Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action

There is ongoing research on various herbal bioactives and delivery systems which indicates that both lipid nanocarriers and herbal medicines will be fine tunned and integrated for future bio-medical applications. The current study was undertaken to systematically develop NLC-DSG-yam extract for the...

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Autores principales:	Teodora-Alexandra Iordache, Nicoleta Badea, Mirela Mihaila, Simona Crisan, Anca Lucia Pop, Ioana Lacatusu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	diosgenin-nanocarriers wild yam extract short-life ROS cationic stable radicals Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/6013f1d1c58e40a888b2e956b2036fac
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spelling	oai:doaj.org-article:6013f1d1c58e40a888b2e956b2036fac2021-11-25T18:31:53ZChallenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action10.3390/nano111130352079-4991https://doaj.org/article/6013f1d1c58e40a888b2e956b2036fac2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/3035https://doaj.org/toc/2079-4991There is ongoing research on various herbal bioactives and delivery systems which indicates that both lipid nanocarriers and herbal medicines will be fine tunned and integrated for future bio-medical applications. The current study was undertaken to systematically develop NLC-DSG-yam extract for the improved efficacy of herbal Diosgenin (DSG) in the management of anti-inflammatory disorders. NLCs were characterized regarding the mean size of the particles, morphological characteristics, physical stability in time, thermal behaviour, and entrapment efficiency of the herbal bioactive. Encapsulation efficiency and in vitro antioxidant activity measured the differences between the individual and dual co-loaded-NLC, the co-loaded one assuring a prolonged controlled release of DSG and a more emphasized ability of capturing short-life reactive oxygen species (ROS). NLCs safety properties were monitored following the in vitro MTS ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay) and RTCA (Real-Time Cell Analysis) assays. Concentrations less than 50 μg/mL showed no cytotoxic effects during in vitro cytotoxicity assays. Besides, the NLC-DSG-yam extract revealed a great anti-inflammatory effect, as the production of pro-inflammatory cytokines (TNF-alpha, IL-6) was significantly inhibited at 50 μg/mL NLC (e.g., 98.2% ± 1.07 inhibition of TNF-α, while for IL-6 the inhibition percentage was of 62% ± 1.07). Concluding, using appropriate lipid nanocarriers, the most desirable properties of herbal bioactives could be improved.Teodora-Alexandra IordacheNicoleta BadeaMirela MihailaSimona CrisanAnca Lucia PopIoana LacatusuMDPI AGarticlediosgenin-nanocarrierswild yam extractshort-life ROScationic stable radicalsChemistryQD1-999ENNanomaterials, Vol 11, Iss 3035, p 3035 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diosgenin-nanocarriers wild yam extract short-life ROS cationic stable radicals Chemistry QD1-999
spellingShingle	diosgenin-nanocarriers wild yam extract short-life ROS cationic stable radicals Chemistry QD1-999 Teodora-Alexandra Iordache Nicoleta Badea Mirela Mihaila Simona Crisan Anca Lucia Pop Ioana Lacatusu Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action
description	There is ongoing research on various herbal bioactives and delivery systems which indicates that both lipid nanocarriers and herbal medicines will be fine tunned and integrated for future bio-medical applications. The current study was undertaken to systematically develop NLC-DSG-yam extract for the improved efficacy of herbal Diosgenin (DSG) in the management of anti-inflammatory disorders. NLCs were characterized regarding the mean size of the particles, morphological characteristics, physical stability in time, thermal behaviour, and entrapment efficiency of the herbal bioactive. Encapsulation efficiency and in vitro antioxidant activity measured the differences between the individual and dual co-loaded-NLC, the co-loaded one assuring a prolonged controlled release of DSG and a more emphasized ability of capturing short-life reactive oxygen species (ROS). NLCs safety properties were monitored following the in vitro MTS ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay) and RTCA (Real-Time Cell Analysis) assays. Concentrations less than 50 μg/mL showed no cytotoxic effects during in vitro cytotoxicity assays. Besides, the NLC-DSG-yam extract revealed a great anti-inflammatory effect, as the production of pro-inflammatory cytokines (TNF-alpha, IL-6) was significantly inhibited at 50 μg/mL NLC (e.g., 98.2% ± 1.07 inhibition of TNF-α, while for IL-6 the inhibition percentage was of 62% ± 1.07). Concluding, using appropriate lipid nanocarriers, the most desirable properties of herbal bioactives could be improved.
format	article
author	Teodora-Alexandra Iordache Nicoleta Badea Mirela Mihaila Simona Crisan Anca Lucia Pop Ioana Lacatusu
author_facet	Teodora-Alexandra Iordache Nicoleta Badea Mirela Mihaila Simona Crisan Anca Lucia Pop Ioana Lacatusu
author_sort	Teodora-Alexandra Iordache
title	Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action
title_short	Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action
title_full	Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action
title_fullStr	Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action
title_full_unstemmed	Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action
title_sort	challenges in coopted hydrophilic and lipophilic herbal bioactives in the same nanostructured carriers for effective bioavailability and anti-inflammatory action
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6013f1d1c58e40a888b2e956b2036fac
work_keys_str_mv	AT teodoraalexandraiordache challengesincooptedhydrophilicandlipophilicherbalbioactivesinthesamenanostructuredcarriersforeffectivebioavailabilityandantiinflammatoryaction AT nicoletabadea challengesincooptedhydrophilicandlipophilicherbalbioactivesinthesamenanostructuredcarriersforeffectivebioavailabilityandantiinflammatoryaction AT mirelamihaila challengesincooptedhydrophilicandlipophilicherbalbioactivesinthesamenanostructuredcarriersforeffectivebioavailabilityandantiinflammatoryaction AT simonacrisan challengesincooptedhydrophilicandlipophilicherbalbioactivesinthesamenanostructuredcarriersforeffectivebioavailabilityandantiinflammatoryaction AT ancaluciapop challengesincooptedhydrophilicandlipophilicherbalbioactivesinthesamenanostructuredcarriersforeffectivebioavailabilityandantiinflammatoryaction AT ioanalacatusu challengesincooptedhydrophilicandlipophilicherbalbioactivesinthesamenanostructuredcarriersforeffectivebioavailabilityandantiinflammatoryaction
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Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action

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