Antigenicity of Bovine Pericardium Determined by a Novel Immunoproteomic Approach

Abstract Despite bovine pericardium (BP) being the primary biomaterial used in heart valve bioprostheses, recipient graft-specific immune responses remain a significant cause of graft failure. Consequently, tissue antigenicity remains the principal barrier for expanding use of such biomaterials in c...

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Autores principales:	Katherine V. Gates, Ailsa J. Dalgliesh, Leigh G. Griffiths
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5fcaf593be4f4fc2abdf5ff8840be416
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spelling	oai:doaj.org-article:5fcaf593be4f4fc2abdf5ff8840be4162021-12-02T16:06:47ZAntigenicity of Bovine Pericardium Determined by a Novel Immunoproteomic Approach10.1038/s41598-017-02719-82045-2322https://doaj.org/article/5fcaf593be4f4fc2abdf5ff8840be4162017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02719-8https://doaj.org/toc/2045-2322Abstract Despite bovine pericardium (BP) being the primary biomaterial used in heart valve bioprostheses, recipient graft-specific immune responses remain a significant cause of graft failure. Consequently, tissue antigenicity remains the principal barrier for expanding use of such biomaterials in clinical practice. We hypothesize that our understanding of BP antigenicity can be improved by application of a combined affinity chromatography shotgun immunoproteomic approach to identify antigens that have previously been overlooked. Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) analysis of affinity chromatography purified antigens resulted in identification of 133 antigens. Most importantly, antigens were identified from all subcellular locations, including 18 integral membrane protein antigens. Critically, isoforms of several protein families were found to be antigenic suggesting the possibility that shared epitope domains may exist. Furthermore, proteins associated with immune, coagulation, and inflammatory pathways were over-represented, suggesting that these biological processes play a key role in antigenicity. This study brings to light important determinants of antigenicity in a clinically relevant xenogeneic biomaterial (i.e. BP) and further validates a rapid, high-throughput method for immunoproteomic antigen identification.Katherine V. GatesAilsa J. DalglieshLeigh G. GriffithsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katherine V. Gates Ailsa J. Dalgliesh Leigh G. Griffiths Antigenicity of Bovine Pericardium Determined by a Novel Immunoproteomic Approach
description	Abstract Despite bovine pericardium (BP) being the primary biomaterial used in heart valve bioprostheses, recipient graft-specific immune responses remain a significant cause of graft failure. Consequently, tissue antigenicity remains the principal barrier for expanding use of such biomaterials in clinical practice. We hypothesize that our understanding of BP antigenicity can be improved by application of a combined affinity chromatography shotgun immunoproteomic approach to identify antigens that have previously been overlooked. Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) analysis of affinity chromatography purified antigens resulted in identification of 133 antigens. Most importantly, antigens were identified from all subcellular locations, including 18 integral membrane protein antigens. Critically, isoforms of several protein families were found to be antigenic suggesting the possibility that shared epitope domains may exist. Furthermore, proteins associated with immune, coagulation, and inflammatory pathways were over-represented, suggesting that these biological processes play a key role in antigenicity. This study brings to light important determinants of antigenicity in a clinically relevant xenogeneic biomaterial (i.e. BP) and further validates a rapid, high-throughput method for immunoproteomic antigen identification.
format	article
author	Katherine V. Gates Ailsa J. Dalgliesh Leigh G. Griffiths
author_facet	Katherine V. Gates Ailsa J. Dalgliesh Leigh G. Griffiths
author_sort	Katherine V. Gates
title	Antigenicity of Bovine Pericardium Determined by a Novel Immunoproteomic Approach
title_short	Antigenicity of Bovine Pericardium Determined by a Novel Immunoproteomic Approach
title_full	Antigenicity of Bovine Pericardium Determined by a Novel Immunoproteomic Approach
title_fullStr	Antigenicity of Bovine Pericardium Determined by a Novel Immunoproteomic Approach
title_full_unstemmed	Antigenicity of Bovine Pericardium Determined by a Novel Immunoproteomic Approach
title_sort	antigenicity of bovine pericardium determined by a novel immunoproteomic approach
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5fcaf593be4f4fc2abdf5ff8840be416
work_keys_str_mv	AT katherinevgates antigenicityofbovinepericardiumdeterminedbyanovelimmunoproteomicapproach AT ailsajdalgliesh antigenicityofbovinepericardiumdeterminedbyanovelimmunoproteomicapproach AT leighggriffiths antigenicityofbovinepericardiumdeterminedbyanovelimmunoproteomicapproach
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Antigenicity of Bovine Pericardium Determined by a Novel Immunoproteomic Approach

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