NEPHRITOGENIC ACTIVITY OF IgA-BINDING STREPTOCOCCUS PYOGENES: AN EXPERIMENTAL MODEL OF IgA GLOMERULONEPHRITIS

The aim of present study was to arrange an experimental rabbit model for IgA-nephropathy. To this purpose, an approach was attempted which was previously successfully applied in rabbits, aiming for induction of post-streptococcal glomerulonephritis (PSGN). To induce the nephropathy, we used two Stre...

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Autores principales:	L. A. Burova, P. V. Pigarevsky, V. A. Snegova, N. V. Duplik, Klaas Schalen, Artem A. Totolian
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2016
Materias:	streptococcus pyogenes iga-binding streptococci iga nephropathy experimental model Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/60712910468746d182d38937dcb70f5a
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spelling	oai:doaj.org-article:60712910468746d182d38937dcb70f5a2021-11-18T08:03:45ZNEPHRITOGENIC ACTIVITY OF IgA-BINDING STREPTOCOCCUS PYOGENES: AN EXPERIMENTAL MODEL OF IgA GLOMERULONEPHRITIS1563-06252313-741X10.15789/1563-0625-2016-3-221-230https://doaj.org/article/60712910468746d182d38937dcb70f5a2016-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1034https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe aim of present study was to arrange an experimental rabbit model for IgA-nephropathy. To this purpose, an approach was attempted which was previously successfully applied in rabbits, aiming for induction of post-streptococcal glomerulonephritis (PSGN). To induce the nephropathy, we used two Streptococcus pyogenes strains of M4 and M60 serotypes which showed differential IgA-binding capacity mediated by the IgAFc microbial receptors. The renal tissue damage was developed in most animals treated with emm60 S. pyogenes characterized by marked IgAFcR expression and higher IgA-binding ability. By means of morphometric analysis. Significant morphological and immunochemical glomerular changes were revealed in 6/10 rabbits, as follows: (i) massive IgA deposition in the mesangial glomerular cells, atrophy of the capillary net, and tissue oedema; (ii) marked C3-complement deposition in proximal and distal tubules; (iii) a significant infiltration of cortical and medullar areas by lymphocytes associated with weak TNFα production. Noteworthy, we did not observe local IgG deposition in any cases, thus allowing to exclude any role of anti-IgG, or other IgG’s in evolution of the pathology. Alternatively, the IgA-deposits may occur due to microbial IgA FcR–IgAcontaining cоmplexes, as earlier shown by the Swedish scientists. The above tissue changes were completely absent in kidneys of control animals. Taken together, these data suggest that we have developed an experimental model similar to IgA-nephropathy in humans. The results also extend our knowledge on pathogenic effects of the IgA Fc-binding proteins of Streptococcus pyogenes.L. A. BurovaP. V. PigarevskyV. A. SnegovaN. V. DuplikKlaas SchalenArtem A. TotolianSPb RAACIarticlestreptococcus pyogenesiga-binding streptococciiga nephropathyexperimental modelImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 18, Iss 3, Pp 221-230 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	streptococcus pyogenes iga-binding streptococci iga nephropathy experimental model Immunologic diseases. Allergy RC581-607
spellingShingle	streptococcus pyogenes iga-binding streptococci iga nephropathy experimental model Immunologic diseases. Allergy RC581-607 L. A. Burova P. V. Pigarevsky V. A. Snegova N. V. Duplik Klaas Schalen Artem A. Totolian NEPHRITOGENIC ACTIVITY OF IgA-BINDING STREPTOCOCCUS PYOGENES: AN EXPERIMENTAL MODEL OF IgA GLOMERULONEPHRITIS
description	The aim of present study was to arrange an experimental rabbit model for IgA-nephropathy. To this purpose, an approach was attempted which was previously successfully applied in rabbits, aiming for induction of post-streptococcal glomerulonephritis (PSGN). To induce the nephropathy, we used two Streptococcus pyogenes strains of M4 and M60 serotypes which showed differential IgA-binding capacity mediated by the IgAFc microbial receptors. The renal tissue damage was developed in most animals treated with emm60 S. pyogenes characterized by marked IgAFcR expression and higher IgA-binding ability. By means of morphometric analysis. Significant morphological and immunochemical glomerular changes were revealed in 6/10 rabbits, as follows: (i) massive IgA deposition in the mesangial glomerular cells, atrophy of the capillary net, and tissue oedema; (ii) marked C3-complement deposition in proximal and distal tubules; (iii) a significant infiltration of cortical and medullar areas by lymphocytes associated with weak TNFα production. Noteworthy, we did not observe local IgG deposition in any cases, thus allowing to exclude any role of anti-IgG, or other IgG’s in evolution of the pathology. Alternatively, the IgA-deposits may occur due to microbial IgA FcR–IgAcontaining cоmplexes, as earlier shown by the Swedish scientists. The above tissue changes were completely absent in kidneys of control animals. Taken together, these data suggest that we have developed an experimental model similar to IgA-nephropathy in humans. The results also extend our knowledge on pathogenic effects of the IgA Fc-binding proteins of Streptococcus pyogenes.
format	article
author	L. A. Burova P. V. Pigarevsky V. A. Snegova N. V. Duplik Klaas Schalen Artem A. Totolian
author_facet	L. A. Burova P. V. Pigarevsky V. A. Snegova N. V. Duplik Klaas Schalen Artem A. Totolian
author_sort	L. A. Burova
title	NEPHRITOGENIC ACTIVITY OF IgA-BINDING STREPTOCOCCUS PYOGENES: AN EXPERIMENTAL MODEL OF IgA GLOMERULONEPHRITIS
title_short	NEPHRITOGENIC ACTIVITY OF IgA-BINDING STREPTOCOCCUS PYOGENES: AN EXPERIMENTAL MODEL OF IgA GLOMERULONEPHRITIS
title_full	NEPHRITOGENIC ACTIVITY OF IgA-BINDING STREPTOCOCCUS PYOGENES: AN EXPERIMENTAL MODEL OF IgA GLOMERULONEPHRITIS
title_fullStr	NEPHRITOGENIC ACTIVITY OF IgA-BINDING STREPTOCOCCUS PYOGENES: AN EXPERIMENTAL MODEL OF IgA GLOMERULONEPHRITIS
title_full_unstemmed	NEPHRITOGENIC ACTIVITY OF IgA-BINDING STREPTOCOCCUS PYOGENES: AN EXPERIMENTAL MODEL OF IgA GLOMERULONEPHRITIS
title_sort	nephritogenic activity of iga-binding streptococcus pyogenes: an experimental model of iga glomerulonephritis
publisher	SPb RAACI
publishDate	2016
url	https://doaj.org/article/60712910468746d182d38937dcb70f5a
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NEPHRITOGENIC ACTIVITY OF IgA-BINDING STREPTOCOCCUS PYOGENES: AN EXPERIMENTAL MODEL OF IgA GLOMERULONEPHRITIS

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