Isolation, Detection, and Quantification of Cancer Biomarkers in HPV-Associated Malignancies

Abstract Human Papillomavirus (HPV) infection has been recognized as the main etiologic factor in the development of various cancers including penile, vulva, oropharyngeal and cervical cancers. In the development of cancer, persistent HPV infections induce E6 and E7 oncoproteins, which promote cell...

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Autores principales:	Hakan Inan, Shuqi Wang, Fatih Inci, Murat Baday, Richard Zangar, Sailaja Kesiraju, Karen S. Anderson, Brian T. Cunningham, Utkan Demirci
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/60421e379109442d8308306482a0ac8d
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spelling	oai:doaj.org-article:60421e379109442d8308306482a0ac8d2021-12-02T16:07:44ZIsolation, Detection, and Quantification of Cancer Biomarkers in HPV-Associated Malignancies10.1038/s41598-017-02672-62045-2322https://doaj.org/article/60421e379109442d8308306482a0ac8d2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02672-6https://doaj.org/toc/2045-2322Abstract Human Papillomavirus (HPV) infection has been recognized as the main etiologic factor in the development of various cancers including penile, vulva, oropharyngeal and cervical cancers. In the development of cancer, persistent HPV infections induce E6 and E7 oncoproteins, which promote cell proliferation and carcinogenesis resulting elevated levels of host antibodies (e.g., anti-HPV16 E7 antibody). Currently, these cancers are clinically diagnosed using invasive biopsy-based tests, which are performed only in centralized labs by experienced clinical staff using time-consuming and expensive tools and technologies. Therefore, these obstacles constrain their utilization at primary care clinics and in remote settings, where resources are limited. Here, we present a rapid, inexpensive, reliable, easy-to-use, customized immunoassay platform following a microfluidic filter device to detect and quantify anti-HPV16 E7 antibodies from whole blood as a non-invasive assisting technology for diagnosis of HPV-associated malignancies, especially, at primary healthcare and remote settings. The platform can detect and quantify anti-HPV16 E7 antibody down to 2.87 ng/mL. We further validated our immunoassay in clinical patient samples and it provided significantly high responses as compared to control samples. Thus, it can be potentially implemented as a pretesting tool to identify high-risk groups for broad monitoring of HPV-associated cancers in resource-constrained settings.Hakan InanShuqi WangFatih InciMurat BadayRichard ZangarSailaja KesirajuKaren S. AndersonBrian T. CunninghamUtkan DemirciNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hakan Inan Shuqi Wang Fatih Inci Murat Baday Richard Zangar Sailaja Kesiraju Karen S. Anderson Brian T. Cunningham Utkan Demirci Isolation, Detection, and Quantification of Cancer Biomarkers in HPV-Associated Malignancies
description	Abstract Human Papillomavirus (HPV) infection has been recognized as the main etiologic factor in the development of various cancers including penile, vulva, oropharyngeal and cervical cancers. In the development of cancer, persistent HPV infections induce E6 and E7 oncoproteins, which promote cell proliferation and carcinogenesis resulting elevated levels of host antibodies (e.g., anti-HPV16 E7 antibody). Currently, these cancers are clinically diagnosed using invasive biopsy-based tests, which are performed only in centralized labs by experienced clinical staff using time-consuming and expensive tools and technologies. Therefore, these obstacles constrain their utilization at primary care clinics and in remote settings, where resources are limited. Here, we present a rapid, inexpensive, reliable, easy-to-use, customized immunoassay platform following a microfluidic filter device to detect and quantify anti-HPV16 E7 antibodies from whole blood as a non-invasive assisting technology for diagnosis of HPV-associated malignancies, especially, at primary healthcare and remote settings. The platform can detect and quantify anti-HPV16 E7 antibody down to 2.87 ng/mL. We further validated our immunoassay in clinical patient samples and it provided significantly high responses as compared to control samples. Thus, it can be potentially implemented as a pretesting tool to identify high-risk groups for broad monitoring of HPV-associated cancers in resource-constrained settings.
format	article
author	Hakan Inan Shuqi Wang Fatih Inci Murat Baday Richard Zangar Sailaja Kesiraju Karen S. Anderson Brian T. Cunningham Utkan Demirci
author_facet	Hakan Inan Shuqi Wang Fatih Inci Murat Baday Richard Zangar Sailaja Kesiraju Karen S. Anderson Brian T. Cunningham Utkan Demirci
author_sort	Hakan Inan
title	Isolation, Detection, and Quantification of Cancer Biomarkers in HPV-Associated Malignancies
title_short	Isolation, Detection, and Quantification of Cancer Biomarkers in HPV-Associated Malignancies
title_full	Isolation, Detection, and Quantification of Cancer Biomarkers in HPV-Associated Malignancies
title_fullStr	Isolation, Detection, and Quantification of Cancer Biomarkers in HPV-Associated Malignancies
title_full_unstemmed	Isolation, Detection, and Quantification of Cancer Biomarkers in HPV-Associated Malignancies
title_sort	isolation, detection, and quantification of cancer biomarkers in hpv-associated malignancies
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/60421e379109442d8308306482a0ac8d
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Isolation, Detection, and Quantification of Cancer Biomarkers in HPV-Associated Malignancies

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