Influence of the temperature and the genotype of the HSP90AA1 gene over sperm chromatin stability in Manchega Rams.

The present study addresses the effect of heat stress on males' reproduction ability. For that, we have evaluated the sperm DNA fragmentation (DFI) by SCSA of ejaculates incubated at 37 °C during 0, 24 and 48 hours after its collection, as a way to mimic the temperature circumstances to which s...

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Autores principales:	Manuel Ramón, Judit Salces-Ortiz, Carmen González, M Dolores Pérez-Guzmán, J Julián Garde, Olga García-Álvarez, Alejandro Maroto-Morales, Jorge H Calvo, M Magdalena Serrano
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/608424be8ea7491f87d0642a67cdbb90
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spelling	oai:doaj.org-article:608424be8ea7491f87d0642a67cdbb902021-11-18T08:36:51ZInfluence of the temperature and the genotype of the HSP90AA1 gene over sperm chromatin stability in Manchega Rams.1932-620310.1371/journal.pone.0086107https://doaj.org/article/608424be8ea7491f87d0642a67cdbb902014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24465903/?tool=EBIhttps://doaj.org/toc/1932-6203The present study addresses the effect of heat stress on males' reproduction ability. For that, we have evaluated the sperm DNA fragmentation (DFI) by SCSA of ejaculates incubated at 37 °C during 0, 24 and 48 hours after its collection, as a way to mimic the temperature circumstances to which spermatozoa will be subject to in the ewe uterus. The effects of temperature and temperature-humidity index (THI) from day 60 prior collection to the date of semen collection on DFI were examined. To better understand the causes determining the sensitivity of spermatozoa to heat, this study was conducted in 60 males with alternative genotypes for the SNP G/C-660 of the HSP90AA1 promoter, which encode for the Hsp90α protein. The Hsp90α protein predominates in the brain and testis, and its role in spermatogenesis has been described in several species. Ridge regression analyses showed that days 29 to 35 and 7 to 14 before sperm collection (bsc) were the most critical regarding the effect of heat stress over DFI values. Mixed model analyses revealed that DFI increases over a threshold of 30 °C for maximum temperature and 22 for THI at days 29 to 35 and 7 to 14 bsc only in animals carrying the GG-660 genotype. The period 29-35 bsc coincide with the meiosis I process for which the effect of the Hsp90α has been described in mice. The period 7-14 bsc may correspond with later stages of the meiosis II and early stages of epididymal maturation in which the replacement of histones by protamines occurs. Because of GG-660 genotype has been associated to lower levels of HSP90AA1 expression, suboptimal amounts of HSP90AA1 mRNA in GG-660 animals under heat stress conditions make spermatozoa DNA more susceptible to be fragmented. Thus, selecting against the GG-660 genotype could decrease the DNA fragmentation and spermatozoa thermal susceptibility in the heat season, and its putative subsequent fertility gains.Manuel RamónJudit Salces-OrtizCarmen GonzálezM Dolores Pérez-GuzmánJ Julián GardeOlga García-ÁlvarezAlejandro Maroto-MoralesJorge H CalvoM Magdalena SerranoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86107 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manuel Ramón Judit Salces-Ortiz Carmen González M Dolores Pérez-Guzmán J Julián Garde Olga García-Álvarez Alejandro Maroto-Morales Jorge H Calvo M Magdalena Serrano Influence of the temperature and the genotype of the HSP90AA1 gene over sperm chromatin stability in Manchega Rams.
description	The present study addresses the effect of heat stress on males' reproduction ability. For that, we have evaluated the sperm DNA fragmentation (DFI) by SCSA of ejaculates incubated at 37 °C during 0, 24 and 48 hours after its collection, as a way to mimic the temperature circumstances to which spermatozoa will be subject to in the ewe uterus. The effects of temperature and temperature-humidity index (THI) from day 60 prior collection to the date of semen collection on DFI were examined. To better understand the causes determining the sensitivity of spermatozoa to heat, this study was conducted in 60 males with alternative genotypes for the SNP G/C-660 of the HSP90AA1 promoter, which encode for the Hsp90α protein. The Hsp90α protein predominates in the brain and testis, and its role in spermatogenesis has been described in several species. Ridge regression analyses showed that days 29 to 35 and 7 to 14 before sperm collection (bsc) were the most critical regarding the effect of heat stress over DFI values. Mixed model analyses revealed that DFI increases over a threshold of 30 °C for maximum temperature and 22 for THI at days 29 to 35 and 7 to 14 bsc only in animals carrying the GG-660 genotype. The period 29-35 bsc coincide with the meiosis I process for which the effect of the Hsp90α has been described in mice. The period 7-14 bsc may correspond with later stages of the meiosis II and early stages of epididymal maturation in which the replacement of histones by protamines occurs. Because of GG-660 genotype has been associated to lower levels of HSP90AA1 expression, suboptimal amounts of HSP90AA1 mRNA in GG-660 animals under heat stress conditions make spermatozoa DNA more susceptible to be fragmented. Thus, selecting against the GG-660 genotype could decrease the DNA fragmentation and spermatozoa thermal susceptibility in the heat season, and its putative subsequent fertility gains.
format	article
author	Manuel Ramón Judit Salces-Ortiz Carmen González M Dolores Pérez-Guzmán J Julián Garde Olga García-Álvarez Alejandro Maroto-Morales Jorge H Calvo M Magdalena Serrano
author_facet	Manuel Ramón Judit Salces-Ortiz Carmen González M Dolores Pérez-Guzmán J Julián Garde Olga García-Álvarez Alejandro Maroto-Morales Jorge H Calvo M Magdalena Serrano
author_sort	Manuel Ramón
title	Influence of the temperature and the genotype of the HSP90AA1 gene over sperm chromatin stability in Manchega Rams.
title_short	Influence of the temperature and the genotype of the HSP90AA1 gene over sperm chromatin stability in Manchega Rams.
title_full	Influence of the temperature and the genotype of the HSP90AA1 gene over sperm chromatin stability in Manchega Rams.
title_fullStr	Influence of the temperature and the genotype of the HSP90AA1 gene over sperm chromatin stability in Manchega Rams.
title_full_unstemmed	Influence of the temperature and the genotype of the HSP90AA1 gene over sperm chromatin stability in Manchega Rams.
title_sort	influence of the temperature and the genotype of the hsp90aa1 gene over sperm chromatin stability in manchega rams.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/608424be8ea7491f87d0642a67cdbb90
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Influence of the temperature and the genotype of the HSP90AA1 gene over sperm chromatin stability in Manchega Rams.

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