Cross-reactivity of herpesvirus-specific CD8 T cell lines toward allogeneic class I MHC molecules.

Although association between persistent viral infection and allograft rejection is well characterized, few examples of T-cell cross-reactivity between self-MHC/viral and allogeneic HLA molecules have been documented so far. We appraised in this study the alloreactivity of CD8 T cell lines specific f...

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Autores principales:	Alexis Morice, Béatrice Charreau, Bérangère Neveu, Sophie Brouard, Jean-Paul Soulillou, Marc Bonneville, Elisabeth Houssaint, Nicolas Degauque
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6098026515e74fe6bb5a3ccacffdeedb
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spelling	oai:doaj.org-article:6098026515e74fe6bb5a3ccacffdeedb2021-11-18T06:36:07ZCross-reactivity of herpesvirus-specific CD8 T cell lines toward allogeneic class I MHC molecules.1932-620310.1371/journal.pone.0012120https://doaj.org/article/6098026515e74fe6bb5a3ccacffdeedb2010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20711433/?tool=EBIhttps://doaj.org/toc/1932-6203Although association between persistent viral infection and allograft rejection is well characterized, few examples of T-cell cross-reactivity between self-MHC/viral and allogeneic HLA molecules have been documented so far. We appraised in this study the alloreactivity of CD8 T cell lines specific for immunodominant epitopes from human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV). CD8 T cell lines were generated after sorting with immunomagnetic beads coated with either pp65(495-503)/A0201, BMLF1(259-267)/A0201, or BZLF1(54-64)/B3501 multimeric complexes. Alloreactivity of the CD8 T cell lines against allogeneic class I MHC alleles was assessed by screening of (i) TNF-alpha production against COS-7 cells transfected with as many as 39 individual HLA class I-encoding cDNA, and (ii) cytotoxicity activity toward a large panel of HLA-typed EBV-transformed B lymphoblastoid cell lines. We identified several cross-reactive pp65/A0201-specific T cell lines toward allogeneic HLA-A3001, A3101, or A3201. Moreover, we described here cross-recognition of HLA-Cw0602 by BZLF1/B*3501-specific T cells. It is noteworthy that these alloreactive CD8 T cell lines showed efficient recognition of endothelial cells expressing the relevant HLA class I allele, with high level TNF-alpha production and cytotoxicity activity. Taken together, our data support the notion that herpes virus-specific T cells recognizing allo-HLA alleles may promote solid organ rejection.Alexis MoriceBéatrice CharreauBérangère NeveuSophie BrouardJean-Paul SoulillouMarc BonnevilleElisabeth HoussaintNicolas DegauquePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 8, p e12120 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexis Morice Béatrice Charreau Bérangère Neveu Sophie Brouard Jean-Paul Soulillou Marc Bonneville Elisabeth Houssaint Nicolas Degauque Cross-reactivity of herpesvirus-specific CD8 T cell lines toward allogeneic class I MHC molecules.
description	Although association between persistent viral infection and allograft rejection is well characterized, few examples of T-cell cross-reactivity between self-MHC/viral and allogeneic HLA molecules have been documented so far. We appraised in this study the alloreactivity of CD8 T cell lines specific for immunodominant epitopes from human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV). CD8 T cell lines were generated after sorting with immunomagnetic beads coated with either pp65(495-503)/A0201, BMLF1(259-267)/A0201, or BZLF1(54-64)/B3501 multimeric complexes. Alloreactivity of the CD8 T cell lines against allogeneic class I MHC alleles was assessed by screening of (i) TNF-alpha production against COS-7 cells transfected with as many as 39 individual HLA class I-encoding cDNA, and (ii) cytotoxicity activity toward a large panel of HLA-typed EBV-transformed B lymphoblastoid cell lines. We identified several cross-reactive pp65/A0201-specific T cell lines toward allogeneic HLA-A3001, A3101, or A3201. Moreover, we described here cross-recognition of HLA-Cw0602 by BZLF1/B*3501-specific T cells. It is noteworthy that these alloreactive CD8 T cell lines showed efficient recognition of endothelial cells expressing the relevant HLA class I allele, with high level TNF-alpha production and cytotoxicity activity. Taken together, our data support the notion that herpes virus-specific T cells recognizing allo-HLA alleles may promote solid organ rejection.
format	article
author	Alexis Morice Béatrice Charreau Bérangère Neveu Sophie Brouard Jean-Paul Soulillou Marc Bonneville Elisabeth Houssaint Nicolas Degauque
author_facet	Alexis Morice Béatrice Charreau Bérangère Neveu Sophie Brouard Jean-Paul Soulillou Marc Bonneville Elisabeth Houssaint Nicolas Degauque
author_sort	Alexis Morice
title	Cross-reactivity of herpesvirus-specific CD8 T cell lines toward allogeneic class I MHC molecules.
title_short	Cross-reactivity of herpesvirus-specific CD8 T cell lines toward allogeneic class I MHC molecules.
title_full	Cross-reactivity of herpesvirus-specific CD8 T cell lines toward allogeneic class I MHC molecules.
title_fullStr	Cross-reactivity of herpesvirus-specific CD8 T cell lines toward allogeneic class I MHC molecules.
title_full_unstemmed	Cross-reactivity of herpesvirus-specific CD8 T cell lines toward allogeneic class I MHC molecules.
title_sort	cross-reactivity of herpesvirus-specific cd8 t cell lines toward allogeneic class i mhc molecules.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/6098026515e74fe6bb5a3ccacffdeedb
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Cross-reactivity of herpesvirus-specific CD8 T cell lines toward allogeneic class I MHC molecules.

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