An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo

The authors have previously reported split-GFP-based contact site sensors (SPLICS) to document endoplasmic reticulum/mitochondria contact sites. Here they extend this work and develop a range of improved SPLICS sensors to detect single and multiple organelle contact sites at different distances.

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Autores principales: Francesca Vallese, Cristina Catoni, Domenico Cieri, Lucia Barazzuol, Omar Ramirez, Valentina Calore, Massimo Bonora, Flavia Giamogante, Paolo Pinton, Marisa Brini, Tito Calì
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Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/605e86eacf834bb7bdf8a03fe68ceb5e
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spelling oai:doaj.org-article:605e86eacf834bb7bdf8a03fe68ceb5e2021-12-02T17:31:25ZAn expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo10.1038/s41467-020-19892-62041-1723https://doaj.org/article/605e86eacf834bb7bdf8a03fe68ceb5e2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19892-6https://doaj.org/toc/2041-1723The authors have previously reported split-GFP-based contact site sensors (SPLICS) to document endoplasmic reticulum/mitochondria contact sites. Here they extend this work and develop a range of improved SPLICS sensors to detect single and multiple organelle contact sites at different distances.Francesca ValleseCristina CatoniDomenico CieriLucia BarazzuolOmar RamirezValentina CaloreMassimo BonoraFlavia GiamogantePaolo PintonMarisa BriniTito CalìNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Francesca Vallese
Cristina Catoni
Domenico Cieri
Lucia Barazzuol
Omar Ramirez
Valentina Calore
Massimo Bonora
Flavia Giamogante
Paolo Pinton
Marisa Brini
Tito Calì
An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo
description The authors have previously reported split-GFP-based contact site sensors (SPLICS) to document endoplasmic reticulum/mitochondria contact sites. Here they extend this work and develop a range of improved SPLICS sensors to detect single and multiple organelle contact sites at different distances.
format article
author Francesca Vallese
Cristina Catoni
Domenico Cieri
Lucia Barazzuol
Omar Ramirez
Valentina Calore
Massimo Bonora
Flavia Giamogante
Paolo Pinton
Marisa Brini
Tito Calì
author_facet Francesca Vallese
Cristina Catoni
Domenico Cieri
Lucia Barazzuol
Omar Ramirez
Valentina Calore
Massimo Bonora
Flavia Giamogante
Paolo Pinton
Marisa Brini
Tito Calì
author_sort Francesca Vallese
title An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo
title_short An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo
title_full An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo
title_fullStr An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo
title_full_unstemmed An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo
title_sort expanded palette of improved splics reporters detects multiple organelle contacts in vitro and in vivo
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/605e86eacf834bb7bdf8a03fe68ceb5e
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