Evaluation of Identification and Susceptibility for Candida Spp. Isolated Directly from Positive Blood Culture Bottles

Determination of the susceptibility profile of isolates of Candida from blood culture bottles is extremely important for correctly guiding patient pharmacotherapy. The aim of this study was to compare the results of analysis of Candida isolated directly from blood culture bottles by the VITEK MS MAL...

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Autores principales:	Ândrea Celestino de Souza, Luciano Z. Goldani, Eliane Würdig Roesch, Larissa Lutz, Patricia Orlandi Barth, Paulo André de Souza Sampaio, Valério Rodrigues Aquino, Dariane Castro Pereira
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/60897ba8fe4a47a2bab95cfbca9734f0
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spelling	oai:doaj.org-article:60897ba8fe4a47a2bab95cfbca9734f02021-11-29T00:55:43ZEvaluation of Identification and Susceptibility for Candida Spp. Isolated Directly from Positive Blood Culture Bottles1687-919810.1155/2021/9364231https://doaj.org/article/60897ba8fe4a47a2bab95cfbca9734f02021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/9364231https://doaj.org/toc/1687-9198Determination of the susceptibility profile of isolates of Candida from blood culture bottles is extremely important for correctly guiding patient pharmacotherapy. The aim of this study was to compare the results of analysis of Candida isolated directly from blood culture bottles by the VITEK MS MALDI-TOF identification system and the fluconazole disk diffusion assay with those of standard identification methods. Testing directly from the bottle allowed results 24 to 48 hours quicker than the standard method. There was a categorical agreement of 51.64% (47 of 91 samples) between the results of analysis directly from the bottle and analysis by the standard method. Regarding species identification, there was 96.15% agreement for Candida parapsilosis (25 of 26 samples). Categorical agreement between the rapid and standard disk diffusion methods was 95%, and the agreement between the rapid disk diffusion method and the broth microdilution method was 97%. Only minor errors in the rapid method were observed: 3 (5%) in the standard disk diffusion method and 2 (3%) in the broth microdilution method. Our study concluded that the rapid disk diffusion method for fluconazole is a fast, easy, reproducible, and consistent method. Its timely implementation for testing antifungal agents in the clinical microbiology laboratory can help reduce profile release times, thus helping to determine the most appropriate antifungal treatment.Ândrea Celestino de SouzaLuciano Z. GoldaniEliane Würdig RoeschLarissa LutzPatricia Orlandi BarthPaulo André de Souza SampaioValério Rodrigues AquinoDariane Castro PereiraHindawi LimitedarticleMicrobiologyQR1-502ENInternational Journal of Microbiology, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Ândrea Celestino de Souza Luciano Z. Goldani Eliane Würdig Roesch Larissa Lutz Patricia Orlandi Barth Paulo André de Souza Sampaio Valério Rodrigues Aquino Dariane Castro Pereira Evaluation of Identification and Susceptibility for Candida Spp. Isolated Directly from Positive Blood Culture Bottles
description	Determination of the susceptibility profile of isolates of Candida from blood culture bottles is extremely important for correctly guiding patient pharmacotherapy. The aim of this study was to compare the results of analysis of Candida isolated directly from blood culture bottles by the VITEK MS MALDI-TOF identification system and the fluconazole disk diffusion assay with those of standard identification methods. Testing directly from the bottle allowed results 24 to 48 hours quicker than the standard method. There was a categorical agreement of 51.64% (47 of 91 samples) between the results of analysis directly from the bottle and analysis by the standard method. Regarding species identification, there was 96.15% agreement for Candida parapsilosis (25 of 26 samples). Categorical agreement between the rapid and standard disk diffusion methods was 95%, and the agreement between the rapid disk diffusion method and the broth microdilution method was 97%. Only minor errors in the rapid method were observed: 3 (5%) in the standard disk diffusion method and 2 (3%) in the broth microdilution method. Our study concluded that the rapid disk diffusion method for fluconazole is a fast, easy, reproducible, and consistent method. Its timely implementation for testing antifungal agents in the clinical microbiology laboratory can help reduce profile release times, thus helping to determine the most appropriate antifungal treatment.
format	article
author	Ândrea Celestino de Souza Luciano Z. Goldani Eliane Würdig Roesch Larissa Lutz Patricia Orlandi Barth Paulo André de Souza Sampaio Valério Rodrigues Aquino Dariane Castro Pereira
author_facet	Ândrea Celestino de Souza Luciano Z. Goldani Eliane Würdig Roesch Larissa Lutz Patricia Orlandi Barth Paulo André de Souza Sampaio Valério Rodrigues Aquino Dariane Castro Pereira
author_sort	Ândrea Celestino de Souza
title	Evaluation of Identification and Susceptibility for Candida Spp. Isolated Directly from Positive Blood Culture Bottles
title_short	Evaluation of Identification and Susceptibility for Candida Spp. Isolated Directly from Positive Blood Culture Bottles
title_full	Evaluation of Identification and Susceptibility for Candida Spp. Isolated Directly from Positive Blood Culture Bottles
title_fullStr	Evaluation of Identification and Susceptibility for Candida Spp. Isolated Directly from Positive Blood Culture Bottles
title_full_unstemmed	Evaluation of Identification and Susceptibility for Candida Spp. Isolated Directly from Positive Blood Culture Bottles
title_sort	evaluation of identification and susceptibility for candida spp. isolated directly from positive blood culture bottles
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/60897ba8fe4a47a2bab95cfbca9734f0
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Evaluation of Identification and Susceptibility for Candida Spp. Isolated Directly from Positive Blood Culture Bottles

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