Antibacterial, anti-inflammatory and peroxidase-mediated cyclooxygenase-1 inhibitory properties of Fusarium solani extract

Context: Nigerian soil fungi population is unexplored. It is hypothesized that they harbour new bioactive chemicals. This hypothesis is based on the large percentage of currently approved medicines that originated from soil-inhabiting micro-organisms Objectives: To investigate the antimicrobial and...

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Autores principales:	Kenneth Ngwoke, Nwalusiuka Tochukwu, Chinechem Ekwealor, Valerie Nwankwo, Uju Obi-Okafor, Chisom Izundu, Festus B. C. Okoye, Charles Esimone, Peter Proksch, Chen Situ
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	cox-1 mic inflammation soil fungi pathogens cancer Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/604531ed779e477a886ef94697f289a6
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spelling	oai:doaj.org-article:604531ed779e477a886ef94697f289a62021-11-17T14:21:56ZAntibacterial, anti-inflammatory and peroxidase-mediated cyclooxygenase-1 inhibitory properties of Fusarium solani extract1388-02091744-511610.1080/13880209.2019.1606260https://doaj.org/article/604531ed779e477a886ef94697f289a62019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1606260https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Nigerian soil fungi population is unexplored. It is hypothesized that they harbour new bioactive chemicals. This hypothesis is based on the large percentage of currently approved medicines that originated from soil-inhabiting micro-organisms Objectives: To investigate the antimicrobial and anti-inflammatory properties of Fusarium solani ethyl acetate (EtOAc) extract selected based on its broad spectrum of antimicrobial potential in an overlay experiment with seven other soil fungi strains. Materials and methods: Fungus number 6 (F6), identified by molecular characterization as Fusarium solani (Mart.) Sacc (Nectriaceae) was selected for studies from eight purified soil fungi due to its superior broad-spectrum antibiotics producing potential following agar overlay experiment. F6 was fermented for 21 d and the minimum inhibitory concentration (MIC) of its EtOAc fermentation extract (dose range: 12.5–100 µg/mL) was determined using agar dilution method for Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and anti-inflammatory properties determined using rat-paw (250–500 mg/kg) and xylene induced oedema (250–500 µg/kg) (in Swiss albino rats and mice) models, respectively. The ability of the extract to inhibit cyclooxygenase (COX) enzyme was also determined in vitro using Cayman test kit-760111. Result: The MIC of the EtOAc extract was <12.5 µg/mL for S. aureus, P. aeruginosa and Escherichia coli. It inhibited xylene induced oedema by 65% compared with 61% observed for diclofenac and was significantly (p < 0.05) better than diclofenac in rat-paw-oedema model within the first phase of inflammation. The extract inhibited COX-1 peroxidase-mediated activities with an IC50 below 5 µg/mL. Conclusions: The extract exhibited strong antibacterial and anti-inflammatory properties, warranting further investigations into therapeutic potential of this fungus. This study design can be adapted in soil fungi metabolomic investigations. We report for the first time the potent anti-inflammatory property of the ethyl acetate extract of soil strain of F. solani with a possible mechanism of action that involves the inhibition of COX enzyme.Kenneth NgwokeNwalusiuka TochukwuChinechem EkwealorValerie NwankwoUju Obi-OkaforChisom IzunduFestus B. C. OkoyeCharles EsimonePeter ProkschChen SituTaylor & Francis Grouparticlecox-1micinflammationsoil fungipathogenscancer Therapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 310-317 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cox-1 mic inflammation soil fungi pathogens cancer Therapeutics. Pharmacology RM1-950
spellingShingle	cox-1 mic inflammation soil fungi pathogens cancer Therapeutics. Pharmacology RM1-950 Kenneth Ngwoke Nwalusiuka Tochukwu Chinechem Ekwealor Valerie Nwankwo Uju Obi-Okafor Chisom Izundu Festus B. C. Okoye Charles Esimone Peter Proksch Chen Situ Antibacterial, anti-inflammatory and peroxidase-mediated cyclooxygenase-1 inhibitory properties of Fusarium solani extract
description	Context: Nigerian soil fungi population is unexplored. It is hypothesized that they harbour new bioactive chemicals. This hypothesis is based on the large percentage of currently approved medicines that originated from soil-inhabiting micro-organisms Objectives: To investigate the antimicrobial and anti-inflammatory properties of Fusarium solani ethyl acetate (EtOAc) extract selected based on its broad spectrum of antimicrobial potential in an overlay experiment with seven other soil fungi strains. Materials and methods: Fungus number 6 (F6), identified by molecular characterization as Fusarium solani (Mart.) Sacc (Nectriaceae) was selected for studies from eight purified soil fungi due to its superior broad-spectrum antibiotics producing potential following agar overlay experiment. F6 was fermented for 21 d and the minimum inhibitory concentration (MIC) of its EtOAc fermentation extract (dose range: 12.5–100 µg/mL) was determined using agar dilution method for Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and anti-inflammatory properties determined using rat-paw (250–500 mg/kg) and xylene induced oedema (250–500 µg/kg) (in Swiss albino rats and mice) models, respectively. The ability of the extract to inhibit cyclooxygenase (COX) enzyme was also determined in vitro using Cayman test kit-760111. Result: The MIC of the EtOAc extract was <12.5 µg/mL for S. aureus, P. aeruginosa and Escherichia coli. It inhibited xylene induced oedema by 65% compared with 61% observed for diclofenac and was significantly (p < 0.05) better than diclofenac in rat-paw-oedema model within the first phase of inflammation. The extract inhibited COX-1 peroxidase-mediated activities with an IC50 below 5 µg/mL. Conclusions: The extract exhibited strong antibacterial and anti-inflammatory properties, warranting further investigations into therapeutic potential of this fungus. This study design can be adapted in soil fungi metabolomic investigations. We report for the first time the potent anti-inflammatory property of the ethyl acetate extract of soil strain of F. solani with a possible mechanism of action that involves the inhibition of COX enzyme.
format	article
author	Kenneth Ngwoke Nwalusiuka Tochukwu Chinechem Ekwealor Valerie Nwankwo Uju Obi-Okafor Chisom Izundu Festus B. C. Okoye Charles Esimone Peter Proksch Chen Situ
author_facet	Kenneth Ngwoke Nwalusiuka Tochukwu Chinechem Ekwealor Valerie Nwankwo Uju Obi-Okafor Chisom Izundu Festus B. C. Okoye Charles Esimone Peter Proksch Chen Situ
author_sort	Kenneth Ngwoke
title	Antibacterial, anti-inflammatory and peroxidase-mediated cyclooxygenase-1 inhibitory properties of Fusarium solani extract
title_short	Antibacterial, anti-inflammatory and peroxidase-mediated cyclooxygenase-1 inhibitory properties of Fusarium solani extract
title_full	Antibacterial, anti-inflammatory and peroxidase-mediated cyclooxygenase-1 inhibitory properties of Fusarium solani extract
title_fullStr	Antibacterial, anti-inflammatory and peroxidase-mediated cyclooxygenase-1 inhibitory properties of Fusarium solani extract
title_full_unstemmed	Antibacterial, anti-inflammatory and peroxidase-mediated cyclooxygenase-1 inhibitory properties of Fusarium solani extract
title_sort	antibacterial, anti-inflammatory and peroxidase-mediated cyclooxygenase-1 inhibitory properties of fusarium solani extract
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/604531ed779e477a886ef94697f289a6
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Antibacterial, anti-inflammatory and peroxidase-mediated cyclooxygenase-1 inhibitory properties of Fusarium solani extract

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