HAGLR promotes neuron differentiation through the miR-130a-3p-MeCP2 axis

Parkinson’s disease (PD) is a prevalent neurodegenerative disease. Currently, the molecular mechanisms underlying the progressions of PD are not fully understood. The human neuroblastoma cell line SH-SY5Y has been widely used as an in vitro model for PD. This study aims to investigate the molecular...

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Autores principales:	Wei Bo, Xiao Gui-rong, Wu Cheng-long, Xu Yi-qin
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	pd neuron differentiation lncrna-haglr mecp2 mir-130a-3p Medicine R
Acceso en línea:	https://doaj.org/article/5fdbc610174d4db4a4fce321a9b72904
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spelling	oai:doaj.org-article:5fdbc610174d4db4a4fce321a9b729042021-12-05T14:10:54ZHAGLR promotes neuron differentiation through the miR-130a-3p-MeCP2 axis2391-546310.1515/med-2021-0301https://doaj.org/article/5fdbc610174d4db4a4fce321a9b729042021-08-01T00:00:00Zhttps://doi.org/10.1515/med-2021-0301https://doaj.org/toc/2391-5463Parkinson’s disease (PD) is a prevalent neurodegenerative disease. Currently, the molecular mechanisms underlying the progressions of PD are not fully understood. The human neuroblastoma cell line SH-SY5Y has been widely used as an in vitro model for PD. This study aims to investigate the molecular mechanisms of the non-coding RNA-mediated SH-SY5Y differentiation induced by retinoic acid (RA). By microArray analysis, lncRNA HAGLR was observed to be significantly upregulated during the RA-induced SH-SY5Y differentiation. Silencing HAGLR blocked the RA-induced SH-SY5Y differentiation. Moreover, bioinformatical analysis illustrated that miR-130a-3p contains binding sites for HAGLR. The RNA-pull down assay and luciferase assay demonstrated that HAGLR functioned as a ceRNA of miR-130a-3p in SH-SY5Y cells. Overexpression of miR-130a-3p effectively inhibited SH-SY5Y differentiation. We identified MeCP2, a vital molecule in neuronal diseases, to be a direct target of miR-130a-3p in SH-SY5Y cells by western blot and luciferase assays. The rescue experiments verified that recovery of miR-130a-3p in HAGLR-overexpressing SH-SY5Y cells could successfully overcome the RA-induced SH-SY5Y differentiation by targeting MeCP2. In summary, this study reveals a potential molecular mechanism for the lncRNA-HAGLR-promoted in vitro neuron differentiation by targeting the miR-130a-3p-MeCP2 axis, contributing to the understanding of the pathogenesis and progression of PD.Wei BoXiao Gui-rongWu Cheng-longXu Yi-qinDe Gruyterarticlepdneuron differentiationlncrna-haglrmecp2mir-130a-3pMedicineRENOpen Medicine, Vol 16, Iss 1, Pp 1121-1131 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pd neuron differentiation lncrna-haglr mecp2 mir-130a-3p Medicine R
spellingShingle	pd neuron differentiation lncrna-haglr mecp2 mir-130a-3p Medicine R Wei Bo Xiao Gui-rong Wu Cheng-long Xu Yi-qin HAGLR promotes neuron differentiation through the miR-130a-3p-MeCP2 axis
description	Parkinson’s disease (PD) is a prevalent neurodegenerative disease. Currently, the molecular mechanisms underlying the progressions of PD are not fully understood. The human neuroblastoma cell line SH-SY5Y has been widely used as an in vitro model for PD. This study aims to investigate the molecular mechanisms of the non-coding RNA-mediated SH-SY5Y differentiation induced by retinoic acid (RA). By microArray analysis, lncRNA HAGLR was observed to be significantly upregulated during the RA-induced SH-SY5Y differentiation. Silencing HAGLR blocked the RA-induced SH-SY5Y differentiation. Moreover, bioinformatical analysis illustrated that miR-130a-3p contains binding sites for HAGLR. The RNA-pull down assay and luciferase assay demonstrated that HAGLR functioned as a ceRNA of miR-130a-3p in SH-SY5Y cells. Overexpression of miR-130a-3p effectively inhibited SH-SY5Y differentiation. We identified MeCP2, a vital molecule in neuronal diseases, to be a direct target of miR-130a-3p in SH-SY5Y cells by western blot and luciferase assays. The rescue experiments verified that recovery of miR-130a-3p in HAGLR-overexpressing SH-SY5Y cells could successfully overcome the RA-induced SH-SY5Y differentiation by targeting MeCP2. In summary, this study reveals a potential molecular mechanism for the lncRNA-HAGLR-promoted in vitro neuron differentiation by targeting the miR-130a-3p-MeCP2 axis, contributing to the understanding of the pathogenesis and progression of PD.
format	article
author	Wei Bo Xiao Gui-rong Wu Cheng-long Xu Yi-qin
author_facet	Wei Bo Xiao Gui-rong Wu Cheng-long Xu Yi-qin
author_sort	Wei Bo
title	HAGLR promotes neuron differentiation through the miR-130a-3p-MeCP2 axis
title_short	HAGLR promotes neuron differentiation through the miR-130a-3p-MeCP2 axis
title_full	HAGLR promotes neuron differentiation through the miR-130a-3p-MeCP2 axis
title_fullStr	HAGLR promotes neuron differentiation through the miR-130a-3p-MeCP2 axis
title_full_unstemmed	HAGLR promotes neuron differentiation through the miR-130a-3p-MeCP2 axis
title_sort	haglr promotes neuron differentiation through the mir-130a-3p-mecp2 axis
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/5fdbc610174d4db4a4fce321a9b72904
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HAGLR promotes neuron differentiation through the miR-130a-3p-MeCP2 axis

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