Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine

A clorprenaline (CLP) monoclonal antibody, 4G1, raised against the CLP-hapten was successfully prepared for the development of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic assays for the detection of CLP in pig urine samples. The half maximal inhibitory...

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Autores principales:	Wei Jiang, Lu Zeng, Liqiang Liu, Shanshan Song, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	clorprenaline antibody pig urine immunochromatographic strip Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/607b9a049401496bb02ca1489481fe96
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spelling	oai:doaj.org-article:607b9a049401496bb02ca1489481fe962021-11-26T11:19:46ZDevelopment of an immunochromatographic assay for rapid detection of clorprenaline in pig urine0954-01051465-344310.1080/09540105.2017.1411469https://doaj.org/article/607b9a049401496bb02ca1489481fe962018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1411469https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443A clorprenaline (CLP) monoclonal antibody, 4G1, raised against the CLP-hapten was successfully prepared for the development of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic assays for the detection of CLP in pig urine samples. The half maximal inhibitory concentration of the antibody was 0.435 ng/mL, and the limit of detection was 0.104 ng/mL. The linear range was 0.104–1.818 ng/mL, and cross-reactivity with the CLP analogue was <5%. In the spiked sample and recovery test, the recovery ranged from 81.8% to 100.5%, indicating that the ic-ELISA was suitable for CLP analysis in pig urine. The critical value of the immunochromatographic strip in pig urine was 10 ng/mL and could be used for semi-quantitative analysis of CLP. Thus, this immunochromatographic strip was suitable for quickly and sensitively detecting CLP residues in pig urine samples.Wei JiangLu ZengLiqiang LiuShanshan SongHua KuangTaylor & Francis Grouparticleclorprenalineantibodypig urineimmunochromatographic stripAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 536-547 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	clorprenaline antibody pig urine immunochromatographic strip Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	clorprenaline antibody pig urine immunochromatographic strip Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Wei Jiang Lu Zeng Liqiang Liu Shanshan Song Hua Kuang Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine
description	A clorprenaline (CLP) monoclonal antibody, 4G1, raised against the CLP-hapten was successfully prepared for the development of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic assays for the detection of CLP in pig urine samples. The half maximal inhibitory concentration of the antibody was 0.435 ng/mL, and the limit of detection was 0.104 ng/mL. The linear range was 0.104–1.818 ng/mL, and cross-reactivity with the CLP analogue was <5%. In the spiked sample and recovery test, the recovery ranged from 81.8% to 100.5%, indicating that the ic-ELISA was suitable for CLP analysis in pig urine. The critical value of the immunochromatographic strip in pig urine was 10 ng/mL and could be used for semi-quantitative analysis of CLP. Thus, this immunochromatographic strip was suitable for quickly and sensitively detecting CLP residues in pig urine samples.
format	article
author	Wei Jiang Lu Zeng Liqiang Liu Shanshan Song Hua Kuang
author_facet	Wei Jiang Lu Zeng Liqiang Liu Shanshan Song Hua Kuang
author_sort	Wei Jiang
title	Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine
title_short	Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine
title_full	Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine
title_fullStr	Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine
title_full_unstemmed	Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine
title_sort	development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/607b9a049401496bb02ca1489481fe96
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Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine

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