Electrolysis of selectively patterned Vorticella with pneumatic microchambers and electrodes

Living machines are expected to expand the limits of silicon microtechnology. Vorticella convallaria is a ciliated protozoa and a promising linear actuator. The use of Vorticella as a Ca2+-responsive actuator, in a small system, requires a reduction in the size of the membrane treatment of selective...

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Autores principales:	Moeto NAGAI, Ryoga KOHARA, Tuhin Subhra SANTRA, Takayuki SHIBATA
Formato:	article
Lenguaje:	EN
Publicado:	The Japan Society of Mechanical Engineers 2020
Materias:	selective patterning electrochemical treatment bioactuator pneumatic channel vorticella permeabilization Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/606ca3891cb543f490708d1403be1b48
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spelling	oai:doaj.org-article:606ca3891cb543f490708d1403be1b482021-11-29T06:04:29ZElectrolysis of selectively patterned Vorticella with pneumatic microchambers and electrodes2187-974510.1299/mej.20-00254https://doaj.org/article/606ca3891cb543f490708d1403be1b482020-11-01T00:00:00Zhttps://www.jstage.jst.go.jp/article/mej/8/1/8_20-00254/_pdf/-char/enhttps://doaj.org/toc/2187-9745Living machines are expected to expand the limits of silicon microtechnology. Vorticella convallaria is a ciliated protozoa and a promising linear actuator. The use of Vorticella as a Ca2+-responsive actuator, in a small system, requires a reduction in the size of the membrane treatment of selectively patterned Vorticella. However, selective cell patterning and the electrolysis of cell membranes have not yet been combined; therefore, the development of a combined method is essential. The objective of this study is to develop a method for combining selective cell patterning and the electrolysis of cell membranes. A two-layer pneumatic device was fabricated in polydimethylsiloxane (PDMS), and the device and electrodes were clamped with acrylic plates. The deformation of the PDMS membrane was controlled by compressed air. The electrochemical reactions were visualized with fluorescein, and the temporal pH change was evaluated. Vorticella cells were introduced into a microfluidic device and were captured in chambers at a pressure of 21 kPa. They were immobilized in the chambers after incubation for 6 h. Further, OH– was generated from the cathode by the electrolysis of water, and the bell-shaped bodies of Vorticella became spherical. This permeabilization treatment stopped both spontaneous contraction and cilia movement. The application of DC 3 V was sufficient for the electrolysis of Vorticella. Selective patterning and electrochemical membrane treatments are beneficial for facilitating the use of Vorticella as a Ca2+ responsive actuator in a small system.Moeto NAGAIRyoga KOHARATuhin Subhra SANTRATakayuki SHIBATAThe Japan Society of Mechanical Engineersarticleselective patterningelectrochemical treatmentbioactuatorpneumatic channelvorticellapermeabilizationMechanical engineering and machineryTJ1-1570ENMechanical Engineering Journal, Vol 8, Iss 1, Pp 20-00254-20-00254 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	selective patterning electrochemical treatment bioactuator pneumatic channel vorticella permeabilization Mechanical engineering and machinery TJ1-1570
spellingShingle	selective patterning electrochemical treatment bioactuator pneumatic channel vorticella permeabilization Mechanical engineering and machinery TJ1-1570 Moeto NAGAI Ryoga KOHARA Tuhin Subhra SANTRA Takayuki SHIBATA Electrolysis of selectively patterned Vorticella with pneumatic microchambers and electrodes
description	Living machines are expected to expand the limits of silicon microtechnology. Vorticella convallaria is a ciliated protozoa and a promising linear actuator. The use of Vorticella as a Ca2+-responsive actuator, in a small system, requires a reduction in the size of the membrane treatment of selectively patterned Vorticella. However, selective cell patterning and the electrolysis of cell membranes have not yet been combined; therefore, the development of a combined method is essential. The objective of this study is to develop a method for combining selective cell patterning and the electrolysis of cell membranes. A two-layer pneumatic device was fabricated in polydimethylsiloxane (PDMS), and the device and electrodes were clamped with acrylic plates. The deformation of the PDMS membrane was controlled by compressed air. The electrochemical reactions were visualized with fluorescein, and the temporal pH change was evaluated. Vorticella cells were introduced into a microfluidic device and were captured in chambers at a pressure of 21 kPa. They were immobilized in the chambers after incubation for 6 h. Further, OH– was generated from the cathode by the electrolysis of water, and the bell-shaped bodies of Vorticella became spherical. This permeabilization treatment stopped both spontaneous contraction and cilia movement. The application of DC 3 V was sufficient for the electrolysis of Vorticella. Selective patterning and electrochemical membrane treatments are beneficial for facilitating the use of Vorticella as a Ca2+ responsive actuator in a small system.
format	article
author	Moeto NAGAI Ryoga KOHARA Tuhin Subhra SANTRA Takayuki SHIBATA
author_facet	Moeto NAGAI Ryoga KOHARA Tuhin Subhra SANTRA Takayuki SHIBATA
author_sort	Moeto NAGAI
title	Electrolysis of selectively patterned Vorticella with pneumatic microchambers and electrodes
title_short	Electrolysis of selectively patterned Vorticella with pneumatic microchambers and electrodes
title_full	Electrolysis of selectively patterned Vorticella with pneumatic microchambers and electrodes
title_fullStr	Electrolysis of selectively patterned Vorticella with pneumatic microchambers and electrodes
title_full_unstemmed	Electrolysis of selectively patterned Vorticella with pneumatic microchambers and electrodes
title_sort	electrolysis of selectively patterned vorticella with pneumatic microchambers and electrodes
publisher	The Japan Society of Mechanical Engineers
publishDate	2020
url	https://doaj.org/article/606ca3891cb543f490708d1403be1b48
work_keys_str_mv	AT moetonagai electrolysisofselectivelypatternedvorticellawithpneumaticmicrochambersandelectrodes AT ryogakohara electrolysisofselectivelypatternedvorticellawithpneumaticmicrochambersandelectrodes AT tuhinsubhrasantra electrolysisofselectivelypatternedvorticellawithpneumaticmicrochambersandelectrodes AT takayukishibata electrolysisofselectivelypatternedvorticellawithpneumaticmicrochambersandelectrodes
_version_	1718407587319250944

Electrolysis of selectively patterned Vorticella with pneumatic microchambers and electrodes

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