Periodontitis associates with species-specific gene expression of the oral microbiota

Abstract The purpose of the present investigation was to characterize species-specific bacterial activity of the oral microbiota in periodontitis. We tested the hypotheses that chronic inflammation, i.e., periodontitis, associates with bacterial gene expression of the oral microbiota. Oral microbial...

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Autores principales:	Daniel Belstrøm, Florentin Constancias, Daniela I. Drautz-Moses, Stephan C. Schuster, Mark Veleba, Frédéric Mahé, Michael Givskov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/5fdfdd040ae744b292843a02e2b23a55
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spelling	oai:doaj.org-article:5fdfdd040ae744b292843a02e2b23a552021-12-02T17:27:19ZPeriodontitis associates with species-specific gene expression of the oral microbiota10.1038/s41522-021-00247-y2055-5008https://doaj.org/article/5fdfdd040ae744b292843a02e2b23a552021-09-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00247-yhttps://doaj.org/toc/2055-5008Abstract The purpose of the present investigation was to characterize species-specific bacterial activity of the oral microbiota in periodontitis. We tested the hypotheses that chronic inflammation, i.e., periodontitis, associates with bacterial gene expression of the oral microbiota. Oral microbial samples were collected from three oral sites—subgingival plaque, tongue, and saliva from patients with periodontitis and healthy controls. Paired metagenomics and metatranscriptomics were used to perform concomitant characterization of taxonomic composition and to determine species-specific bacterial activity as expressed by the ratio of specific messenger RNA reads to their corresponding genomic DNA reads. Here, we show the association of periodontitis with bacterial gene expression of the oral microbiota. While oral site was the main determinant of taxonomic composition as well as bacterial gene expression, periodontitis was significantly associated with a reduction of carbohydrate metabolism of the oral microbiota at three oral sites (subgingival plaque, tongue, and saliva). Data from the present study revealed the association of periodontitis with bacterial gene expression of the oral microbiota. Conditions of periodontitis was associated with bacterial activity of local subgingival plaque, but also on tongue and the salivary microbiota. Collectively, data suggest that periodontitis associates with impaired carbohydrate metabolism of the oral microbiota. Future longitudinal and interventional studies are warranted to evaluate the potential pathogenic role of impaired bacterial carbohydrate metabolism not only in periodontitis but also in other diseases with low-grade inflammation, such as type 2 diabetes mellitus.Daniel BelstrømFlorentin ConstanciasDaniela I. Drautz-MosesStephan C. SchusterMark VelebaFrédéric MahéMichael GivskovNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Daniel Belstrøm Florentin Constancias Daniela I. Drautz-Moses Stephan C. Schuster Mark Veleba Frédéric Mahé Michael Givskov Periodontitis associates with species-specific gene expression of the oral microbiota
description	Abstract The purpose of the present investigation was to characterize species-specific bacterial activity of the oral microbiota in periodontitis. We tested the hypotheses that chronic inflammation, i.e., periodontitis, associates with bacterial gene expression of the oral microbiota. Oral microbial samples were collected from three oral sites—subgingival plaque, tongue, and saliva from patients with periodontitis and healthy controls. Paired metagenomics and metatranscriptomics were used to perform concomitant characterization of taxonomic composition and to determine species-specific bacterial activity as expressed by the ratio of specific messenger RNA reads to their corresponding genomic DNA reads. Here, we show the association of periodontitis with bacterial gene expression of the oral microbiota. While oral site was the main determinant of taxonomic composition as well as bacterial gene expression, periodontitis was significantly associated with a reduction of carbohydrate metabolism of the oral microbiota at three oral sites (subgingival plaque, tongue, and saliva). Data from the present study revealed the association of periodontitis with bacterial gene expression of the oral microbiota. Conditions of periodontitis was associated with bacterial activity of local subgingival plaque, but also on tongue and the salivary microbiota. Collectively, data suggest that periodontitis associates with impaired carbohydrate metabolism of the oral microbiota. Future longitudinal and interventional studies are warranted to evaluate the potential pathogenic role of impaired bacterial carbohydrate metabolism not only in periodontitis but also in other diseases with low-grade inflammation, such as type 2 diabetes mellitus.
format	article
author	Daniel Belstrøm Florentin Constancias Daniela I. Drautz-Moses Stephan C. Schuster Mark Veleba Frédéric Mahé Michael Givskov
author_facet	Daniel Belstrøm Florentin Constancias Daniela I. Drautz-Moses Stephan C. Schuster Mark Veleba Frédéric Mahé Michael Givskov
author_sort	Daniel Belstrøm
title	Periodontitis associates with species-specific gene expression of the oral microbiota
title_short	Periodontitis associates with species-specific gene expression of the oral microbiota
title_full	Periodontitis associates with species-specific gene expression of the oral microbiota
title_fullStr	Periodontitis associates with species-specific gene expression of the oral microbiota
title_full_unstemmed	Periodontitis associates with species-specific gene expression of the oral microbiota
title_sort	periodontitis associates with species-specific gene expression of the oral microbiota
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5fdfdd040ae744b292843a02e2b23a55
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Periodontitis associates with species-specific gene expression of the oral microbiota

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