Innovative biodegradable poly(L-lactide)/collagen/hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation

Guoqiang Zhou,1–3 Sudan Liu,1 Yanyan Ma,1 Wenshi Xu,1 Wei Meng,1 Xue Lin,1 Wenying Wang,1,3 Shuxiang Wang,1–3 Jinchao Zhang1–3 1College of Chemistry and Environmental Science, 2Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, 3Key...

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Autores principales:	Zhou GQ, Liu SD, Ma YY, Xu WS, Meng W, Lin X, Wang WY, Wang SX, Zhang JC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	nanofibrous scaffolds hydroxyapatite electrospinning tissue engineering degradation differentiation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5fe3fb046da24ae8baf9369653aec86f
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spelling	oai:doaj.org-article:5fe3fb046da24ae8baf9369653aec86f2021-12-02T03:58:29ZInnovative biodegradable poly(L-lactide)/collagen/hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation1178-2013https://doaj.org/article/5fe3fb046da24ae8baf9369653aec86f2017-10-01T00:00:00Zhttps://www.dovepress.com/innovative-biodegradable-polyl-lactidecollagenhydroxyapatite-composite-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Guoqiang Zhou,1–3 Sudan Liu,1 Yanyan Ma,1 Wenshi Xu,1 Wei Meng,1 Xue Lin,1 Wenying Wang,1,3 Shuxiang Wang,1–3 Jinchao Zhang1–3 1College of Chemistry and Environmental Science, 2Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, 3Key Laboratory of Chemical Biology of Hebei Province, Hebei University, Baoding, Hebei, People’s Republic of China Abstract: The development of an artificial bone graft which can promote the regeneration of fractures or diseased bones is currently the most challenging aspect in bone tissue engineering. To achieve the purpose of promoting bone proliferation and differentiation, the artificial graft needs have a similar structure and composition of extracellular matrix. One-step electrospinning method of biocomposite nanofibers containing hydroxyapatite (HA) nanoparticles and collagen (Coll) were developed for potential application in bone tissue engineering. Nanocomposite scaffolds of poly(L-lactide) (PLLA), PLLA/HA, PLLA/Coll, and PLLA/Coll/HA were fabricated by electrospinning. The morphology, diameter, elements, hydrophilicity, and biodegradability of the composite scaffolds have been investigated. The biocompatibility of different nanocomposite scaffolds was assessed using mouse osteoblasts MC3T3-E1 in vitro, and the proliferation, differentiation, and mineralization of cells on different nanofibrous scaffolds were investigated. The results showed that PLLA/Coll/HA nanofiber scaffolds enhanced cell adhesion, spreading, proliferation, differentiation, mineralization, and gene expression of osteogenic markers compared to other scaffolds. In addition, the nanofibrous scaffolds maintained a stable composition at the beginning of the degradation period and morphology wastage and weight loss were observed when incubated for up to 80 days in physiological simulated conditions. The PLLA/Coll/HA composite nanofibrous scaffolds could be a potential material for guided bone regeneration. Keywords: nanofibrous scaffolds, hydroxyapatite, electrospinning, tissue engineering, degradation, differentiationZhou GQLiu SDMa YYXu WSMeng WLin XWang WYWang SXZhang JCDove Medical Pressarticlenanofibrous scaffoldshydroxyapatiteelectrospinningtissue engineeringdegradationdifferentiationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7577-7588 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanofibrous scaffolds hydroxyapatite electrospinning tissue engineering degradation differentiation Medicine (General) R5-920
spellingShingle	nanofibrous scaffolds hydroxyapatite electrospinning tissue engineering degradation differentiation Medicine (General) R5-920 Zhou GQ Liu SD Ma YY Xu WS Meng W Lin X Wang WY Wang SX Zhang JC Innovative biodegradable poly(L-lactide)/collagen/hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation
description	Guoqiang Zhou,1–3 Sudan Liu,1 Yanyan Ma,1 Wenshi Xu,1 Wei Meng,1 Xue Lin,1 Wenying Wang,1,3 Shuxiang Wang,1–3 Jinchao Zhang1–3 1College of Chemistry and Environmental Science, 2Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, 3Key Laboratory of Chemical Biology of Hebei Province, Hebei University, Baoding, Hebei, People’s Republic of China Abstract: The development of an artificial bone graft which can promote the regeneration of fractures or diseased bones is currently the most challenging aspect in bone tissue engineering. To achieve the purpose of promoting bone proliferation and differentiation, the artificial graft needs have a similar structure and composition of extracellular matrix. One-step electrospinning method of biocomposite nanofibers containing hydroxyapatite (HA) nanoparticles and collagen (Coll) were developed for potential application in bone tissue engineering. Nanocomposite scaffolds of poly(L-lactide) (PLLA), PLLA/HA, PLLA/Coll, and PLLA/Coll/HA were fabricated by electrospinning. The morphology, diameter, elements, hydrophilicity, and biodegradability of the composite scaffolds have been investigated. The biocompatibility of different nanocomposite scaffolds was assessed using mouse osteoblasts MC3T3-E1 in vitro, and the proliferation, differentiation, and mineralization of cells on different nanofibrous scaffolds were investigated. The results showed that PLLA/Coll/HA nanofiber scaffolds enhanced cell adhesion, spreading, proliferation, differentiation, mineralization, and gene expression of osteogenic markers compared to other scaffolds. In addition, the nanofibrous scaffolds maintained a stable composition at the beginning of the degradation period and morphology wastage and weight loss were observed when incubated for up to 80 days in physiological simulated conditions. The PLLA/Coll/HA composite nanofibrous scaffolds could be a potential material for guided bone regeneration. Keywords: nanofibrous scaffolds, hydroxyapatite, electrospinning, tissue engineering, degradation, differentiation
format	article
author	Zhou GQ Liu SD Ma YY Xu WS Meng W Lin X Wang WY Wang SX Zhang JC
author_facet	Zhou GQ Liu SD Ma YY Xu WS Meng W Lin X Wang WY Wang SX Zhang JC
author_sort	Zhou GQ
title	Innovative biodegradable poly(L-lactide)/collagen/hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation
title_short	Innovative biodegradable poly(L-lactide)/collagen/hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation
title_full	Innovative biodegradable poly(L-lactide)/collagen/hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation
title_fullStr	Innovative biodegradable poly(L-lactide)/collagen/hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation
title_full_unstemmed	Innovative biodegradable poly(L-lactide)/collagen/hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation
title_sort	innovative biodegradable poly(l-lactide)/collagen/hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/5fe3fb046da24ae8baf9369653aec86f
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_version_	1718401490745294848

Innovative biodegradable poly(L-lactide)/collagen/hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation

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