Effects of Mesenchymal Stem Cell-Derived Exosomes on Experimental Autoimmune Uveitis

Abstract We previously demonstrated that mesenchymal stem cells (MSCs) ameliorated experimental autoimmune uveoretinitis (EAU) in rats. Recently, MSC-derived exosomes (MSC-Exo) were thought to carry functions of MSCs. In this study, we tested the effect of local administration of human MSC-Exo on es...

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Autores principales:	Lingling Bai, Hui Shao, Hongxing Wang, Zhihui Zhang, Chang Su, Lijie Dong, Bo Yu, Xiteng Chen, Xiaorong Li, Xiaomin Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/606d1ee47ce44c4ca65ad0ff70d26885
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spelling	oai:doaj.org-article:606d1ee47ce44c4ca65ad0ff70d268852021-12-02T11:41:00ZEffects of Mesenchymal Stem Cell-Derived Exosomes on Experimental Autoimmune Uveitis10.1038/s41598-017-04559-y2045-2322https://doaj.org/article/606d1ee47ce44c4ca65ad0ff70d268852017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04559-yhttps://doaj.org/toc/2045-2322Abstract We previously demonstrated that mesenchymal stem cells (MSCs) ameliorated experimental autoimmune uveoretinitis (EAU) in rats. Recently, MSC-derived exosomes (MSC-Exo) were thought to carry functions of MSCs. In this study, we tested the effect of local administration of human MSC-Exo on established EAU in the same species. Rats with EAU induced by immunization with interphotoreceptor retinol-binding protein 1177–1191 peptide were treated by periocular injections of increasing doses of MSC-Exo starting at the disease onset for 7 consecutive days. The in vitro effects of MSC-Exo on immune cell migration and responder T cell proliferation were examined by chemotactic assays and lymphocyte proliferation assays, respectively. We found that MSC-Exo greatly reduced the intensity of ongoing EAU as their parent cells by reducing the infiltration of T cell subsets, and other inflammatory cells, in the eyes. Furthermore, the chemoattractive effects of CCL2 and CCL21 on inflammatory cells were inhibited by MSC-Exo. However, no inhibitory effect of MSC-Exo on IRBP-specific T cell proliferation was observed. These results suggest that MSC-Exo effectively ameliorate EAU by inhibiting the migration of inflammatory cells, indicating a potential novel therapy of MSC-Exo for uveitis.Lingling BaiHui ShaoHongxing WangZhihui ZhangChang SuLijie DongBo YuXiteng ChenXiaorong LiXiaomin ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lingling Bai Hui Shao Hongxing Wang Zhihui Zhang Chang Su Lijie Dong Bo Yu Xiteng Chen Xiaorong Li Xiaomin Zhang Effects of Mesenchymal Stem Cell-Derived Exosomes on Experimental Autoimmune Uveitis
description	Abstract We previously demonstrated that mesenchymal stem cells (MSCs) ameliorated experimental autoimmune uveoretinitis (EAU) in rats. Recently, MSC-derived exosomes (MSC-Exo) were thought to carry functions of MSCs. In this study, we tested the effect of local administration of human MSC-Exo on established EAU in the same species. Rats with EAU induced by immunization with interphotoreceptor retinol-binding protein 1177–1191 peptide were treated by periocular injections of increasing doses of MSC-Exo starting at the disease onset for 7 consecutive days. The in vitro effects of MSC-Exo on immune cell migration and responder T cell proliferation were examined by chemotactic assays and lymphocyte proliferation assays, respectively. We found that MSC-Exo greatly reduced the intensity of ongoing EAU as their parent cells by reducing the infiltration of T cell subsets, and other inflammatory cells, in the eyes. Furthermore, the chemoattractive effects of CCL2 and CCL21 on inflammatory cells were inhibited by MSC-Exo. However, no inhibitory effect of MSC-Exo on IRBP-specific T cell proliferation was observed. These results suggest that MSC-Exo effectively ameliorate EAU by inhibiting the migration of inflammatory cells, indicating a potential novel therapy of MSC-Exo for uveitis.
format	article
author	Lingling Bai Hui Shao Hongxing Wang Zhihui Zhang Chang Su Lijie Dong Bo Yu Xiteng Chen Xiaorong Li Xiaomin Zhang
author_facet	Lingling Bai Hui Shao Hongxing Wang Zhihui Zhang Chang Su Lijie Dong Bo Yu Xiteng Chen Xiaorong Li Xiaomin Zhang
author_sort	Lingling Bai
title	Effects of Mesenchymal Stem Cell-Derived Exosomes on Experimental Autoimmune Uveitis
title_short	Effects of Mesenchymal Stem Cell-Derived Exosomes on Experimental Autoimmune Uveitis
title_full	Effects of Mesenchymal Stem Cell-Derived Exosomes on Experimental Autoimmune Uveitis
title_fullStr	Effects of Mesenchymal Stem Cell-Derived Exosomes on Experimental Autoimmune Uveitis
title_full_unstemmed	Effects of Mesenchymal Stem Cell-Derived Exosomes on Experimental Autoimmune Uveitis
title_sort	effects of mesenchymal stem cell-derived exosomes on experimental autoimmune uveitis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/606d1ee47ce44c4ca65ad0ff70d26885
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Effects of Mesenchymal Stem Cell-Derived Exosomes on Experimental Autoimmune Uveitis

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