The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a <i>Streptococcus mutans</i> Cultivated Environment

The aim of this study was to evaluate the remineralization and antibacterial effect of silver-containing mesoporous bioactive glass (MBG-Ag) sealing combined with Er:YAG laser irradiation on human demineralized dentin specimens in a <i>Streptococcus mutans</i> cultivated environment. A t...

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Autores principales:	Jung-Chang Kung, Wei-Hsun Wang, Yu-Ching Chiang, Yuan-Ting Yang-Wang, Yueh-Ching Wang, Wen-Cheng Chen, Chi-Jen Shih
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antibacterial remineralization Er-YAG laser dentinal tubules <i>Streptococcus mutans</i> dentin hypersensitivity Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/60153987610545a890920232da90d542
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spelling	oai:doaj.org-article:60153987610545a890920232da90d5422021-11-25T18:39:32ZThe Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a <i>Streptococcus mutans</i> Cultivated Environment10.3390/ph141111241424-8247https://doaj.org/article/60153987610545a890920232da90d5422021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1124https://doaj.org/toc/1424-8247The aim of this study was to evaluate the remineralization and antibacterial effect of silver-containing mesoporous bioactive glass (MBG-Ag) sealing combined with Er:YAG laser irradiation on human demineralized dentin specimens in a <i>Streptococcus mutans</i> cultivated environment. A total of 48 human dentin specimens were randomly divided into four groups. The characteristics of MBG-Ag and the occlusion efficiency of the dentinal tubules were analyzed using X-ray diffraction patterns, Fourier-transform infrared spectroscopy, scanning electron microscope images and energy dispersive X-ray spectroscopy. Moreover, the antibacterial activity against <i>Streptococcus mutans</i> was evaluated by colony formation assay. The results showed that the dentin specimens with Er:YAG laser irradiation can form a melted occlusion with a size of 3–4 µm. MBG-Ag promoted the deposition of numerous crystal particles on the dentinal surface, reaching the deepest penetration depth of 70 μm. The results suggested that both MBG-Ag and laser have the ability to enhance the remineralization and precipitation of hydroxyapatite crystals. While the results showed that MBG-Ag sealing combined with the thermomechanical subablation mode of Er:YAG laser irradiation-induced dense crystalline deposition, reaching a penetration depth of more than 300 µm, silver nanoparticles without good absorption of the Er:YAG laser resulted in a heterogeneous radiated surface. Er:YAG laser irradiation with a low energy and pulse rate cannot completely inhibit the growth of <i>S. mutans</i>, but MBG-Ag sealing reached the bactericidal concentration. It was concluded that the simultaneous application of MBG-Ag sealing and Er:YAG laser treatment can prevent the drawbacks of their independent uses, resulting in a superior form of treatment for dentin hypersensitivity.Jung-Chang KungWei-Hsun WangYu-Ching ChiangYuan-Ting Yang-WangYueh-Ching WangWen-Cheng ChenChi-Jen ShihMDPI AGarticleantibacterialremineralizationEr-YAG laserdentinal tubules<i>Streptococcus mutans</i>dentin hypersensitivityMedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1124, p 1124 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibacterial remineralization Er-YAG laser dentinal tubules <i>Streptococcus mutans</i> dentin hypersensitivity Medicine R Pharmacy and materia medica RS1-441
spellingShingle	antibacterial remineralization Er-YAG laser dentinal tubules <i>Streptococcus mutans</i> dentin hypersensitivity Medicine R Pharmacy and materia medica RS1-441 Jung-Chang Kung Wei-Hsun Wang Yu-Ching Chiang Yuan-Ting Yang-Wang Yueh-Ching Wang Wen-Cheng Chen Chi-Jen Shih The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a <i>Streptococcus mutans</i> Cultivated Environment
description	The aim of this study was to evaluate the remineralization and antibacterial effect of silver-containing mesoporous bioactive glass (MBG-Ag) sealing combined with Er:YAG laser irradiation on human demineralized dentin specimens in a <i>Streptococcus mutans</i> cultivated environment. A total of 48 human dentin specimens were randomly divided into four groups. The characteristics of MBG-Ag and the occlusion efficiency of the dentinal tubules were analyzed using X-ray diffraction patterns, Fourier-transform infrared spectroscopy, scanning electron microscope images and energy dispersive X-ray spectroscopy. Moreover, the antibacterial activity against <i>Streptococcus mutans</i> was evaluated by colony formation assay. The results showed that the dentin specimens with Er:YAG laser irradiation can form a melted occlusion with a size of 3–4 µm. MBG-Ag promoted the deposition of numerous crystal particles on the dentinal surface, reaching the deepest penetration depth of 70 μm. The results suggested that both MBG-Ag and laser have the ability to enhance the remineralization and precipitation of hydroxyapatite crystals. While the results showed that MBG-Ag sealing combined with the thermomechanical subablation mode of Er:YAG laser irradiation-induced dense crystalline deposition, reaching a penetration depth of more than 300 µm, silver nanoparticles without good absorption of the Er:YAG laser resulted in a heterogeneous radiated surface. Er:YAG laser irradiation with a low energy and pulse rate cannot completely inhibit the growth of <i>S. mutans</i>, but MBG-Ag sealing reached the bactericidal concentration. It was concluded that the simultaneous application of MBG-Ag sealing and Er:YAG laser treatment can prevent the drawbacks of their independent uses, resulting in a superior form of treatment for dentin hypersensitivity.
format	article
author	Jung-Chang Kung Wei-Hsun Wang Yu-Ching Chiang Yuan-Ting Yang-Wang Yueh-Ching Wang Wen-Cheng Chen Chi-Jen Shih
author_facet	Jung-Chang Kung Wei-Hsun Wang Yu-Ching Chiang Yuan-Ting Yang-Wang Yueh-Ching Wang Wen-Cheng Chen Chi-Jen Shih
author_sort	Jung-Chang Kung
title	The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a <i>Streptococcus mutans</i> Cultivated Environment
title_short	The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a <i>Streptococcus mutans</i> Cultivated Environment
title_full	The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a <i>Streptococcus mutans</i> Cultivated Environment
title_fullStr	The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a <i>Streptococcus mutans</i> Cultivated Environment
title_full_unstemmed	The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a <i>Streptococcus mutans</i> Cultivated Environment
title_sort	antibacterial and remineralization effect of silver-containing mesoporous bioactive glass sealing and er-yag laser on dentinal tubules treated in a <i>streptococcus mutans</i> cultivated environment
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/60153987610545a890920232da90d542
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The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a <i>Streptococcus mutans</i> Cultivated Environment

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