Genome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori.

Polycomb group (PcG) proteins are evolutionarily conserved chromatin modifiers and act together in three multimeric complexes, Polycomb repressive complex 1 (PRC1), Polycomb repressive complex 2 (PRC2), and Pleiohomeotic repressive complex (PhoRC), to repress transcription of the target genes. Here,...

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Autores principales:	Zhiqing Li, Daojun Cheng, Hiroaki Mon, Tsuneyuki Tatsuke, Li Zhu, Jian Xu, Jae Man Lee, Qingyou Xia, Takahiro Kusakabe
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6030881e09074737b3c363a520ad58b0
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spelling	oai:doaj.org-article:6030881e09074737b3c363a520ad58b02021-11-18T07:23:28ZGenome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori.1932-620310.1371/journal.pone.0034330https://doaj.org/article/6030881e09074737b3c363a520ad58b02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22485166/?tool=EBIhttps://doaj.org/toc/1932-6203Polycomb group (PcG) proteins are evolutionarily conserved chromatin modifiers and act together in three multimeric complexes, Polycomb repressive complex 1 (PRC1), Polycomb repressive complex 2 (PRC2), and Pleiohomeotic repressive complex (PhoRC), to repress transcription of the target genes. Here, we identified Polycomb target genes in Bombyx mori with holocentric centromere using genome-wide expression screening based on the knockdown of BmSCE, BmESC, BmPHO, or BmSCM gene, which represent the distinct complexes. As a result, the expressions of 29 genes were up-regulated after knocking down 4 PcG genes. Particularly, there is a significant overlap between targets of BmPho (331 out of 524) and BmScm (331 out of 532), and among these, 190 genes function as regulator factors playing important roles in development. We also found that BmPho, as well as BmScm, can interact with other Polycomb components examined in this study. Further detailed analysis revealed that the C-terminus of BmPho containing zinc finger domain is involved in the interaction between BmPho and BmScm. Moreover, the zinc finger domain in BmPho contributes to its inhibitory function and ectopic overexpression of BmScm is able to promote transcriptional repression by Gal4-Pho fusions including BmScm-interacting domain. Loss of BmPho expression causes relocalization of BmScm into the cytoplasm. Collectively, we provide evidence of a functional link between BmPho and BmScm, and propose two Polycomb-related repression mechanisms requiring only BmPho associated with BmScm or a whole set of PcG complexes.Zhiqing LiDaojun ChengHiroaki MonTsuneyuki TatsukeLi ZhuJian XuJae Man LeeQingyou XiaTakahiro KusakabePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e34330 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhiqing Li Daojun Cheng Hiroaki Mon Tsuneyuki Tatsuke Li Zhu Jian Xu Jae Man Lee Qingyou Xia Takahiro Kusakabe Genome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori.
description	Polycomb group (PcG) proteins are evolutionarily conserved chromatin modifiers and act together in three multimeric complexes, Polycomb repressive complex 1 (PRC1), Polycomb repressive complex 2 (PRC2), and Pleiohomeotic repressive complex (PhoRC), to repress transcription of the target genes. Here, we identified Polycomb target genes in Bombyx mori with holocentric centromere using genome-wide expression screening based on the knockdown of BmSCE, BmESC, BmPHO, or BmSCM gene, which represent the distinct complexes. As a result, the expressions of 29 genes were up-regulated after knocking down 4 PcG genes. Particularly, there is a significant overlap between targets of BmPho (331 out of 524) and BmScm (331 out of 532), and among these, 190 genes function as regulator factors playing important roles in development. We also found that BmPho, as well as BmScm, can interact with other Polycomb components examined in this study. Further detailed analysis revealed that the C-terminus of BmPho containing zinc finger domain is involved in the interaction between BmPho and BmScm. Moreover, the zinc finger domain in BmPho contributes to its inhibitory function and ectopic overexpression of BmScm is able to promote transcriptional repression by Gal4-Pho fusions including BmScm-interacting domain. Loss of BmPho expression causes relocalization of BmScm into the cytoplasm. Collectively, we provide evidence of a functional link between BmPho and BmScm, and propose two Polycomb-related repression mechanisms requiring only BmPho associated with BmScm or a whole set of PcG complexes.
format	article
author	Zhiqing Li Daojun Cheng Hiroaki Mon Tsuneyuki Tatsuke Li Zhu Jian Xu Jae Man Lee Qingyou Xia Takahiro Kusakabe
author_facet	Zhiqing Li Daojun Cheng Hiroaki Mon Tsuneyuki Tatsuke Li Zhu Jian Xu Jae Man Lee Qingyou Xia Takahiro Kusakabe
author_sort	Zhiqing Li
title	Genome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori.
title_short	Genome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori.
title_full	Genome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori.
title_fullStr	Genome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori.
title_full_unstemmed	Genome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori.
title_sort	genome-wide identification of polycomb target genes reveals a functional association of pho with scm in bombyx mori.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6030881e09074737b3c363a520ad58b0
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Genome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori.

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