Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method

The objectives of this research was to analyze the polymorphisms of SNP g.10428C>T in the SCD gene using PCR-RFLP and its association with meat charactheristics in Bali cattle. The number of 52 Bali cattle from BPTU-HTP Denpasar were used in this study and which were compared to Belgianblue (n=30...

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Autores principales:	S. N. Karimah, R. R. Noor, J. Jakaria
Formato:	article
Lenguaje:	EN
Publicado:	Diponegoro University, Faculty of Animal and Agricultural Sciences 2021
Materias:	bali cattle meat quality pcr-rflp scd gene snp Animal culture SF1-1100 Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/5feec5a18d75412a80cd2326014c5292
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spelling	oai:doaj.org-article:5feec5a18d75412a80cd2326014c52922021-12-02T20:46:58ZIdentification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method2087-82732460-627810.14710/jitaa.46.4.295-303https://doaj.org/article/5feec5a18d75412a80cd2326014c52922021-12-01T00:00:00Zhttps://ejournal.undip.ac.id/index.php/jitaa/article/view/37158https://doaj.org/toc/2087-8273https://doaj.org/toc/2460-6278The objectives of this research was to analyze the polymorphisms of SNP g.10428C>T in the SCD gene using PCR-RFLP and its association with meat charactheristics in Bali cattle. The number of 52 Bali cattle from BPTU-HTP Denpasar were used in this study and which were compared to Belgianblue (n=30), Limousine (n=14), Peranakan Ongole (PO) (n=30) and Wagyu (n=7). The polymorphism of the SCD gene was characterized by the use of the PCR-RFLP technique with HinfI enzyme restriction. The characteristics of the meat quality in Bali cattle were estimated using ultrasound imaging method. Genotyphic and allelic frequencies, heterozygosity values, and the Hardy-Weinberg equilibrium have been calculated using PopGen 1.32. The association between genotypes of SNP g.10428C>T SCD gene and meat quality characteristics was calculated using t-test. The results showed that homozygous genotypes (CC and TT) and heterozygous genotypes (CT) of the SCD\|HinfI gene with C (0.26) and T (0.74) allele frequencies were and in Bali cattle. The CC genotype was found in Belgian-blue, Limousine, PO, and Wagyu cattle with the allele C frequency of 1.00. The observed heterozygosity (Ho=0.44) higher than expected heterozygosity (He=0.39) showed high diversity in Bali cattle. Genotypes of SNP g.10428C>T SCD gene were associated with backfat thickness, marbling score, and percentage of intramuscular fat. Thus the SNPs g.10428C>T have potential as marker-assisted selection candidates for Bali cattle.S. N. KarimahR. R. NoorJ. JakariaDiponegoro University, Faculty of Animal and Agricultural Sciencesarticlebali cattlemeat qualitypcr-rflpscd genesnpAnimal cultureSF1-1100Veterinary medicineSF600-1100ENJournal of the Indonesian Tropical Animal Agriculture, Vol 46, Iss 4, Pp 295-303 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bali cattle meat quality pcr-rflp scd gene snp Animal culture SF1-1100 Veterinary medicine SF600-1100
spellingShingle	bali cattle meat quality pcr-rflp scd gene snp Animal culture SF1-1100 Veterinary medicine SF600-1100 S. N. Karimah R. R. Noor J. Jakaria Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method
description	The objectives of this research was to analyze the polymorphisms of SNP g.10428C>T in the SCD gene using PCR-RFLP and its association with meat charactheristics in Bali cattle. The number of 52 Bali cattle from BPTU-HTP Denpasar were used in this study and which were compared to Belgianblue (n=30), Limousine (n=14), Peranakan Ongole (PO) (n=30) and Wagyu (n=7). The polymorphism of the SCD gene was characterized by the use of the PCR-RFLP technique with HinfI enzyme restriction. The characteristics of the meat quality in Bali cattle were estimated using ultrasound imaging method. Genotyphic and allelic frequencies, heterozygosity values, and the Hardy-Weinberg equilibrium have been calculated using PopGen 1.32. The association between genotypes of SNP g.10428C>T SCD gene and meat quality characteristics was calculated using t-test. The results showed that homozygous genotypes (CC and TT) and heterozygous genotypes (CT) of the SCD\|HinfI gene with C (0.26) and T (0.74) allele frequencies were and in Bali cattle. The CC genotype was found in Belgian-blue, Limousine, PO, and Wagyu cattle with the allele C frequency of 1.00. The observed heterozygosity (Ho=0.44) higher than expected heterozygosity (He=0.39) showed high diversity in Bali cattle. Genotypes of SNP g.10428C>T SCD gene were associated with backfat thickness, marbling score, and percentage of intramuscular fat. Thus the SNPs g.10428C>T have potential as marker-assisted selection candidates for Bali cattle.
format	article
author	S. N. Karimah R. R. Noor J. Jakaria
author_facet	S. N. Karimah R. R. Noor J. Jakaria
author_sort	S. N. Karimah
title	Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method
title_short	Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method
title_full	Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method
title_fullStr	Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method
title_full_unstemmed	Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method
title_sort	identification of snp g.10428c>t of stearoyl-coa desaturase gene related to meat quality in bali cattle using pcr-rflp method
publisher	Diponegoro University, Faculty of Animal and Agricultural Sciences
publishDate	2021
url	https://doaj.org/article/5feec5a18d75412a80cd2326014c5292
work_keys_str_mv	AT snkarimah identificationofsnpg10428ctofstearoylcoadesaturasegenerelatedtomeatqualityinbalicattleusingpcrrflpmethod AT rrnoor identificationofsnpg10428ctofstearoylcoadesaturasegenerelatedtomeatqualityinbalicattleusingpcrrflpmethod AT jjakaria identificationofsnpg10428ctofstearoylcoadesaturasegenerelatedtomeatqualityinbalicattleusingpcrrflpmethod
_version_	1718374053067096064

Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method

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