The phosphate transporter PiT1 (Slc20a1) revealed as a new essential gene for mouse liver development.

<h4>Background</h4>PiT1 (or SLC20a1) encodes a widely expressed plasma membrane protein functioning as a high-affinity Na(+)-phosphate (Pi) cotransporter. As such, PiT1 is often considered as a ubiquitous supplier of Pi for cellular needs regardless of the lack of experimental data. Alth...

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Autores principales:	Laurent Beck, Christine Leroy, Sarah Beck-Cormier, Anne Forand, Christine Salaün, Nadine Paris, Adeline Bernier, Pablo Ureña-Torres, Dominique Prié, Mario Ollero, Laure Coulombel, Gérard Friedlander
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5fe5fb6f9a31451fbc63db8595e4094d
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spelling	oai:doaj.org-article:5fe5fb6f9a31451fbc63db8595e4094d2021-11-25T06:25:57ZThe phosphate transporter PiT1 (Slc20a1) revealed as a new essential gene for mouse liver development.1932-620310.1371/journal.pone.0009148https://doaj.org/article/5fe5fb6f9a31451fbc63db8595e4094d2010-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20161774/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>PiT1 (or SLC20a1) encodes a widely expressed plasma membrane protein functioning as a high-affinity Na(+)-phosphate (Pi) cotransporter. As such, PiT1 is often considered as a ubiquitous supplier of Pi for cellular needs regardless of the lack of experimental data. Although the importance of PiT1 in mineralizing processes have been demonstrated in vitro in osteoblasts, chondrocytes and vascular smooth muscle cells, in vivo evidence is missing.<h4>Methodology/principal findings</h4>To determine the in vivo function of PiT1, we generated an allelic series of PiT1 mutations in mice by combination of wild-type, hypomorphic and null PiT1 alleles expressing from 100% to 0% of PiT1. In this report we show that complete deletion of PiT1 results in embryonic lethality at E12.5. PiT1-deficient embryos display severely hypoplastic fetal livers and subsequent reduced hematopoiesis resulting in embryonic death from anemia. We show that the anemia is not due to placental, yolk sac or vascular defects and that hematopoietic progenitors have no cell-autonomous defects in proliferation and differentiation. In contrast, mutant fetal livers display decreased proliferation and massive apoptosis. Animals carrying two copies of hypomorphic PiT1 alleles (resulting in 15% PiT1 expression comparing to wild-type animals) survive at birth but are growth-retarded and anemic. The combination of both hypomorphic and null alleles in heterozygous compounds results in late embryonic lethality (E14.5-E16.5) with phenotypic features intermediate between null and hypomorphic mice. In the three mouse lines generated we could not evidence defects in early skeleton formation.<h4>Conclusion/significance</h4>This work is the first to illustrate a specific in vivo role for PiT1 by uncovering it as being a critical gene for normal developmental liver growth.Laurent BeckChristine LeroySarah Beck-CormierAnne ForandChristine SalaünNadine ParisAdeline BernierPablo Ureña-TorresDominique PriéMario OlleroLaure CoulombelGérard FriedlanderPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 2, p e9148 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laurent Beck Christine Leroy Sarah Beck-Cormier Anne Forand Christine Salaün Nadine Paris Adeline Bernier Pablo Ureña-Torres Dominique Prié Mario Ollero Laure Coulombel Gérard Friedlander The phosphate transporter PiT1 (Slc20a1) revealed as a new essential gene for mouse liver development.
description	<h4>Background</h4>PiT1 (or SLC20a1) encodes a widely expressed plasma membrane protein functioning as a high-affinity Na(+)-phosphate (Pi) cotransporter. As such, PiT1 is often considered as a ubiquitous supplier of Pi for cellular needs regardless of the lack of experimental data. Although the importance of PiT1 in mineralizing processes have been demonstrated in vitro in osteoblasts, chondrocytes and vascular smooth muscle cells, in vivo evidence is missing.<h4>Methodology/principal findings</h4>To determine the in vivo function of PiT1, we generated an allelic series of PiT1 mutations in mice by combination of wild-type, hypomorphic and null PiT1 alleles expressing from 100% to 0% of PiT1. In this report we show that complete deletion of PiT1 results in embryonic lethality at E12.5. PiT1-deficient embryos display severely hypoplastic fetal livers and subsequent reduced hematopoiesis resulting in embryonic death from anemia. We show that the anemia is not due to placental, yolk sac or vascular defects and that hematopoietic progenitors have no cell-autonomous defects in proliferation and differentiation. In contrast, mutant fetal livers display decreased proliferation and massive apoptosis. Animals carrying two copies of hypomorphic PiT1 alleles (resulting in 15% PiT1 expression comparing to wild-type animals) survive at birth but are growth-retarded and anemic. The combination of both hypomorphic and null alleles in heterozygous compounds results in late embryonic lethality (E14.5-E16.5) with phenotypic features intermediate between null and hypomorphic mice. In the three mouse lines generated we could not evidence defects in early skeleton formation.<h4>Conclusion/significance</h4>This work is the first to illustrate a specific in vivo role for PiT1 by uncovering it as being a critical gene for normal developmental liver growth.
format	article
author	Laurent Beck Christine Leroy Sarah Beck-Cormier Anne Forand Christine Salaün Nadine Paris Adeline Bernier Pablo Ureña-Torres Dominique Prié Mario Ollero Laure Coulombel Gérard Friedlander
author_facet	Laurent Beck Christine Leroy Sarah Beck-Cormier Anne Forand Christine Salaün Nadine Paris Adeline Bernier Pablo Ureña-Torres Dominique Prié Mario Ollero Laure Coulombel Gérard Friedlander
author_sort	Laurent Beck
title	The phosphate transporter PiT1 (Slc20a1) revealed as a new essential gene for mouse liver development.
title_short	The phosphate transporter PiT1 (Slc20a1) revealed as a new essential gene for mouse liver development.
title_full	The phosphate transporter PiT1 (Slc20a1) revealed as a new essential gene for mouse liver development.
title_fullStr	The phosphate transporter PiT1 (Slc20a1) revealed as a new essential gene for mouse liver development.
title_full_unstemmed	The phosphate transporter PiT1 (Slc20a1) revealed as a new essential gene for mouse liver development.
title_sort	phosphate transporter pit1 (slc20a1) revealed as a new essential gene for mouse liver development.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/5fe5fb6f9a31451fbc63db8595e4094d
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The phosphate transporter PiT1 (Slc20a1) revealed as a new essential gene for mouse liver development.

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