Characteristics and toxicity assessment of electrospun gelatin/PCL nanofibrous scaffold loaded with graphene in vitro and in vivo

Xi Chen,* Bei Feng,* Di-Qi Zhu, Yi-Wei Chen, Wei Ji, Tian-Ji Ji, Fen LiDepartment of Cardiology, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiaotong University, Shanghai, People’s Republic of China*These authors contributed equally to this workBackground: El...

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Autores principales:	Chen X, Feng B, Zhu DQ, Chen YW, Ji W, Ji TJ, Li F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	gelatin polycaprolactone graphene electrical conductivity toxicity atrioventricular block Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5fbc2331a389422f9f7c0c649e677d65
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spelling	oai:doaj.org-article:5fbc2331a389422f9f7c0c649e677d652021-12-02T03:16:17ZCharacteristics and toxicity assessment of electrospun gelatin/PCL nanofibrous scaffold loaded with graphene in vitro and in vivo1178-2013https://doaj.org/article/5fbc2331a389422f9f7c0c649e677d652019-05-01T00:00:00Zhttps://www.dovepress.com/characteristics-and-toxicity-assessment-of-electrospun-gelatinpcl-nano-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xi Chen,* Bei Feng,* Di-Qi Zhu, Yi-Wei Chen, Wei Ji, Tian-Ji Ji, Fen LiDepartment of Cardiology, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiaotong University, Shanghai, People’s Republic of China*These authors contributed equally to this workBackground: Electrospun gelatin/polycaprolactone (Gt/PCL) nanofibrous scaffolds loaded with graphene are novel nanomaterials with the uniquely strong property of electrical conductivity, which have been widely investigated for their potential applications in cardiovascular tissue engineering, including in bypass tracts for atrioventricular block.Purpose: Electrospun Gt/PCL/graphene nanofibrous mats were successfully produced. Scanning electron micrography showed that the fibers with graphene were smooth and homogeneous. In vitro, to determine the biocompatibility of the scaffolds, hybrid scaffolds with different fractions of graphene were seeded with neonatal rat ventricular myocytes. In vivo, Gt/PCL scaffolds with different concentrations of graphene were implanted into rats for 4, 8 and 12 weeks.Results: CCK-8 assays and histopathological staining (including DAPI, cTNT, and CX43) indicated that cells grew and survived well on the hybrid scaffolds if the mass fraction of graphene was lower than 0.5%. After implanting into rats for 4, 8 or 12 weeks, there was no gathering of inflammatory cells around the nanomaterials according to the HE staining results.Conclusion: The results indicate that Gt/PCL nanofibrous scaffolds loaded with graphene have favorable electrical conductivity and biological properties and may be suitable scaffolds for use in the treatment of atrioventricular block. These findings alleviate safety concerns and provide novel insights into the potential applications of Gt/PCL loaded with graphene, offering a solid foundation for comprehensive in vivo studies.Keywords: gelatin, polycaprolactone, graphene, electrical conductivity, toxicity, atrioventricular blockChen XFeng BZhu DQChen YWJi WJi TJLi FDove Medical Pressarticlegelatinpolycaprolactonegrapheneelectrical conductivitytoxicityatrioventricular blockMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3669-3678 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	gelatin polycaprolactone graphene electrical conductivity toxicity atrioventricular block Medicine (General) R5-920
spellingShingle	gelatin polycaprolactone graphene electrical conductivity toxicity atrioventricular block Medicine (General) R5-920 Chen X Feng B Zhu DQ Chen YW Ji W Ji TJ Li F Characteristics and toxicity assessment of electrospun gelatin/PCL nanofibrous scaffold loaded with graphene in vitro and in vivo
description	Xi Chen,* Bei Feng,* Di-Qi Zhu, Yi-Wei Chen, Wei Ji, Tian-Ji Ji, Fen LiDepartment of Cardiology, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiaotong University, Shanghai, People’s Republic of China*These authors contributed equally to this workBackground: Electrospun gelatin/polycaprolactone (Gt/PCL) nanofibrous scaffolds loaded with graphene are novel nanomaterials with the uniquely strong property of electrical conductivity, which have been widely investigated for their potential applications in cardiovascular tissue engineering, including in bypass tracts for atrioventricular block.Purpose: Electrospun Gt/PCL/graphene nanofibrous mats were successfully produced. Scanning electron micrography showed that the fibers with graphene were smooth and homogeneous. In vitro, to determine the biocompatibility of the scaffolds, hybrid scaffolds with different fractions of graphene were seeded with neonatal rat ventricular myocytes. In vivo, Gt/PCL scaffolds with different concentrations of graphene were implanted into rats for 4, 8 and 12 weeks.Results: CCK-8 assays and histopathological staining (including DAPI, cTNT, and CX43) indicated that cells grew and survived well on the hybrid scaffolds if the mass fraction of graphene was lower than 0.5%. After implanting into rats for 4, 8 or 12 weeks, there was no gathering of inflammatory cells around the nanomaterials according to the HE staining results.Conclusion: The results indicate that Gt/PCL nanofibrous scaffolds loaded with graphene have favorable electrical conductivity and biological properties and may be suitable scaffolds for use in the treatment of atrioventricular block. These findings alleviate safety concerns and provide novel insights into the potential applications of Gt/PCL loaded with graphene, offering a solid foundation for comprehensive in vivo studies.Keywords: gelatin, polycaprolactone, graphene, electrical conductivity, toxicity, atrioventricular block
format	article
author	Chen X Feng B Zhu DQ Chen YW Ji W Ji TJ Li F
author_facet	Chen X Feng B Zhu DQ Chen YW Ji W Ji TJ Li F
author_sort	Chen X
title	Characteristics and toxicity assessment of electrospun gelatin/PCL nanofibrous scaffold loaded with graphene in vitro and in vivo
title_short	Characteristics and toxicity assessment of electrospun gelatin/PCL nanofibrous scaffold loaded with graphene in vitro and in vivo
title_full	Characteristics and toxicity assessment of electrospun gelatin/PCL nanofibrous scaffold loaded with graphene in vitro and in vivo
title_fullStr	Characteristics and toxicity assessment of electrospun gelatin/PCL nanofibrous scaffold loaded with graphene in vitro and in vivo
title_full_unstemmed	Characteristics and toxicity assessment of electrospun gelatin/PCL nanofibrous scaffold loaded with graphene in vitro and in vivo
title_sort	characteristics and toxicity assessment of electrospun gelatin/pcl nanofibrous scaffold loaded with graphene in vitro and in vivo
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/5fbc2331a389422f9f7c0c649e677d65
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Characteristics and toxicity assessment of electrospun gelatin/PCL nanofibrous scaffold loaded with graphene in vitro and in vivo

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