Pig α1-acid glycoprotein: characterization and first description in any species as a negative acute phase protein.

The serum protein α1-acid glycoprotein (AGP), also known as orosomucoid, is generally described as an archetypical positive acute phase protein. Here, porcine AGP was identified, purified and characterized from pooled pig serum. It was found to circulate as a single chain glycoprotein having an appa...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Peter M H Heegaard, Ingrid Miller, Nanna Skall Sorensen, Karen Elisabeth Soerensen, Kerstin Skovgaard
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/60914d688d394c12aead9cef033d3c2c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:60914d688d394c12aead9cef033d3c2c
record_format	dspace
spelling	oai:doaj.org-article:60914d688d394c12aead9cef033d3c2c2021-11-18T07:38:59ZPig α1-acid glycoprotein: characterization and first description in any species as a negative acute phase protein.1932-620310.1371/journal.pone.0068110https://doaj.org/article/60914d688d394c12aead9cef033d3c2c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23844161/?tool=EBIhttps://doaj.org/toc/1932-6203The serum protein α1-acid glycoprotein (AGP), also known as orosomucoid, is generally described as an archetypical positive acute phase protein. Here, porcine AGP was identified, purified and characterized from pooled pig serum. It was found to circulate as a single chain glycoprotein having an apparent molecular weight of 43 kDa by SDS-PAGE under reducing conditions, of which approximately 17 kDa were accounted for by N-bound oligosaccharides. Those data correspond well with the properties of the protein predicted from the single porcine AGP gene (ORM1, Q29014 (UniProt)), containing 5 putative glycosylation sites. A monoclonal antibody (MAb) was produced and shown to quantitatively and specifically react with all microheterogenous forms of pig AGP as analyzed by 2-D electrophoresis. This MAb was used to develop an immunoassay (ELISA) for quantification of AGP in pig serum samples. The adult serum concentrations of pig AGP were in the range of 1-3 mg/ml in a number of conventional pig breeds while it was lower in Göttingen and Ossabaw minipigs (in the 0.3 to 0.6 mg/ml range) and higher in young (2-5 days old) conventional pigs (mean: 6.6 mg/ml). Surprisingly, pig AGP was found to behave as a negative acute phase protein during a range of experimental infections and aseptic inflammation with significant decreases in serum concentration and in hepatic ORM1 expression during the acute phase response. To our knowledge this is the first description in any species of AGP being a negative acute phase protein.Peter M H HeegaardIngrid MillerNanna Skall SorensenKaren Elisabeth SoerensenKerstin SkovgaardPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68110 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peter M H Heegaard Ingrid Miller Nanna Skall Sorensen Karen Elisabeth Soerensen Kerstin Skovgaard Pig α1-acid glycoprotein: characterization and first description in any species as a negative acute phase protein.
description	The serum protein α1-acid glycoprotein (AGP), also known as orosomucoid, is generally described as an archetypical positive acute phase protein. Here, porcine AGP was identified, purified and characterized from pooled pig serum. It was found to circulate as a single chain glycoprotein having an apparent molecular weight of 43 kDa by SDS-PAGE under reducing conditions, of which approximately 17 kDa were accounted for by N-bound oligosaccharides. Those data correspond well with the properties of the protein predicted from the single porcine AGP gene (ORM1, Q29014 (UniProt)), containing 5 putative glycosylation sites. A monoclonal antibody (MAb) was produced and shown to quantitatively and specifically react with all microheterogenous forms of pig AGP as analyzed by 2-D electrophoresis. This MAb was used to develop an immunoassay (ELISA) for quantification of AGP in pig serum samples. The adult serum concentrations of pig AGP were in the range of 1-3 mg/ml in a number of conventional pig breeds while it was lower in Göttingen and Ossabaw minipigs (in the 0.3 to 0.6 mg/ml range) and higher in young (2-5 days old) conventional pigs (mean: 6.6 mg/ml). Surprisingly, pig AGP was found to behave as a negative acute phase protein during a range of experimental infections and aseptic inflammation with significant decreases in serum concentration and in hepatic ORM1 expression during the acute phase response. To our knowledge this is the first description in any species of AGP being a negative acute phase protein.
format	article
author	Peter M H Heegaard Ingrid Miller Nanna Skall Sorensen Karen Elisabeth Soerensen Kerstin Skovgaard
author_facet	Peter M H Heegaard Ingrid Miller Nanna Skall Sorensen Karen Elisabeth Soerensen Kerstin Skovgaard
author_sort	Peter M H Heegaard
title	Pig α1-acid glycoprotein: characterization and first description in any species as a negative acute phase protein.
title_short	Pig α1-acid glycoprotein: characterization and first description in any species as a negative acute phase protein.
title_full	Pig α1-acid glycoprotein: characterization and first description in any species as a negative acute phase protein.
title_fullStr	Pig α1-acid glycoprotein: characterization and first description in any species as a negative acute phase protein.
title_full_unstemmed	Pig α1-acid glycoprotein: characterization and first description in any species as a negative acute phase protein.
title_sort	pig α1-acid glycoprotein: characterization and first description in any species as a negative acute phase protein.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/60914d688d394c12aead9cef033d3c2c
work_keys_str_mv	AT petermhheegaard piga1acidglycoproteincharacterizationandfirstdescriptioninanyspeciesasanegativeacutephaseprotein AT ingridmiller piga1acidglycoproteincharacterizationandfirstdescriptioninanyspeciesasanegativeacutephaseprotein AT nannaskallsorensen piga1acidglycoproteincharacterizationandfirstdescriptioninanyspeciesasanegativeacutephaseprotein AT karenelisabethsoerensen piga1acidglycoproteincharacterizationandfirstdescriptioninanyspeciesasanegativeacutephaseprotein AT kerstinskovgaard piga1acidglycoproteincharacterizationandfirstdescriptioninanyspeciesasanegativeacutephaseprotein
_version_	1718423163375714304

Pig α1-acid glycoprotein: characterization and first description in any species as a negative acute phase protein.

Ejemplares similares