Transcriptome and proteome data reveal candidate genes for pollinator attraction in sexually deceptive orchids.

<h4>Background</h4>Sexually deceptive orchids of the genus Ophrys mimic the mating signals of their pollinator females to attract males as pollinators. This mode of pollination is highly specific and leads to strong reproductive isolation between species. This study aims to identify cand...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Khalid E M Sedeek, Weihong Qi, Monica A Schauer, Alok K Gupta, Lucy Poveda, Shuqing Xu, Zhong-Jian Liu, Ueli Grossniklaus, Florian P Schiestl, Philipp M Schlüter
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5fec755d332d4f4d92df985194279ba1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5fec755d332d4f4d92df985194279ba1
record_format	dspace
spelling	oai:doaj.org-article:5fec755d332d4f4d92df985194279ba12021-11-18T07:43:56ZTranscriptome and proteome data reveal candidate genes for pollinator attraction in sexually deceptive orchids.1932-620310.1371/journal.pone.0064621https://doaj.org/article/5fec755d332d4f4d92df985194279ba12013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23734209/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Sexually deceptive orchids of the genus Ophrys mimic the mating signals of their pollinator females to attract males as pollinators. This mode of pollination is highly specific and leads to strong reproductive isolation between species. This study aims to identify candidate genes responsible for pollinator attraction and reproductive isolation between three closely related species, O. exaltata, O. sphegodes and O. garganica. Floral traits such as odour, colour and morphology are necessary for successful pollinator attraction. In particular, different odour hydrocarbon profiles have been linked to differences in specific pollinator attraction among these species. Therefore, the identification of genes involved in these traits is important for understanding the molecular basis of pollinator attraction by sexually deceptive orchids.<h4>Results</h4>We have created floral reference transcriptomes and proteomes for these three Ophrys species using a combination of next-generation sequencing (454 and Solexa), Sanger sequencing, and shotgun proteomics (tandem mass spectrometry). In total, 121 917 unique transcripts and 3531 proteins were identified. This represents the first orchid proteome and transcriptome from the orchid subfamily Orchidoideae. Proteome data revealed proteins corresponding to 2644 transcripts and 887 proteins not observed in the transcriptome. Candidate genes for hydrocarbon and anthocyanin biosynthesis were represented by 156 and 61 unique transcripts in 20 and 7 genes classes, respectively. Moreover, transcription factors putatively involved in the regulation of flower odour, colour and morphology were annotated, including Myb, MADS and TCP factors.<h4>Conclusion</h4>Our comprehensive data set generated by combining transcriptome and proteome technologies allowed identification of candidate genes for pollinator attraction and reproductive isolation among sexually deceptive orchids. This includes genes for hydrocarbon and anthocyanin biosynthesis and regulation, and the development of floral morphology. These data will serve as an invaluable resource for research in orchid floral biology, enabling studies into the molecular mechanisms of pollinator attraction and speciation.Khalid E M SedeekWeihong QiMonica A SchauerAlok K GuptaLucy PovedaShuqing XuZhong-Jian LiuUeli GrossniklausFlorian P SchiestlPhilipp M SchlüterPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64621 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Khalid E M Sedeek Weihong Qi Monica A Schauer Alok K Gupta Lucy Poveda Shuqing Xu Zhong-Jian Liu Ueli Grossniklaus Florian P Schiestl Philipp M Schlüter Transcriptome and proteome data reveal candidate genes for pollinator attraction in sexually deceptive orchids.
description	<h4>Background</h4>Sexually deceptive orchids of the genus Ophrys mimic the mating signals of their pollinator females to attract males as pollinators. This mode of pollination is highly specific and leads to strong reproductive isolation between species. This study aims to identify candidate genes responsible for pollinator attraction and reproductive isolation between three closely related species, O. exaltata, O. sphegodes and O. garganica. Floral traits such as odour, colour and morphology are necessary for successful pollinator attraction. In particular, different odour hydrocarbon profiles have been linked to differences in specific pollinator attraction among these species. Therefore, the identification of genes involved in these traits is important for understanding the molecular basis of pollinator attraction by sexually deceptive orchids.<h4>Results</h4>We have created floral reference transcriptomes and proteomes for these three Ophrys species using a combination of next-generation sequencing (454 and Solexa), Sanger sequencing, and shotgun proteomics (tandem mass spectrometry). In total, 121 917 unique transcripts and 3531 proteins were identified. This represents the first orchid proteome and transcriptome from the orchid subfamily Orchidoideae. Proteome data revealed proteins corresponding to 2644 transcripts and 887 proteins not observed in the transcriptome. Candidate genes for hydrocarbon and anthocyanin biosynthesis were represented by 156 and 61 unique transcripts in 20 and 7 genes classes, respectively. Moreover, transcription factors putatively involved in the regulation of flower odour, colour and morphology were annotated, including Myb, MADS and TCP factors.<h4>Conclusion</h4>Our comprehensive data set generated by combining transcriptome and proteome technologies allowed identification of candidate genes for pollinator attraction and reproductive isolation among sexually deceptive orchids. This includes genes for hydrocarbon and anthocyanin biosynthesis and regulation, and the development of floral morphology. These data will serve as an invaluable resource for research in orchid floral biology, enabling studies into the molecular mechanisms of pollinator attraction and speciation.
format	article
author	Khalid E M Sedeek Weihong Qi Monica A Schauer Alok K Gupta Lucy Poveda Shuqing Xu Zhong-Jian Liu Ueli Grossniklaus Florian P Schiestl Philipp M Schlüter
author_facet	Khalid E M Sedeek Weihong Qi Monica A Schauer Alok K Gupta Lucy Poveda Shuqing Xu Zhong-Jian Liu Ueli Grossniklaus Florian P Schiestl Philipp M Schlüter
author_sort	Khalid E M Sedeek
title	Transcriptome and proteome data reveal candidate genes for pollinator attraction in sexually deceptive orchids.
title_short	Transcriptome and proteome data reveal candidate genes for pollinator attraction in sexually deceptive orchids.
title_full	Transcriptome and proteome data reveal candidate genes for pollinator attraction in sexually deceptive orchids.
title_fullStr	Transcriptome and proteome data reveal candidate genes for pollinator attraction in sexually deceptive orchids.
title_full_unstemmed	Transcriptome and proteome data reveal candidate genes for pollinator attraction in sexually deceptive orchids.
title_sort	transcriptome and proteome data reveal candidate genes for pollinator attraction in sexually deceptive orchids.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5fec755d332d4f4d92df985194279ba1
work_keys_str_mv	AT khalidemsedeek transcriptomeandproteomedatarevealcandidategenesforpollinatorattractioninsexuallydeceptiveorchids AT weihongqi transcriptomeandproteomedatarevealcandidategenesforpollinatorattractioninsexuallydeceptiveorchids AT monicaaschauer transcriptomeandproteomedatarevealcandidategenesforpollinatorattractioninsexuallydeceptiveorchids AT alokkgupta transcriptomeandproteomedatarevealcandidategenesforpollinatorattractioninsexuallydeceptiveorchids AT lucypoveda transcriptomeandproteomedatarevealcandidategenesforpollinatorattractioninsexuallydeceptiveorchids AT shuqingxu transcriptomeandproteomedatarevealcandidategenesforpollinatorattractioninsexuallydeceptiveorchids AT zhongjianliu transcriptomeandproteomedatarevealcandidategenesforpollinatorattractioninsexuallydeceptiveorchids AT ueligrossniklaus transcriptomeandproteomedatarevealcandidategenesforpollinatorattractioninsexuallydeceptiveorchids AT florianpschiestl transcriptomeandproteomedatarevealcandidategenesforpollinatorattractioninsexuallydeceptiveorchids AT philippmschluter transcriptomeandproteomedatarevealcandidategenesforpollinatorattractioninsexuallydeceptiveorchids
_version_	1718423039108972544

Transcriptome and proteome data reveal candidate genes for pollinator attraction in sexually deceptive orchids.

Ejemplares similares