High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense

Raquel Rangel Silvares1,*, Evelyn Nunes Goulart da Silva Pereira1,*, Edgar Eduardo Ilaquita Flores1, Karine Lino Rodrigues1, Adriana Ribeiro Silva2, Cassiano Felipe Gonçalves-de-Albuquerque2,3, Anissa Daliry1 1Laboratory of Cardiovascular Investigation, Oswaldo Cruz Institute, Oswaldo Cru...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rangel Silvares R, Nunes Goulart da Silva Pereira E, Eduardo Ilaquita Flores E, Lino Rodrigues K, Ribeiro Silva A, Gonçalves-de-Albuquerque CF, Daliry A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Metabolic syndrome kidney endothelial dysfunction pyridoxamine advanced glycation end products. Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/605b442423bd452da7f8a6a54ce3800f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:605b442423bd452da7f8a6a54ce3800f
record_format	dspace
spelling	oai:doaj.org-article:605b442423bd452da7f8a6a54ce3800f2021-12-02T09:06:08ZHigh-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense1178-7007https://doaj.org/article/605b442423bd452da7f8a6a54ce3800f2019-09-01T00:00:00Zhttps://www.dovepress.com/high-fat-diet-induced-kidney-alterations-in-rats-with-metabolic-syndro-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Raquel Rangel Silvares1,, Evelyn Nunes Goulart da Silva Pereira1,, Edgar Eduardo Ilaquita Flores1, Karine Lino Rodrigues1, Adriana Ribeiro Silva2, Cassiano Felipe Gonçalves-de-Albuquerque2,3, Anissa Daliry1 1Laboratory of Cardiovascular Investigation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil; 2Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil; 3Laboratory of Immunopharmacology, Federal University of the State of Rio de Janeiro, Rio de Janeiro, RJ, Brazil*These authors contributed equally to this workCorrespondence: Anissa DaliryInstituto Oswaldo Cruz, Fiocruz, Pavilhão Ozório de Almeida, Manguinhos, Rio de Janeiro, CEP: 21.040-360, RJ, BrazilEmail daliry@ioc.fiocruz.brIntroduction: This study aimed to investigate changes in renal function and the AGE-RAGE axis in the kidney of a non-genetic animal model of metabolic syndrome (MetS) induced by high-fat diet (HFD). Additionally, we evaluated the protective effect of pyridoxamine (PM), a vitamin B6 analog with anti-AGE effects, in the context of diet-related renal endothelial dysfunction.Methodology: In Wistar rats, the MetS animal model was induced by 20 or 28 weeks of HFD feeding. When indicated, a subgroup of animals was treated daily with PM (60 mg/kg) for 2 months. Tissue perfusion in renal microcirculation was examined by laser speckle contrast imaging. Oxidative stress was analyzed by thiobarbituric acid reactive species and the inflammatory markers by ELISA (TNF-α and IL-1β). Reverse transcription polymerase chain reaction was used to analyze eNOs, IL-6, vascular cell adhesion molecule (VCAM), NADPH oxidase subunit 47 (N47), catalase, and receptor for AGE (RAGE) gene expression.Results: Wistar rats fed a HFD showed negligible alteration in renal function, decrease in catalase mRNA transcripts and catalase enzyme activity compared to control (CTL) animals. Increased levels of IL-1β were observed in the kidney of MetS-induced rats. HFD-fed rats exhibited kidney endothelial dysfunction, with no significant differences in basal microvascular blood flow. PM significantly improved kidney vasorelaxation in HFD-fed rats. eNOS, VCAM, and RAGE gene expression and AGE content were not altered in kidneys of HFD-induced MetS rats in comparison to CTLs.Conclusions: Our findings suggest that HFD-induced microvascular dysfunction precedes the decline in renal function, and could be related to antioxidant machinery defects and inflammation activation in the kidney. PM showed a vasoprotective effect, and thus, could be an important contributory factor in ameliorating diet-induced renal damage.Keywords: metabolic syndrome, kidney endothelial dysfunction, pyridoxamine, advanced glycation end productsRangel Silvares RNunes Goulart da Silva Pereira EEduardo Ilaquita Flores ELino Rodrigues KRibeiro Silva AGonçalves-de-Albuquerque CFDaliry ADove Medical PressarticleMetabolic syndromekidney endothelial dysfunctionpyridoxamineadvanced glycation end products.Specialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 1773-1781 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Metabolic syndrome kidney endothelial dysfunction pyridoxamine advanced glycation end products. Specialties of internal medicine RC581-951
spellingShingle	Metabolic syndrome kidney endothelial dysfunction pyridoxamine advanced glycation end products. Specialties of internal medicine RC581-951 Rangel Silvares R Nunes Goulart da Silva Pereira E Eduardo Ilaquita Flores E Lino Rodrigues K Ribeiro Silva A Gonçalves-de-Albuquerque CF Daliry A High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense
description	Raquel Rangel Silvares1,, Evelyn Nunes Goulart da Silva Pereira1,, Edgar Eduardo Ilaquita Flores1, Karine Lino Rodrigues1, Adriana Ribeiro Silva2, Cassiano Felipe Gonçalves-de-Albuquerque2,3, Anissa Daliry1 1Laboratory of Cardiovascular Investigation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil; 2Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil; 3Laboratory of Immunopharmacology, Federal University of the State of Rio de Janeiro, Rio de Janeiro, RJ, Brazil*These authors contributed equally to this workCorrespondence: Anissa DaliryInstituto Oswaldo Cruz, Fiocruz, Pavilhão Ozório de Almeida, Manguinhos, Rio de Janeiro, CEP: 21.040-360, RJ, BrazilEmail daliry@ioc.fiocruz.brIntroduction: This study aimed to investigate changes in renal function and the AGE-RAGE axis in the kidney of a non-genetic animal model of metabolic syndrome (MetS) induced by high-fat diet (HFD). Additionally, we evaluated the protective effect of pyridoxamine (PM), a vitamin B6 analog with anti-AGE effects, in the context of diet-related renal endothelial dysfunction.Methodology: In Wistar rats, the MetS animal model was induced by 20 or 28 weeks of HFD feeding. When indicated, a subgroup of animals was treated daily with PM (60 mg/kg) for 2 months. Tissue perfusion in renal microcirculation was examined by laser speckle contrast imaging. Oxidative stress was analyzed by thiobarbituric acid reactive species and the inflammatory markers by ELISA (TNF-α and IL-1β). Reverse transcription polymerase chain reaction was used to analyze eNOs, IL-6, vascular cell adhesion molecule (VCAM), NADPH oxidase subunit 47 (N47), catalase, and receptor for AGE (RAGE) gene expression.Results: Wistar rats fed a HFD showed negligible alteration in renal function, decrease in catalase mRNA transcripts and catalase enzyme activity compared to control (CTL) animals. Increased levels of IL-1β were observed in the kidney of MetS-induced rats. HFD-fed rats exhibited kidney endothelial dysfunction, with no significant differences in basal microvascular blood flow. PM significantly improved kidney vasorelaxation in HFD-fed rats. eNOS, VCAM, and RAGE gene expression and AGE content were not altered in kidneys of HFD-induced MetS rats in comparison to CTLs.Conclusions: Our findings suggest that HFD-induced microvascular dysfunction precedes the decline in renal function, and could be related to antioxidant machinery defects and inflammation activation in the kidney. PM showed a vasoprotective effect, and thus, could be an important contributory factor in ameliorating diet-induced renal damage.Keywords: metabolic syndrome, kidney endothelial dysfunction, pyridoxamine, advanced glycation end products
format	article
author	Rangel Silvares R Nunes Goulart da Silva Pereira E Eduardo Ilaquita Flores E Lino Rodrigues K Ribeiro Silva A Gonçalves-de-Albuquerque CF Daliry A
author_facet	Rangel Silvares R Nunes Goulart da Silva Pereira E Eduardo Ilaquita Flores E Lino Rodrigues K Ribeiro Silva A Gonçalves-de-Albuquerque CF Daliry A
author_sort	Rangel Silvares R
title	High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense
title_short	High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense
title_full	High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense
title_fullStr	High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense
title_full_unstemmed	High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense
title_sort	high-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/605b442423bd452da7f8a6a54ce3800f
work_keys_str_mv	AT rangelsilvaresr highfatdietinducedkidneyalterationsinratswithmetabolicsyndromeendothelialdysfunctionanddecreasedantioxidantdefense AT nunesgoulartdasilvapereirae highfatdietinducedkidneyalterationsinratswithmetabolicsyndromeendothelialdysfunctionanddecreasedantioxidantdefense AT eduardoilaquitaflorese highfatdietinducedkidneyalterationsinratswithmetabolicsyndromeendothelialdysfunctionanddecreasedantioxidantdefense AT linorodriguesk highfatdietinducedkidneyalterationsinratswithmetabolicsyndromeendothelialdysfunctionanddecreasedantioxidantdefense AT ribeirosilvaa highfatdietinducedkidneyalterationsinratswithmetabolicsyndromeendothelialdysfunctionanddecreasedantioxidantdefense AT goncalvesdealbuquerquecf highfatdietinducedkidneyalterationsinratswithmetabolicsyndromeendothelialdysfunctionanddecreasedantioxidantdefense AT dalirya highfatdietinducedkidneyalterationsinratswithmetabolicsyndromeendothelialdysfunctionanddecreasedantioxidantdefense
_version_	1718398247273234432

High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense

Ejemplares similares