Non-thermal plasma-treated solution demonstrates antitumor activity against pancreatic cancer cells in vitro and in vivo

Abstract Pancreatic cancer is associated with a high mortality rate. In advanced stage, patients often experience peritoneal carcinomatosis. Using a syngeneic murine pancreatic cancer cell tumor model, the effect of non-thermal plasma (NTP) on peritoneal metastatic lesions was studied. NTP generates...

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Autores principales:	Kim Rouven Liedtke, Sander Bekeschus, André Kaeding, Christine Hackbarth, Jens-Peter Kuehn, Claus-Dieter Heidecke, Wolfram von Bernstorff, Thomas von Woedtke, Lars Ivo Partecke
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6086b0408efe41279332f0c83aabe9fd
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spelling	oai:doaj.org-article:6086b0408efe41279332f0c83aabe9fd2021-12-02T11:53:05ZNon-thermal plasma-treated solution demonstrates antitumor activity against pancreatic cancer cells in vitro and in vivo10.1038/s41598-017-08560-32045-2322https://doaj.org/article/6086b0408efe41279332f0c83aabe9fd2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08560-3https://doaj.org/toc/2045-2322Abstract Pancreatic cancer is associated with a high mortality rate. In advanced stage, patients often experience peritoneal carcinomatosis. Using a syngeneic murine pancreatic cancer cell tumor model, the effect of non-thermal plasma (NTP) on peritoneal metastatic lesions was studied. NTP generates reactive species of several kinds which have been proven to be of relevance in cancer. In vitro, exposure to both plasma and plasma-treated solution significantly decreased cell viability and proliferation of 6606PDA cancer cells, whereas mouse fibroblasts were less affected. Repeated intraperitoneal treatment of NTP-conditioned medium decreased tumor growth in vivo as determined by magnetic resonance imaging, leading to reduced tumor mass and improved median survival (61 vs 52 days; p < 0.024). Tumor nodes treated by NTP-conditioned medium demonstrated large areas of apoptosis with strongly inhibited cell proliferation. Contemporaneously, no systemic effects were found. Apoptosis was neither present in the liver nor in the gut. Also, the concentration of different cytokines in splenocytes or blood plasma as well as the distribution of various hematological parameters remained unchanged following treatment with NTP-conditioned medium. These results suggest an anticancer role of NTP-treated solutions with little to no systemic side effects being present, making NTP-treated solutions a potential complementary therapeutic option for advanced tumors.Kim Rouven LiedtkeSander BekeschusAndré KaedingChristine HackbarthJens-Peter KuehnClaus-Dieter HeideckeWolfram von BernstorffThomas von WoedtkeLars Ivo ParteckeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kim Rouven Liedtke Sander Bekeschus André Kaeding Christine Hackbarth Jens-Peter Kuehn Claus-Dieter Heidecke Wolfram von Bernstorff Thomas von Woedtke Lars Ivo Partecke Non-thermal plasma-treated solution demonstrates antitumor activity against pancreatic cancer cells in vitro and in vivo
description	Abstract Pancreatic cancer is associated with a high mortality rate. In advanced stage, patients often experience peritoneal carcinomatosis. Using a syngeneic murine pancreatic cancer cell tumor model, the effect of non-thermal plasma (NTP) on peritoneal metastatic lesions was studied. NTP generates reactive species of several kinds which have been proven to be of relevance in cancer. In vitro, exposure to both plasma and plasma-treated solution significantly decreased cell viability and proliferation of 6606PDA cancer cells, whereas mouse fibroblasts were less affected. Repeated intraperitoneal treatment of NTP-conditioned medium decreased tumor growth in vivo as determined by magnetic resonance imaging, leading to reduced tumor mass and improved median survival (61 vs 52 days; p < 0.024). Tumor nodes treated by NTP-conditioned medium demonstrated large areas of apoptosis with strongly inhibited cell proliferation. Contemporaneously, no systemic effects were found. Apoptosis was neither present in the liver nor in the gut. Also, the concentration of different cytokines in splenocytes or blood plasma as well as the distribution of various hematological parameters remained unchanged following treatment with NTP-conditioned medium. These results suggest an anticancer role of NTP-treated solutions with little to no systemic side effects being present, making NTP-treated solutions a potential complementary therapeutic option for advanced tumors.
format	article
author	Kim Rouven Liedtke Sander Bekeschus André Kaeding Christine Hackbarth Jens-Peter Kuehn Claus-Dieter Heidecke Wolfram von Bernstorff Thomas von Woedtke Lars Ivo Partecke
author_facet	Kim Rouven Liedtke Sander Bekeschus André Kaeding Christine Hackbarth Jens-Peter Kuehn Claus-Dieter Heidecke Wolfram von Bernstorff Thomas von Woedtke Lars Ivo Partecke
author_sort	Kim Rouven Liedtke
title	Non-thermal plasma-treated solution demonstrates antitumor activity against pancreatic cancer cells in vitro and in vivo
title_short	Non-thermal plasma-treated solution demonstrates antitumor activity against pancreatic cancer cells in vitro and in vivo
title_full	Non-thermal plasma-treated solution demonstrates antitumor activity against pancreatic cancer cells in vitro and in vivo
title_fullStr	Non-thermal plasma-treated solution demonstrates antitumor activity against pancreatic cancer cells in vitro and in vivo
title_full_unstemmed	Non-thermal plasma-treated solution demonstrates antitumor activity against pancreatic cancer cells in vitro and in vivo
title_sort	non-thermal plasma-treated solution demonstrates antitumor activity against pancreatic cancer cells in vitro and in vivo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6086b0408efe41279332f0c83aabe9fd
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Non-thermal plasma-treated solution demonstrates antitumor activity against pancreatic cancer cells in vitro and in vivo

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