Protein supplementation during an energy-restricted diet induces visceral fat loss and gut microbiota amino acid metabolism activation: a randomized trial

Abstract Interactions between diet and gut microbiota are critical regulators of energy metabolism. The effects of fibre intake have been deeply studied but little is known about the impact of proteins. Here, we investigated the effects of high protein supplementation (Investigational Product, IP) i...

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Autores principales:	Pierre Bel Lassen, Eugeni Belda, Edi Prifti, Maria Carlota Dao, Florian Specque, Corneliu Henegar, Laure Rinaldi, Xuedan Wang, Sean P. Kennedy, Jean-Daniel Zucker, Wim Calame, Benoît Lamarche, Sandrine P. Claus, Karine Clément
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/60260b2442fa442b9a8a5900f4157062
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spelling	oai:doaj.org-article:60260b2442fa442b9a8a5900f41570622021-12-02T16:35:36ZProtein supplementation during an energy-restricted diet induces visceral fat loss and gut microbiota amino acid metabolism activation: a randomized trial10.1038/s41598-021-94916-92045-2322https://doaj.org/article/60260b2442fa442b9a8a5900f41570622021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94916-9https://doaj.org/toc/2045-2322Abstract Interactions between diet and gut microbiota are critical regulators of energy metabolism. The effects of fibre intake have been deeply studied but little is known about the impact of proteins. Here, we investigated the effects of high protein supplementation (Investigational Product, IP) in a double blind, randomised placebo-controled intervention study (NCT01755104) where 107 participants received the IP or an isocaloric normoproteic comparator (CP) alongside a mild caloric restriction. Gut microbiota profiles were explored in a patient subset (n = 53) using shotgun metagenomic sequencing. Visceral fat decreased in both groups (IP group: − 20.8 ± 23.2 cm2; CP group: − 14.5 ± 24.3 cm2) with a greater reduction (p < 0.05) with the IP supplementation in the Per Protocol population. Microbial diversity increased in individuals with a baseline low gene count (p < 0.05). The decrease in weight, fat mass and visceral fat mass significantly correlated with the increase in microbial diversity (p < 0.05). Protein supplementation had little effects on bacteria composition but major differences were seen at functional level. Protein supplementation stimulated bacterial amino acid metabolism (90% amino-acid synthesis functions enriched with IP versus 13% in CP group (p < 0.01)). Protein supplementation alongside a mild energy restriction induces visceral fat mass loss and an activation of gut microbiota amino-acid metabolism. Clinical trial registration: NCT01755104 (24/12/2012). https://clinicaltrials.gov/ct2/show/record/NCT01755104?term=NCT01755104&draw=2&rank=1 .Pierre Bel LassenEugeni BeldaEdi PriftiMaria Carlota DaoFlorian SpecqueCorneliu HenegarLaure RinaldiXuedan WangSean P. KennedyJean-Daniel ZuckerWim CalameBenoît LamarcheSandrine P. ClausKarine ClémentNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pierre Bel Lassen Eugeni Belda Edi Prifti Maria Carlota Dao Florian Specque Corneliu Henegar Laure Rinaldi Xuedan Wang Sean P. Kennedy Jean-Daniel Zucker Wim Calame Benoît Lamarche Sandrine P. Claus Karine Clément Protein supplementation during an energy-restricted diet induces visceral fat loss and gut microbiota amino acid metabolism activation: a randomized trial
description	Abstract Interactions between diet and gut microbiota are critical regulators of energy metabolism. The effects of fibre intake have been deeply studied but little is known about the impact of proteins. Here, we investigated the effects of high protein supplementation (Investigational Product, IP) in a double blind, randomised placebo-controled intervention study (NCT01755104) where 107 participants received the IP or an isocaloric normoproteic comparator (CP) alongside a mild caloric restriction. Gut microbiota profiles were explored in a patient subset (n = 53) using shotgun metagenomic sequencing. Visceral fat decreased in both groups (IP group: − 20.8 ± 23.2 cm2; CP group: − 14.5 ± 24.3 cm2) with a greater reduction (p < 0.05) with the IP supplementation in the Per Protocol population. Microbial diversity increased in individuals with a baseline low gene count (p < 0.05). The decrease in weight, fat mass and visceral fat mass significantly correlated with the increase in microbial diversity (p < 0.05). Protein supplementation had little effects on bacteria composition but major differences were seen at functional level. Protein supplementation stimulated bacterial amino acid metabolism (90% amino-acid synthesis functions enriched with IP versus 13% in CP group (p < 0.01)). Protein supplementation alongside a mild energy restriction induces visceral fat mass loss and an activation of gut microbiota amino-acid metabolism. Clinical trial registration: NCT01755104 (24/12/2012). https://clinicaltrials.gov/ct2/show/record/NCT01755104?term=NCT01755104&draw=2&rank=1 .
format	article
author	Pierre Bel Lassen Eugeni Belda Edi Prifti Maria Carlota Dao Florian Specque Corneliu Henegar Laure Rinaldi Xuedan Wang Sean P. Kennedy Jean-Daniel Zucker Wim Calame Benoît Lamarche Sandrine P. Claus Karine Clément
author_facet	Pierre Bel Lassen Eugeni Belda Edi Prifti Maria Carlota Dao Florian Specque Corneliu Henegar Laure Rinaldi Xuedan Wang Sean P. Kennedy Jean-Daniel Zucker Wim Calame Benoît Lamarche Sandrine P. Claus Karine Clément
author_sort	Pierre Bel Lassen
title	Protein supplementation during an energy-restricted diet induces visceral fat loss and gut microbiota amino acid metabolism activation: a randomized trial
title_short	Protein supplementation during an energy-restricted diet induces visceral fat loss and gut microbiota amino acid metabolism activation: a randomized trial
title_full	Protein supplementation during an energy-restricted diet induces visceral fat loss and gut microbiota amino acid metabolism activation: a randomized trial
title_fullStr	Protein supplementation during an energy-restricted diet induces visceral fat loss and gut microbiota amino acid metabolism activation: a randomized trial
title_full_unstemmed	Protein supplementation during an energy-restricted diet induces visceral fat loss and gut microbiota amino acid metabolism activation: a randomized trial
title_sort	protein supplementation during an energy-restricted diet induces visceral fat loss and gut microbiota amino acid metabolism activation: a randomized trial
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/60260b2442fa442b9a8a5900f4157062
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Protein supplementation during an energy-restricted diet induces visceral fat loss and gut microbiota amino acid metabolism activation: a randomized trial

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