Oxidative stress induced in sunflower seedling roots by aqueous dry olive-mill residues.

The contamination of soils with dry olive-mill residue can represent a serious problem as being an environmental stressor in plants. It has been demonstrated that inoculation of aqueous extract of olive oil-mill residue (ADOR) with saprobe fungi removes some phenolic compounds. In this paper we stud...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Inmaculada Garrido, Mercedes García-Sánchez, Ilda Casimiro, Pedro Joaquin Casero, Inmaculada García-Romera, Juan Antonio Ocampo, Francisco Espinosa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/601f7926bc85418e8f557b9b434b3c6e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:601f7926bc85418e8f557b9b434b3c6e
record_format	dspace
spelling	oai:doaj.org-article:601f7926bc85418e8f557b9b434b3c6e2021-11-18T08:13:51ZOxidative stress induced in sunflower seedling roots by aqueous dry olive-mill residues.1932-620310.1371/journal.pone.0046137https://doaj.org/article/601f7926bc85418e8f557b9b434b3c6e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23049960/?tool=EBIhttps://doaj.org/toc/1932-6203The contamination of soils with dry olive-mill residue can represent a serious problem as being an environmental stressor in plants. It has been demonstrated that inoculation of aqueous extract of olive oil-mill residue (ADOR) with saprobe fungi removes some phenolic compounds. In this paper we studied the effect of ADOR uninoculated or inoculated with saprobe fungi in sunflower seedling roots. The germination and root growth, O(2)·(-) generation, superoxide dismutase (SOD) and extracellular peroxidases (EC-POXs) activities, and the content of some metabolites involved in the tolerance of stress were tested. The roots germinated in ADOR uninoculated show a decrease in meristem size, resulting in a reduction of the root length and fresh weight, and in the number of layers forming the cortex, but did not alter the dry weight, protein and soluble amino acid content. ADOR caused the decreases in O(2)·(-) generation and EC-POX's activities and protein oxidation, but enhanced SOD activity, lipid peroxidation and proline content. Fluorescence imaging showed that ADOR induced O(2)·(-) and H(2)O(2) accumulation in the roots. The increase in SOD and the decrease in EC-POX's activities might be involved in the enhancement of H(2)O(2) content and lipid peroxidation. Control roots treated with ADOR for 10 min show an oxidative burst. Roots germinated in ADOR inoculated with saprobe fungi partially recovered normal levels of ROS, morphological characteristics and antioxidant activities. These results suggested that treatment with ADOR caused a phytotoxic effect during germination inducing an oxidative stress. The inoculation of ADOR with saprobe fungi limited the stress.Inmaculada GarridoMercedes García-SánchezIlda CasimiroPedro Joaquin CaseroInmaculada García-RomeraJuan Antonio OcampoFrancisco EspinosaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e46137 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Inmaculada Garrido Mercedes García-Sánchez Ilda Casimiro Pedro Joaquin Casero Inmaculada García-Romera Juan Antonio Ocampo Francisco Espinosa Oxidative stress induced in sunflower seedling roots by aqueous dry olive-mill residues.
description	The contamination of soils with dry olive-mill residue can represent a serious problem as being an environmental stressor in plants. It has been demonstrated that inoculation of aqueous extract of olive oil-mill residue (ADOR) with saprobe fungi removes some phenolic compounds. In this paper we studied the effect of ADOR uninoculated or inoculated with saprobe fungi in sunflower seedling roots. The germination and root growth, O(2)·(-) generation, superoxide dismutase (SOD) and extracellular peroxidases (EC-POXs) activities, and the content of some metabolites involved in the tolerance of stress were tested. The roots germinated in ADOR uninoculated show a decrease in meristem size, resulting in a reduction of the root length and fresh weight, and in the number of layers forming the cortex, but did not alter the dry weight, protein and soluble amino acid content. ADOR caused the decreases in O(2)·(-) generation and EC-POX's activities and protein oxidation, but enhanced SOD activity, lipid peroxidation and proline content. Fluorescence imaging showed that ADOR induced O(2)·(-) and H(2)O(2) accumulation in the roots. The increase in SOD and the decrease in EC-POX's activities might be involved in the enhancement of H(2)O(2) content and lipid peroxidation. Control roots treated with ADOR for 10 min show an oxidative burst. Roots germinated in ADOR inoculated with saprobe fungi partially recovered normal levels of ROS, morphological characteristics and antioxidant activities. These results suggested that treatment with ADOR caused a phytotoxic effect during germination inducing an oxidative stress. The inoculation of ADOR with saprobe fungi limited the stress.
format	article
author	Inmaculada Garrido Mercedes García-Sánchez Ilda Casimiro Pedro Joaquin Casero Inmaculada García-Romera Juan Antonio Ocampo Francisco Espinosa
author_facet	Inmaculada Garrido Mercedes García-Sánchez Ilda Casimiro Pedro Joaquin Casero Inmaculada García-Romera Juan Antonio Ocampo Francisco Espinosa
author_sort	Inmaculada Garrido
title	Oxidative stress induced in sunflower seedling roots by aqueous dry olive-mill residues.
title_short	Oxidative stress induced in sunflower seedling roots by aqueous dry olive-mill residues.
title_full	Oxidative stress induced in sunflower seedling roots by aqueous dry olive-mill residues.
title_fullStr	Oxidative stress induced in sunflower seedling roots by aqueous dry olive-mill residues.
title_full_unstemmed	Oxidative stress induced in sunflower seedling roots by aqueous dry olive-mill residues.
title_sort	oxidative stress induced in sunflower seedling roots by aqueous dry olive-mill residues.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/601f7926bc85418e8f557b9b434b3c6e
work_keys_str_mv	AT inmaculadagarrido oxidativestressinducedinsunflowerseedlingrootsbyaqueousdryolivemillresidues AT mercedesgarciasanchez oxidativestressinducedinsunflowerseedlingrootsbyaqueousdryolivemillresidues AT ildacasimiro oxidativestressinducedinsunflowerseedlingrootsbyaqueousdryolivemillresidues AT pedrojoaquincasero oxidativestressinducedinsunflowerseedlingrootsbyaqueousdryolivemillresidues AT inmaculadagarciaromera oxidativestressinducedinsunflowerseedlingrootsbyaqueousdryolivemillresidues AT juanantonioocampo oxidativestressinducedinsunflowerseedlingrootsbyaqueousdryolivemillresidues AT franciscoespinosa oxidativestressinducedinsunflowerseedlingrootsbyaqueousdryolivemillresidues
_version_	1718422045675487232

Oxidative stress induced in sunflower seedling roots by aqueous dry olive-mill residues.

Ejemplares similares