Evolution of Th2 responses: characterization of IL-4/13 in sea bass (Dicentrarchus labrax L.) and studies of expression and biological activity

Abstract Th2 immunity is a primary host defence against metazoan pathogens and two of the important cytokines involved in this immune response in mammals are IL-4 and IL-13. Recently the origin and evolution of Th2 immune responses have been investigated in fish where a molecule with relatedness to...

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Autores principales:	Valentina Stocchi, Tiehui Wang, Elisa Randelli, Massimo Mazzini, Marco Gerdol, Alberto Pallavicini, Chris J. Secombes, Giuseppe Scapigliati, Francesco Buonocore
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/602984e3601a48adb62dec12b66699c3
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spelling	oai:doaj.org-article:602984e3601a48adb62dec12b66699c32021-12-02T15:05:30ZEvolution of Th2 responses: characterization of IL-4/13 in sea bass (Dicentrarchus labrax L.) and studies of expression and biological activity10.1038/s41598-017-02472-y2045-2322https://doaj.org/article/602984e3601a48adb62dec12b66699c32017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02472-yhttps://doaj.org/toc/2045-2322Abstract Th2 immunity is a primary host defence against metazoan pathogens and two of the important cytokines involved in this immune response in mammals are IL-4 and IL-13. Recently the origin and evolution of Th2 immune responses have been investigated in fish where a molecule with relatedness to both IL-4 and IL-13 is present, termed IL-4/13. Different IL-4/13 paralogues (IL-4/13 A and IL-4/13B) exist in teleost fish. In this paper, we have focused on the IL-4/13 isoforms found in the European sea bass (Dicentrarchus labrax L.). Two tandem duplicated but divergent IL-4/13 A isoforms and one IL-4/13B are present, a unique situation compared to other teleosts. These genes were studied in terms of their in vitro and in vivo transcript levels after different treatments and their biological activities after production of the recombinant isoforms. The results show that the presence of these three paralogues is associated with different activities, both in terms of their expression profiles and the ability of the proteins to modulate the expression of immune genes in head kidney leukocytes. It is clear that the initiation and control of type-2 responses in seabass is complex due to the presence of multiple IL-4/13 isoforms with overlapping but distinct activities.Valentina StocchiTiehui WangElisa RandelliMassimo MazziniMarco GerdolAlberto PallaviciniChris J. SecombesGiuseppe ScapigliatiFrancesco BuonocoreNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valentina Stocchi Tiehui Wang Elisa Randelli Massimo Mazzini Marco Gerdol Alberto Pallavicini Chris J. Secombes Giuseppe Scapigliati Francesco Buonocore Evolution of Th2 responses: characterization of IL-4/13 in sea bass (Dicentrarchus labrax L.) and studies of expression and biological activity
description	Abstract Th2 immunity is a primary host defence against metazoan pathogens and two of the important cytokines involved in this immune response in mammals are IL-4 and IL-13. Recently the origin and evolution of Th2 immune responses have been investigated in fish where a molecule with relatedness to both IL-4 and IL-13 is present, termed IL-4/13. Different IL-4/13 paralogues (IL-4/13 A and IL-4/13B) exist in teleost fish. In this paper, we have focused on the IL-4/13 isoforms found in the European sea bass (Dicentrarchus labrax L.). Two tandem duplicated but divergent IL-4/13 A isoforms and one IL-4/13B are present, a unique situation compared to other teleosts. These genes were studied in terms of their in vitro and in vivo transcript levels after different treatments and their biological activities after production of the recombinant isoforms. The results show that the presence of these three paralogues is associated with different activities, both in terms of their expression profiles and the ability of the proteins to modulate the expression of immune genes in head kidney leukocytes. It is clear that the initiation and control of type-2 responses in seabass is complex due to the presence of multiple IL-4/13 isoforms with overlapping but distinct activities.
format	article
author	Valentina Stocchi Tiehui Wang Elisa Randelli Massimo Mazzini Marco Gerdol Alberto Pallavicini Chris J. Secombes Giuseppe Scapigliati Francesco Buonocore
author_facet	Valentina Stocchi Tiehui Wang Elisa Randelli Massimo Mazzini Marco Gerdol Alberto Pallavicini Chris J. Secombes Giuseppe Scapigliati Francesco Buonocore
author_sort	Valentina Stocchi
title	Evolution of Th2 responses: characterization of IL-4/13 in sea bass (Dicentrarchus labrax L.) and studies of expression and biological activity
title_short	Evolution of Th2 responses: characterization of IL-4/13 in sea bass (Dicentrarchus labrax L.) and studies of expression and biological activity
title_full	Evolution of Th2 responses: characterization of IL-4/13 in sea bass (Dicentrarchus labrax L.) and studies of expression and biological activity
title_fullStr	Evolution of Th2 responses: characterization of IL-4/13 in sea bass (Dicentrarchus labrax L.) and studies of expression and biological activity
title_full_unstemmed	Evolution of Th2 responses: characterization of IL-4/13 in sea bass (Dicentrarchus labrax L.) and studies of expression and biological activity
title_sort	evolution of th2 responses: characterization of il-4/13 in sea bass (dicentrarchus labrax l.) and studies of expression and biological activity
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/602984e3601a48adb62dec12b66699c3
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Evolution of Th2 responses: characterization of IL-4/13 in sea bass (Dicentrarchus labrax L.) and studies of expression and biological activity

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