signaling
format	article
author	Di Liddo R Aguiari P Barbon S Bertalot T Mandoli A Tasso A Schrenk S Iop L Gandaglia A Parnigotto PP Conconi MT Gerosa G
author_facet	Di Liddo R Aguiari P Barbon S Bertalot T Mandoli A Tasso A Schrenk S Iop L Gandaglia A Parnigotto PP Conconi MT Gerosa G
author_sort	Di Liddo R
title	Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
title_short	Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
title_full	Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
title_fullStr	Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
title_full_unstemmed	Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
title_sort	nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/5fbb18f062a84d2ebbca236c043fba16
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Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells

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