Production, characterization and applications for Toxoplasma gondii-specific polyclonal chicken egg yolk immunoglobulins.

<h4>Background</h4>Toxoplasma gondii may cause abortions, ocular and neurological disorders in warm-blood hosts. Immunized mammals are a wide source of hyperimmune sera used in different approaches, including diagnosis and the study of host-parasite interactions. Unfortunately, mammalian...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Álvaro Ferreira Júnior, Fernanda M Santiago, Murilo V Silva, Flávia B Ferreira, Arlindo G Macêdo Júnior, Caroline M Mota, Matheus S Faria, Hercílio H Silva Filho, Deise A O Silva, Jair P Cunha-Júnior, José R Mineo, Tiago W P Mineo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/605e4072dc304b2ca47a4778cd583daf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:605e4072dc304b2ca47a4778cd583daf
record_format	dspace
spelling	oai:doaj.org-article:605e4072dc304b2ca47a4778cd583daf2021-11-18T07:12:41ZProduction, characterization and applications for Toxoplasma gondii-specific polyclonal chicken egg yolk immunoglobulins.1932-620310.1371/journal.pone.0040391https://doaj.org/article/605e4072dc304b2ca47a4778cd583daf2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22808150/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Toxoplasma gondii may cause abortions, ocular and neurological disorders in warm-blood hosts. Immunized mammals are a wide source of hyperimmune sera used in different approaches, including diagnosis and the study of host-parasite interactions. Unfortunately, mammalian antibodies present limitations for its production, such as the necessity for animal bleeding, low yield, interference with rheumatoid factor, complement activation and affinity to Fc mammalian receptors. IgY antibodies avoid those limitations; therefore they could be an alternative to be applied in T. gondii model.<h4>Methodology/principal findings</h4>In this study we immunized hens with soluble tachyzoite antigens of T. gondii (STAg) and purified egg yolk antibodies (IgY) by an inexpensive and simple method, with high yield and purity degree. IgY anti-STAg antibodies presented high avidity and were able to recognize a broad range of parasite antigens, although some marked differences were observed in reactivity profile between antibodies produced in immunized hens and mice. Interestingly, IgY antibodies against Neospora caninum and Eimeria spp. did not react to STAg. We also show that IgY antibodies were suitable to detect T. gondii forms in paraffin-embedded sections and culture cell monolayers.<h4>Conclusions/significance</h4>Due to its cost-effectiveness, high production yield and varied range of possible applications, polyclonal IgY antibodies are useful tools for studies involving T. gondii.Álvaro Ferreira JúniorFernanda M SantiagoMurilo V SilvaFlávia B FerreiraArlindo G Macêdo JúniorCaroline M MotaMatheus S FariaHercílio H Silva FilhoDeise A O SilvaJair P Cunha-JúniorJosé R MineoTiago W P MineoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40391 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Álvaro Ferreira Júnior Fernanda M Santiago Murilo V Silva Flávia B Ferreira Arlindo G Macêdo Júnior Caroline M Mota Matheus S Faria Hercílio H Silva Filho Deise A O Silva Jair P Cunha-Júnior José R Mineo Tiago W P Mineo Production, characterization and applications for Toxoplasma gondii-specific polyclonal chicken egg yolk immunoglobulins.
description	<h4>Background</h4>Toxoplasma gondii may cause abortions, ocular and neurological disorders in warm-blood hosts. Immunized mammals are a wide source of hyperimmune sera used in different approaches, including diagnosis and the study of host-parasite interactions. Unfortunately, mammalian antibodies present limitations for its production, such as the necessity for animal bleeding, low yield, interference with rheumatoid factor, complement activation and affinity to Fc mammalian receptors. IgY antibodies avoid those limitations; therefore they could be an alternative to be applied in T. gondii model.<h4>Methodology/principal findings</h4>In this study we immunized hens with soluble tachyzoite antigens of T. gondii (STAg) and purified egg yolk antibodies (IgY) by an inexpensive and simple method, with high yield and purity degree. IgY anti-STAg antibodies presented high avidity and were able to recognize a broad range of parasite antigens, although some marked differences were observed in reactivity profile between antibodies produced in immunized hens and mice. Interestingly, IgY antibodies against Neospora caninum and Eimeria spp. did not react to STAg. We also show that IgY antibodies were suitable to detect T. gondii forms in paraffin-embedded sections and culture cell monolayers.<h4>Conclusions/significance</h4>Due to its cost-effectiveness, high production yield and varied range of possible applications, polyclonal IgY antibodies are useful tools for studies involving T. gondii.
format	article
author	Álvaro Ferreira Júnior Fernanda M Santiago Murilo V Silva Flávia B Ferreira Arlindo G Macêdo Júnior Caroline M Mota Matheus S Faria Hercílio H Silva Filho Deise A O Silva Jair P Cunha-Júnior José R Mineo Tiago W P Mineo
author_facet	Álvaro Ferreira Júnior Fernanda M Santiago Murilo V Silva Flávia B Ferreira Arlindo G Macêdo Júnior Caroline M Mota Matheus S Faria Hercílio H Silva Filho Deise A O Silva Jair P Cunha-Júnior José R Mineo Tiago W P Mineo
author_sort	Álvaro Ferreira Júnior
title	Production, characterization and applications for Toxoplasma gondii-specific polyclonal chicken egg yolk immunoglobulins.
title_short	Production, characterization and applications for Toxoplasma gondii-specific polyclonal chicken egg yolk immunoglobulins.
title_full	Production, characterization and applications for Toxoplasma gondii-specific polyclonal chicken egg yolk immunoglobulins.
title_fullStr	Production, characterization and applications for Toxoplasma gondii-specific polyclonal chicken egg yolk immunoglobulins.
title_full_unstemmed	Production, characterization and applications for Toxoplasma gondii-specific polyclonal chicken egg yolk immunoglobulins.
title_sort	production, characterization and applications for toxoplasma gondii-specific polyclonal chicken egg yolk immunoglobulins.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/605e4072dc304b2ca47a4778cd583daf
work_keys_str_mv	AT alvaroferreirajunior productioncharacterizationandapplicationsfortoxoplasmagondiispecificpolyclonalchickeneggyolkimmunoglobulins AT fernandamsantiago productioncharacterizationandapplicationsfortoxoplasmagondiispecificpolyclonalchickeneggyolkimmunoglobulins AT murilovsilva productioncharacterizationandapplicationsfortoxoplasmagondiispecificpolyclonalchickeneggyolkimmunoglobulins AT flaviabferreira productioncharacterizationandapplicationsfortoxoplasmagondiispecificpolyclonalchickeneggyolkimmunoglobulins AT arlindogmacedojunior productioncharacterizationandapplicationsfortoxoplasmagondiispecificpolyclonalchickeneggyolkimmunoglobulins AT carolinemmota productioncharacterizationandapplicationsfortoxoplasmagondiispecificpolyclonalchickeneggyolkimmunoglobulins AT matheussfaria productioncharacterizationandapplicationsfortoxoplasmagondiispecificpolyclonalchickeneggyolkimmunoglobulins AT herciliohsilvafilho productioncharacterizationandapplicationsfortoxoplasmagondiispecificpolyclonalchickeneggyolkimmunoglobulins AT deiseaosilva productioncharacterizationandapplicationsfortoxoplasmagondiispecificpolyclonalchickeneggyolkimmunoglobulins AT jairpcunhajunior productioncharacterizationandapplicationsfortoxoplasmagondiispecificpolyclonalchickeneggyolkimmunoglobulins AT josermineo productioncharacterizationandapplicationsfortoxoplasmagondiispecificpolyclonalchickeneggyolkimmunoglobulins AT tiagowpmineo productioncharacterizationandapplicationsfortoxoplasmagondiispecificpolyclonalchickeneggyolkimmunoglobulins
_version_	1718423789835911168

Production, characterization and applications for Toxoplasma gondii-specific polyclonal chicken egg yolk immunoglobulins.

Ejemplares similares