Multifocal imaging for precise, label-free tracking of fast biological processes in 3D

Multifocal imaging suffers from a number of limitations. Here the authors report an open-source 3D reconstruction algorithm to enable label-free tracking of spherical and filamentous structures which they use to characterise fluid flow and flagellar beating of human and sea urchin sperm.

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Autores principales: Jan N. Hansen, An Gong, Dagmar Wachten, René Pascal, Alex Turpin, Jan F. Jikeli, U. Benjamin Kaupp, Luis Alvarez
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/6077319b1f614bdbad31c204d6adbb5d
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spelling oai:doaj.org-article:6077319b1f614bdbad31c204d6adbb5d2021-12-02T16:13:56ZMultifocal imaging for precise, label-free tracking of fast biological processes in 3D10.1038/s41467-021-24768-42041-1723https://doaj.org/article/6077319b1f614bdbad31c204d6adbb5d2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24768-4https://doaj.org/toc/2041-1723Multifocal imaging suffers from a number of limitations. Here the authors report an open-source 3D reconstruction algorithm to enable label-free tracking of spherical and filamentous structures which they use to characterise fluid flow and flagellar beating of human and sea urchin sperm.Jan N. HansenAn GongDagmar WachtenRené PascalAlex TurpinJan F. JikeliU. Benjamin KauppLuis AlvarezNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Jan N. Hansen
An Gong
Dagmar Wachten
René Pascal
Alex Turpin
Jan F. Jikeli
U. Benjamin Kaupp
Luis Alvarez
Multifocal imaging for precise, label-free tracking of fast biological processes in 3D
description Multifocal imaging suffers from a number of limitations. Here the authors report an open-source 3D reconstruction algorithm to enable label-free tracking of spherical and filamentous structures which they use to characterise fluid flow and flagellar beating of human and sea urchin sperm.
format article
author Jan N. Hansen
An Gong
Dagmar Wachten
René Pascal
Alex Turpin
Jan F. Jikeli
U. Benjamin Kaupp
Luis Alvarez
author_facet Jan N. Hansen
An Gong
Dagmar Wachten
René Pascal
Alex Turpin
Jan F. Jikeli
U. Benjamin Kaupp
Luis Alvarez
author_sort Jan N. Hansen
title Multifocal imaging for precise, label-free tracking of fast biological processes in 3D
title_short Multifocal imaging for precise, label-free tracking of fast biological processes in 3D
title_full Multifocal imaging for precise, label-free tracking of fast biological processes in 3D
title_fullStr Multifocal imaging for precise, label-free tracking of fast biological processes in 3D
title_full_unstemmed Multifocal imaging for precise, label-free tracking of fast biological processes in 3D
title_sort multifocal imaging for precise, label-free tracking of fast biological processes in 3d
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/6077319b1f614bdbad31c204d6adbb5d
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