Daunorubicin-loaded magnetic nanoparticles of Fe3O4 overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo

Bao-an Chen1, Bin-bin Lai1, Jian Cheng1, Guo-hua Xia1, Feng Gao1, Wen-lin Xu2, Jia-hua Ding1, Chong Gao1, Xin-chen Sun3, Cui-rong Xu1, Wen-ji Chen1, Ning-na Chen1, Li-jie Liu4, Xiao-mao Li5, Xue-mei Wang61Department of Hematology, 3Department of Oncology, the Affiliated Zhongda Hospital, Clinical Me...

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Autores principales:	Bao-an Chen, Bin-bin Lai, Jian Cheng, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2009
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5fca087d48fe4a589756b55ee4308ece
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spelling	oai:doaj.org-article:5fca087d48fe4a589756b55ee4308ece2021-12-02T11:01:36ZDaunorubicin-loaded magnetic nanoparticles of Fe3O4 overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo1176-91141178-2013https://doaj.org/article/5fca087d48fe4a589756b55ee4308ece2009-09-01T00:00:00Zhttp://www.dovepress.com/daunorubicin-loaded-magnetic-nanoparticles-of-fe3o4-overcome-multidrug-a3571https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Bao-an Chen1, Bin-bin Lai1, Jian Cheng1, Guo-hua Xia1, Feng Gao1, Wen-lin Xu2, Jia-hua Ding1, Chong Gao1, Xin-chen Sun3, Cui-rong Xu1, Wen-ji Chen1, Ning-na Chen1, Li-jie Liu4, Xiao-mao Li5, Xue-mei Wang61Department of Hematology, 3Department of Oncology, the Affiliated Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People&rsquo;s Republic of China; 2Department of Hematology, the Affiliated People&rsquo;s Hospital, Jiangsu University, Zhenjiang, People&rsquo;s Republic of China; 4Institution of Physiology, 6State Key Lab of Bioelectronics (Chien-shiung Wu Laboratory), Southeast University, Nanjing, People&rsquo;s Republic of China; 5Department of Physics, University of Saarland, Saarbruechen, GermanyAbstract: Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy. We evaluated the effect of daunorubicin (DNR)-loaded magnetic nanoparticles of Fe3O4 (MNPs-Fe3O4) on K562-n/VCR cells in vivo. K562-n and its MDR counterpart K562-n/VCR cell were inoculated into nude mice subcutaneously. The mice were randomly divided into four groups: group A received normal saline, group B received DNR, group C received MNPs-Fe3O4, and group D received DNR-loaded MNPs-Fe3O4. For K562-n/VCR tumor, the weight was markedly lower in group D than that in groups A, B, and C. The transcriptions of Mdr-1 and Bcl-2 gene were significantly lower in group D than those in groups A, B, and C. The expression of Bcl-2 was lower in group D than those in groups A, B, and C, but there was no difference in the expression of P-glycoprotein. The transcriptions and expressions of Bax and caspase-3 in group D were increased significantly when compared with groups A, B, and C. In conclusion, DNR-loaded MNPs-Fe3O4 can overcome MDR in vivo.Keywords: multidrug-resistance reversal, leukemia, magnetic nanoparticles of Fe3O4, in vivo Bao-an ChenBin-bin LaiJian Chenget alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2009, Iss default, Pp 201-208 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Bao-an Chen Bin-bin Lai Jian Cheng et al Daunorubicin-loaded magnetic nanoparticles of Fe3O4 overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo
description	Bao-an Chen1, Bin-bin Lai1, Jian Cheng1, Guo-hua Xia1, Feng Gao1, Wen-lin Xu2, Jia-hua Ding1, Chong Gao1, Xin-chen Sun3, Cui-rong Xu1, Wen-ji Chen1, Ning-na Chen1, Li-jie Liu4, Xiao-mao Li5, Xue-mei Wang61Department of Hematology, 3Department of Oncology, the Affiliated Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People&rsquo;s Republic of China; 2Department of Hematology, the Affiliated People&rsquo;s Hospital, Jiangsu University, Zhenjiang, People&rsquo;s Republic of China; 4Institution of Physiology, 6State Key Lab of Bioelectronics (Chien-shiung Wu Laboratory), Southeast University, Nanjing, People&rsquo;s Republic of China; 5Department of Physics, University of Saarland, Saarbruechen, GermanyAbstract: Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy. We evaluated the effect of daunorubicin (DNR)-loaded magnetic nanoparticles of Fe3O4 (MNPs-Fe3O4) on K562-n/VCR cells in vivo. K562-n and its MDR counterpart K562-n/VCR cell were inoculated into nude mice subcutaneously. The mice were randomly divided into four groups: group A received normal saline, group B received DNR, group C received MNPs-Fe3O4, and group D received DNR-loaded MNPs-Fe3O4. For K562-n/VCR tumor, the weight was markedly lower in group D than that in groups A, B, and C. The transcriptions of Mdr-1 and Bcl-2 gene were significantly lower in group D than those in groups A, B, and C. The expression of Bcl-2 was lower in group D than those in groups A, B, and C, but there was no difference in the expression of P-glycoprotein. The transcriptions and expressions of Bax and caspase-3 in group D were increased significantly when compared with groups A, B, and C. In conclusion, DNR-loaded MNPs-Fe3O4 can overcome MDR in vivo.Keywords: multidrug-resistance reversal, leukemia, magnetic nanoparticles of Fe3O4, in vivo
format	article
author	Bao-an Chen Bin-bin Lai Jian Cheng et al
author_facet	Bao-an Chen Bin-bin Lai Jian Cheng et al
author_sort	Bao-an Chen
title	Daunorubicin-loaded magnetic nanoparticles of Fe3O4 overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo
title_short	Daunorubicin-loaded magnetic nanoparticles of Fe3O4 overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo
title_full	Daunorubicin-loaded magnetic nanoparticles of Fe3O4 overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo
title_fullStr	Daunorubicin-loaded magnetic nanoparticles of Fe3O4 overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo
title_full_unstemmed	Daunorubicin-loaded magnetic nanoparticles of Fe3O4 overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo
title_sort	daunorubicin-loaded magnetic nanoparticles of fe3o4 overcome multidrug resistance and induce apoptosis of k562-n/vcr cells in vivo
publisher	Dove Medical Press
publishDate	2009
url	https://doaj.org/article/5fca087d48fe4a589756b55ee4308ece
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Daunorubicin-loaded magnetic nanoparticles of Fe3O4 overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo

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