Insights into eyestalk ablation mechanism to induce ovarian maturation in the black tiger shrimp.
Eyestalk ablation is commonly practiced in crustacean to induce ovarian maturation in captivity. The molecular mechanism of the ablation has not been well understood, preventing a search for alternative measures to induce ovarian maturation in aquaculture. This is the first study to employ cDNA micr...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2011
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/6016aafac90e42c9ad537248ed1342cc |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:6016aafac90e42c9ad537248ed1342cc |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:6016aafac90e42c9ad537248ed1342cc2021-11-18T06:46:32ZInsights into eyestalk ablation mechanism to induce ovarian maturation in the black tiger shrimp.1932-620310.1371/journal.pone.0024427https://doaj.org/article/6016aafac90e42c9ad537248ed1342cc2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21915325/?tool=EBIhttps://doaj.org/toc/1932-6203Eyestalk ablation is commonly practiced in crustacean to induce ovarian maturation in captivity. The molecular mechanism of the ablation has not been well understood, preventing a search for alternative measures to induce ovarian maturation in aquaculture. This is the first study to employ cDNA microarray to examine effects of eyestalk ablation at the transcriptomic level and pathway mapping analysis to identify potentially affected biological pathways in the black tiger shrimp (Penaeus monodon). Microarray analysis comparing between gene expression levels of ovaries from eyestalk-intact and eyestalk-ablated brooders revealed 682 differentially expressed transcripts. Based on Hierarchical clustering of gene expression patterns, Gene Ontology annotation, and relevant functions of these differentially expressed genes, several gene groups were further examined by pathway mapping analysis. Reverse-transcriptase quantitative PCR analysis for some representative transcripts confirmed microarray data. Known reproductive genes involved in vitellogenesis were dramatically increased during the ablation. Besides these transcripts expected to be induced by the ablation, transcripts whose functions involved in electron transfer mechanism, immune responses and calcium signal transduction were significantly altered following the ablation. Pathway mapping analysis revealed that the activation of gonadotropin-releasing hormone signaling, calcium signaling, and progesterone-mediated oocyte maturation pathways were putatively crucial to ovarian maturation induced by the ablation. These findings shed light on several possible molecular mechanisms of the eyestalk ablation effect and allow more focused investigation for an ultimate goal of finding alternative methods to replace the undesirable practice of the eyestalk ablation in the future.Umaporn UawisetwathanaRungnapa LeelatanawitAmornpan KlanchuiJuthatip PrommoonSirawut KlinbungaNitsara KaroonuthaisiriPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24427 (2011) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Umaporn Uawisetwathana Rungnapa Leelatanawit Amornpan Klanchui Juthatip Prommoon Sirawut Klinbunga Nitsara Karoonuthaisiri Insights into eyestalk ablation mechanism to induce ovarian maturation in the black tiger shrimp. |
| description |
Eyestalk ablation is commonly practiced in crustacean to induce ovarian maturation in captivity. The molecular mechanism of the ablation has not been well understood, preventing a search for alternative measures to induce ovarian maturation in aquaculture. This is the first study to employ cDNA microarray to examine effects of eyestalk ablation at the transcriptomic level and pathway mapping analysis to identify potentially affected biological pathways in the black tiger shrimp (Penaeus monodon). Microarray analysis comparing between gene expression levels of ovaries from eyestalk-intact and eyestalk-ablated brooders revealed 682 differentially expressed transcripts. Based on Hierarchical clustering of gene expression patterns, Gene Ontology annotation, and relevant functions of these differentially expressed genes, several gene groups were further examined by pathway mapping analysis. Reverse-transcriptase quantitative PCR analysis for some representative transcripts confirmed microarray data. Known reproductive genes involved in vitellogenesis were dramatically increased during the ablation. Besides these transcripts expected to be induced by the ablation, transcripts whose functions involved in electron transfer mechanism, immune responses and calcium signal transduction were significantly altered following the ablation. Pathway mapping analysis revealed that the activation of gonadotropin-releasing hormone signaling, calcium signaling, and progesterone-mediated oocyte maturation pathways were putatively crucial to ovarian maturation induced by the ablation. These findings shed light on several possible molecular mechanisms of the eyestalk ablation effect and allow more focused investigation for an ultimate goal of finding alternative methods to replace the undesirable practice of the eyestalk ablation in the future. |
| format |
article |
| author |
Umaporn Uawisetwathana Rungnapa Leelatanawit Amornpan Klanchui Juthatip Prommoon Sirawut Klinbunga Nitsara Karoonuthaisiri |
| author_facet |
Umaporn Uawisetwathana Rungnapa Leelatanawit Amornpan Klanchui Juthatip Prommoon Sirawut Klinbunga Nitsara Karoonuthaisiri |
| author_sort |
Umaporn Uawisetwathana |
| title |
Insights into eyestalk ablation mechanism to induce ovarian maturation in the black tiger shrimp. |
| title_short |
Insights into eyestalk ablation mechanism to induce ovarian maturation in the black tiger shrimp. |
| title_full |
Insights into eyestalk ablation mechanism to induce ovarian maturation in the black tiger shrimp. |
| title_fullStr |
Insights into eyestalk ablation mechanism to induce ovarian maturation in the black tiger shrimp. |
| title_full_unstemmed |
Insights into eyestalk ablation mechanism to induce ovarian maturation in the black tiger shrimp. |
| title_sort |
insights into eyestalk ablation mechanism to induce ovarian maturation in the black tiger shrimp. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/6016aafac90e42c9ad537248ed1342cc |
| work_keys_str_mv |
AT umapornuawisetwathana insightsintoeyestalkablationmechanismtoinduceovarianmaturationintheblacktigershrimp AT rungnapaleelatanawit insightsintoeyestalkablationmechanismtoinduceovarianmaturationintheblacktigershrimp AT amornpanklanchui insightsintoeyestalkablationmechanismtoinduceovarianmaturationintheblacktigershrimp AT juthatipprommoon insightsintoeyestalkablationmechanismtoinduceovarianmaturationintheblacktigershrimp AT sirawutklinbunga insightsintoeyestalkablationmechanismtoinduceovarianmaturationintheblacktigershrimp AT nitsarakaroonuthaisiri insightsintoeyestalkablationmechanismtoinduceovarianmaturationintheblacktigershrimp |
| _version_ |
1718424418086027264 |