Digital microfluidic processing of mammalian embryos for vitrification.

Cryopreservation is a key technology in biology and clinical practice. This paper presents a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual micro droplets manipulated on the microfluidic device were used as micro-vessels to transport a si...

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Autores principales:	Derek G Pyne, Jun Liu, Mohamed Abdelgawad, Yu Sun
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/60964fe9066040afaa5378544c5e6619
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spelling	oai:doaj.org-article:60964fe9066040afaa5378544c5e66192021-11-25T05:59:36ZDigital microfluidic processing of mammalian embryos for vitrification.1932-620310.1371/journal.pone.0108128https://doaj.org/article/60964fe9066040afaa5378544c5e66192014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108128https://doaj.org/toc/1932-6203Cryopreservation is a key technology in biology and clinical practice. This paper presents a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual micro droplets manipulated on the microfluidic device were used as micro-vessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos.Derek G PyneJun LiuMohamed AbdelgawadYu SunPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e108128 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Derek G Pyne Jun Liu Mohamed Abdelgawad Yu Sun Digital microfluidic processing of mammalian embryos for vitrification.
description	Cryopreservation is a key technology in biology and clinical practice. This paper presents a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual micro droplets manipulated on the microfluidic device were used as micro-vessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos.
format	article
author	Derek G Pyne Jun Liu Mohamed Abdelgawad Yu Sun
author_facet	Derek G Pyne Jun Liu Mohamed Abdelgawad Yu Sun
author_sort	Derek G Pyne
title	Digital microfluidic processing of mammalian embryos for vitrification.
title_short	Digital microfluidic processing of mammalian embryos for vitrification.
title_full	Digital microfluidic processing of mammalian embryos for vitrification.
title_fullStr	Digital microfluidic processing of mammalian embryos for vitrification.
title_full_unstemmed	Digital microfluidic processing of mammalian embryos for vitrification.
title_sort	digital microfluidic processing of mammalian embryos for vitrification.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/60964fe9066040afaa5378544c5e6619
work_keys_str_mv	AT derekgpyne digitalmicrofluidicprocessingofmammalianembryosforvitrification AT junliu digitalmicrofluidicprocessingofmammalianembryosforvitrification AT mohamedabdelgawad digitalmicrofluidicprocessingofmammalianembryosforvitrification AT yusun digitalmicrofluidicprocessingofmammalianembryosforvitrification
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Digital microfluidic processing of mammalian embryos for vitrification.

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