Identification of SNP rs1799854 ABCC8 gene and blood glucose levels in patients with type 2 diabetes mellitus at Moewardi hospital Surakarta Solo

Type 2 diabetes mellitus (T2DM) is a complex metabolic disease characterized by high blood glucose levels due to impaired insulin secretion or insulin resistance. Polymorphisms in the ABCC8 gene rs1799854 are widely found to have an association with T2DM, where the ABCC8 gene encodes the SUR1 protei...

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Autores principales:	Mardiana Puji Lestari, Imaniar Noor Faridah, Rita Maliza, Haafizah Dania, Melinda Widianingrum, Dyah Aryani Perwitasari
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Ahmad Dahlan 2021
Materias:	t2dm sulfonylurea abcc8 gene outcome therapy snp Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/60742d07bf9c436c8d7b1024e498a824
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spelling	oai:doaj.org-article:60742d07bf9c436c8d7b1024e498a8242021-11-17T06:28:43Z Identification of SNP rs1799854 ABCC8 gene and blood glucose levels in patients with type 2 diabetes mellitus at Moewardi hospital Surakarta Solo10.12928/pharmaciana.v11i3.191002088-45592477-0256https://doaj.org/article/60742d07bf9c436c8d7b1024e498a8242021-11-01T00:00:00Zhttp://journal.uad.ac.id/index.php/PHARMACIANA/article/view/19100/pdf_206https://doaj.org/toc/2088-4559https://doaj.org/toc/2477-0256Type 2 diabetes mellitus (T2DM) is a complex metabolic disease characterized by high blood glucose levels due to impaired insulin secretion or insulin resistance. Polymorphisms in the ABCC8 gene rs1799854 are widely found to have an association with T2DM, where the ABCC8 gene encodes the SUR1 protein from the K-ATP channel that plays a role in insulin secretion in cells β pancreas. Mutations in the ABCC8 gene can cause potassium channels and insulin secretions problems that possibly decrease the effectiveness of the drug. Polymorphisms in some populations have been reported, but similar research on Solo's population has never been conducted. The purpose of this study is to identify the genotype of the ABCC8 gene in rs1799854 and the therapeutic outcome of blood glucose levels in T2DM patients. This research is cross-sectional research conducted prospectively at Moewardi Hospital Surakarta, Solo. Blood samples were collected from 10 T2DM patients who received sulfonylurea monotherapy taken through veins for genotype examination by DNA isolation, PCR amplification, and sequencing. Parameters of fasting blood glucose (FBG), 2-hour postprandial blood glucose, and HbA1c are measured as therapeutic outcomes. We found the dominant results in a mutant homozygote (TT) 40%; while wild type (CC); and a mutant heterozygote (CT) was 30%. Single Nucleotide Polymorphisms (SNP) of ABCC8 gene rs1799854, found in T2DM patients at Moewardi Hospital Surakarta, Solo, who received sulfonylurea therapy.Mardiana Puji LestariImaniar Noor FaridahRita MalizaHaafizah DaniaMelinda WidianingrumDyah Aryani PerwitasariUniversitas Ahmad Dahlanarticlet2dmsulfonylureaabcc8 geneoutcome therapysnpPharmacy and materia medicaRS1-441ENPharmaciana, Vol 11, Iss 3, Pp 338-346 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	t2dm sulfonylurea abcc8 gene outcome therapy snp Pharmacy and materia medica RS1-441
spellingShingle	t2dm sulfonylurea abcc8 gene outcome therapy snp Pharmacy and materia medica RS1-441 Mardiana Puji Lestari Imaniar Noor Faridah Rita Maliza Haafizah Dania Melinda Widianingrum Dyah Aryani Perwitasari Identification of SNP rs1799854 ABCC8 gene and blood glucose levels in patients with type 2 diabetes mellitus at Moewardi hospital Surakarta Solo
description	Type 2 diabetes mellitus (T2DM) is a complex metabolic disease characterized by high blood glucose levels due to impaired insulin secretion or insulin resistance. Polymorphisms in the ABCC8 gene rs1799854 are widely found to have an association with T2DM, where the ABCC8 gene encodes the SUR1 protein from the K-ATP channel that plays a role in insulin secretion in cells β pancreas. Mutations in the ABCC8 gene can cause potassium channels and insulin secretions problems that possibly decrease the effectiveness of the drug. Polymorphisms in some populations have been reported, but similar research on Solo's population has never been conducted. The purpose of this study is to identify the genotype of the ABCC8 gene in rs1799854 and the therapeutic outcome of blood glucose levels in T2DM patients. This research is cross-sectional research conducted prospectively at Moewardi Hospital Surakarta, Solo. Blood samples were collected from 10 T2DM patients who received sulfonylurea monotherapy taken through veins for genotype examination by DNA isolation, PCR amplification, and sequencing. Parameters of fasting blood glucose (FBG), 2-hour postprandial blood glucose, and HbA1c are measured as therapeutic outcomes. We found the dominant results in a mutant homozygote (TT) 40%; while wild type (CC); and a mutant heterozygote (CT) was 30%. Single Nucleotide Polymorphisms (SNP) of ABCC8 gene rs1799854, found in T2DM patients at Moewardi Hospital Surakarta, Solo, who received sulfonylurea therapy.
format	article
author	Mardiana Puji Lestari Imaniar Noor Faridah Rita Maliza Haafizah Dania Melinda Widianingrum Dyah Aryani Perwitasari
author_facet	Mardiana Puji Lestari Imaniar Noor Faridah Rita Maliza Haafizah Dania Melinda Widianingrum Dyah Aryani Perwitasari
author_sort	Mardiana Puji Lestari
title	Identification of SNP rs1799854 ABCC8 gene and blood glucose levels in patients with type 2 diabetes mellitus at Moewardi hospital Surakarta Solo
title_short	Identification of SNP rs1799854 ABCC8 gene and blood glucose levels in patients with type 2 diabetes mellitus at Moewardi hospital Surakarta Solo
title_full	Identification of SNP rs1799854 ABCC8 gene and blood glucose levels in patients with type 2 diabetes mellitus at Moewardi hospital Surakarta Solo
title_fullStr	Identification of SNP rs1799854 ABCC8 gene and blood glucose levels in patients with type 2 diabetes mellitus at Moewardi hospital Surakarta Solo
title_full_unstemmed	Identification of SNP rs1799854 ABCC8 gene and blood glucose levels in patients with type 2 diabetes mellitus at Moewardi hospital Surakarta Solo
title_sort	identification of snp rs1799854 abcc8 gene and blood glucose levels in patients with type 2 diabetes mellitus at moewardi hospital surakarta solo
publisher	Universitas Ahmad Dahlan
publishDate	2021
url	https://doaj.org/article/60742d07bf9c436c8d7b1024e498a824
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Identification of SNP rs1799854 ABCC8 gene and blood glucose levels in patients with type 2 diabetes mellitus at Moewardi hospital Surakarta Solo

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