The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development

The anaphase promoting complex/cyclosome (APC/C), a member of the E3 ubiquitin ligase family, plays an important role in recognizing the substrates to be ubiquitylated. Progression of anaphase, and therefore, of the cell cycle, is coordinated through cyclin degradation cycles dependent on proteolysi...

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Autores principales:	Rodrigo Porto Schwedersky, Marina de Lyra Soriano Saleme, Ingrid Andrade Rocha, Patricia da Fonseca Montessoro, Adriana Silva Hemerly, Nubia Barbosa Eloy, Paulo Cavalcanti Gomes Ferreira
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	plant development cell cycle anaphase promoting complex (APC) organ size meristem cells Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/608cf35d98684091bc4b964d288bc943
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spelling	oai:doaj.org-article:608cf35d98684091bc4b964d288bc9432021-11-30T13:51:16ZThe Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development1664-462X10.3389/fpls.2021.563760https://doaj.org/article/608cf35d98684091bc4b964d288bc9432021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.563760/fullhttps://doaj.org/toc/1664-462XThe anaphase promoting complex/cyclosome (APC/C), a member of the E3 ubiquitin ligase family, plays an important role in recognizing the substrates to be ubiquitylated. Progression of anaphase, and therefore, of the cell cycle, is coordinated through cyclin degradation cycles dependent on proteolysis triggered by APC/C. The APC/C activity depends on the formation of a pocket comprising the catalytic subunits, APC2, APC11, and APC10. Among these, the role of APC11 outside the cell division cycle is poorly understood. Therefore, the goal of this work was to analyze the function of APC11 during plant development by characterizing apc11 knock-down mutant lines. Accordingly, we observed decreased apc11 expression in the mutant lines, followed by a reduction in meristem root size based on the cortical cell length, and an overall size diminishment throughout the development. Additionally, crosses of apc11-1 and amiR-apc11 with plants carrying a WUSCHEL-RELATED HOMEOBOX5 (WOX5) fluorescent marker showed a weakening of the green fluorescent protein-positive cells in the Quiescent Center. Moreover, plants with apc11-1 show a decreased leaf area, together with a decrease in the cell area when the shoot development was observed by kinematics analysis. Finally, we observed a decreased APC/C activity in the root and shoot meristems in crosses of pCYCB1;1:D-box-GUS with apc11-1 plants. Our results indicate that APC11 is important in the early stages of development, mediating meristematic architecture through APC/C activity affecting the overall plant growth.Rodrigo Porto SchwederskyMarina de Lyra Soriano SalemeIngrid Andrade RochaPatricia da Fonseca MontessoroAdriana Silva HemerlyNubia Barbosa EloyPaulo Cavalcanti Gomes FerreiraFrontiers Media S.A.articleplant developmentcell cycleanaphase promoting complex (APC)organ sizemeristem cellsPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plant development cell cycle anaphase promoting complex (APC) organ size meristem cells Plant culture SB1-1110
spellingShingle	plant development cell cycle anaphase promoting complex (APC) organ size meristem cells Plant culture SB1-1110 Rodrigo Porto Schwedersky Marina de Lyra Soriano Saleme Ingrid Andrade Rocha Patricia da Fonseca Montessoro Adriana Silva Hemerly Nubia Barbosa Eloy Paulo Cavalcanti Gomes Ferreira The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development
description	The anaphase promoting complex/cyclosome (APC/C), a member of the E3 ubiquitin ligase family, plays an important role in recognizing the substrates to be ubiquitylated. Progression of anaphase, and therefore, of the cell cycle, is coordinated through cyclin degradation cycles dependent on proteolysis triggered by APC/C. The APC/C activity depends on the formation of a pocket comprising the catalytic subunits, APC2, APC11, and APC10. Among these, the role of APC11 outside the cell division cycle is poorly understood. Therefore, the goal of this work was to analyze the function of APC11 during plant development by characterizing apc11 knock-down mutant lines. Accordingly, we observed decreased apc11 expression in the mutant lines, followed by a reduction in meristem root size based on the cortical cell length, and an overall size diminishment throughout the development. Additionally, crosses of apc11-1 and amiR-apc11 with plants carrying a WUSCHEL-RELATED HOMEOBOX5 (WOX5) fluorescent marker showed a weakening of the green fluorescent protein-positive cells in the Quiescent Center. Moreover, plants with apc11-1 show a decreased leaf area, together with a decrease in the cell area when the shoot development was observed by kinematics analysis. Finally, we observed a decreased APC/C activity in the root and shoot meristems in crosses of pCYCB1;1:D-box-GUS with apc11-1 plants. Our results indicate that APC11 is important in the early stages of development, mediating meristematic architecture through APC/C activity affecting the overall plant growth.
format	article
author	Rodrigo Porto Schwedersky Marina de Lyra Soriano Saleme Ingrid Andrade Rocha Patricia da Fonseca Montessoro Adriana Silva Hemerly Nubia Barbosa Eloy Paulo Cavalcanti Gomes Ferreira
author_facet	Rodrigo Porto Schwedersky Marina de Lyra Soriano Saleme Ingrid Andrade Rocha Patricia da Fonseca Montessoro Adriana Silva Hemerly Nubia Barbosa Eloy Paulo Cavalcanti Gomes Ferreira
author_sort	Rodrigo Porto Schwedersky
title	The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development
title_short	The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development
title_full	The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development
title_fullStr	The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development
title_full_unstemmed	The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development
title_sort	anaphase promoting complex/cyclosome subunit 11 and its role in organ size and plant development
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/608cf35d98684091bc4b964d288bc943
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The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development

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